Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 31 - 47 of 47

Full-Text Articles in Life Sciences

The Diel Dynamics Of Ciliate Community In A Tide-Pool, Chien-Fu Chao, Bo-Wei Wang, Chao-Hsiung Cheng, Kuo-Ping Chiang Dec 2013

The Diel Dynamics Of Ciliate Community In A Tide-Pool, Chien-Fu Chao, Bo-Wei Wang, Chao-Hsiung Cheng, Kuo-Ping Chiang

Journal of Marine Science and Technology

We investigated the diel cycle mechanism of a tide-pool ciliate community of the northeastern coast of Taiwan. The investigation was conducted on 17th-18th June (typical summer weather), and on 23th -24th July 2013 (10 days after a typhoon). In June, the tide-pool ecosystem was an independent and the excystment of ciliates in the tide-pool caused a dramatic increase in population abundance, as well as composition change. In July, there was no significant difference between the species composition in the tide-pool and that in the surrounding coastal water. However, the abundance of ciliates in the tide-pool was lower due to the …


Alternative Assessment Methods Applied To The Hairtail (Trichiurus Lepturus) Stock In The Aru Sea As An Example, Chao-Hsiung Cheng, Tsuyoshi Kawasaki, Kuo-Ping Chiang, Chuan-Hung Ho Dec 2013

Alternative Assessment Methods Applied To The Hairtail (Trichiurus Lepturus) Stock In The Aru Sea As An Example, Chao-Hsiung Cheng, Tsuyoshi Kawasaki, Kuo-Ping Chiang, Chuan-Hung Ho

Journal of Marine Science and Technology

The Aru Sea off Indonesia is a major fishing ground for Taiwanese distant-water otter trawlers. The hairtail, an economically important demersal fish, is abundant in that area. The assessment methodology in this paper extends previous studies on hairtail fisheries by considering migration within the fishing grounds. This model is based on conventional Leslie-Delury method but makes different assumptions on stock movement and the relationship between stock abundance and catch per unit effort. It was applied to retrospective data from the hairtail fisheries in the Aru Sea. In addition, the depletion method was used to estimate the abundance of hairtail and …


A New Parapercis Species From The Dongsha Island, South China Sea With Comments On A New Record From Taiwan, I-Shiung Chen, Tsung-Hsien Tsai, Shao-Liang Hsu Dec 2013

A New Parapercis Species From The Dongsha Island, South China Sea With Comments On A New Record From Taiwan, I-Shiung Chen, Tsung-Hsien Tsai, Shao-Liang Hsu

Journal of Marine Science and Technology

The sandperch genus, Parapercis Bleeker, 1863 is the most diverse group of Family Pinguipedidae in the perciforms. One unusual specimen of sandperches recently collected from the Dongsha Island (Pratas Island), South China Sea from 2011 expedition belonging to Taiwan, the Republic of China is turned to a new species as Parapercis dongshaensis n. sp. closely related to the newly recorded species, Parapercis okamurai Kamohara, 1960 which also firstly collected from Taiwanese waters would been also documented herein. The new species, Parapercis dongshaensis n. sp., can be well distinguished from other congeners by the following unique combination of features: (1) fins: …


Identification And Analysis Of White Spot Syndrome Virus (Wssv) Envelope Protein Vp41a, Po-Yu Huang, Pei-Jung Chen, Huai-Ting Huang, Li-Li Chen Dec 2013

Identification And Analysis Of White Spot Syndrome Virus (Wssv) Envelope Protein Vp41a, Po-Yu Huang, Pei-Jung Chen, Huai-Ting Huang, Li-Li Chen

Journal of Marine Science and Technology

White spot syndrome virus (WSSV) is the causative agent of a disease that has led to severe mortalities of cultured shrimp in Taiwan and many other countries. WSSV, a kind of large enveloped DNA virus, has a wide host range among crustaceans. During the first stage to infect a target cell, the enveloped virus must combine itself with the receptor on the host cell membrane via an envelope protein. In WSSV, binding of envelope protein with the receptor of the host cell membrane was revealed in a number of previous studies. WSSV envelope proteins were also considered to form a …


How Important Is Viral Effect On The Measure Bacterial Growth In Western Subtropical Pacific Coastal Water?, An-Yi Tsai, Gwo-Ching Gong, Kuo-Ping Chiang, Jie Hung Dec 2013

How Important Is Viral Effect On The Measure Bacterial Growth In Western Subtropical Pacific Coastal Water?, An-Yi Tsai, Gwo-Ching Gong, Kuo-Ping Chiang, Jie Hung

Journal of Marine Science and Technology

Bacterial growth rates were determined in natural seawater samples using two different methodological approaches: incubation in dialysis bags, and a modification of the dilution approach. Bacterial growth rates estimated for the dialysis bag varied from 0.026 h-1 (April) to 0.208 h-1 (August). Bacterial growth rates in 0.2 µm and 30 kDa size-fractionated series ranged from 0.038 to 0.094 h-1 and 0.078 to 0.42 h-1, respectively. Furthermore, in all of our samples, growth rates in the 30 kDa fractionated series (grazer and virus-free diluent) were higher than in the 0.2 µm series (grazer-free diluent) or in the dialysis bag incubations.


Predation By Apocyclops Royi (Cyclopoid: Copepod) On Ciliates And Rotifers, Raunak Dhanker, Jiang-Shiou Hwang Dec 2013

Predation By Apocyclops Royi (Cyclopoid: Copepod) On Ciliates And Rotifers, Raunak Dhanker, Jiang-Shiou Hwang

Journal of Marine Science and Technology

We studied the prey consumption rates in Apocyclops royi on neonate and adult rotifers, Brachionus rotundiformis and ciliate, Euplotes sp. in the presence and absence of algae, the smaller Isochrysis galbana and the larger Tetraselmis chui. Both males and females of A. royi were able to successfully ingest neonate and adult rotifers and ciliate. The prey consumption rates were significantly lower in males than in females of A. royi. Regardless of sex and reproductive state of the copepod, prey consumption rates were higher on neonates rotifer than on adults. However, the prey consumption rates were significantly lower on ciliate than …


Dna Barcoding Of Coastal Larval Fish Communities Of Dongshar Island, South China Sea Revealed By Mitochondrial Co I Sequences, I-Shiung Chen, Kwang-Tsao Shao, Shao-Liang Hsu, Gwo-Ching Gong, Yu-Chih Chen, Ta-Kang Liu Dec 2013

Dna Barcoding Of Coastal Larval Fish Communities Of Dongshar Island, South China Sea Revealed By Mitochondrial Co I Sequences, I-Shiung Chen, Kwang-Tsao Shao, Shao-Liang Hsu, Gwo-Ching Gong, Yu-Chih Chen, Ta-Kang Liu

Journal of Marine Science and Technology

The 70 different samples of larval fish morphotypes have been successfully amplified and sequenced in detail via PCR amplification for the partial sequences of mtDNA CO I gene. The overall genetic similarities ranged from 83% to 100%. Totally 26 different fish species can be detected and analyzed. There are 16 fish species can be identified into specific level. However, there are remaining 12 species can be expected just into suggested fish generic level. Apparently, two major groups of taxa would be the members of Gobiidae and Apogonidae which comprised 50% of total species diversity, and also comprised 67% of total …


Mosquitocidal Activity Of Hedychium Coronarium Rhizome Extract And Copepod Megacyclops Formosanus For The Control Of Dengue Vector, Kandasamy Kalimuthu, Kadarkarai Murugan, Li-Chun Tseng, Jiang-Shiou Hwang Dec 2013

Mosquitocidal Activity Of Hedychium Coronarium Rhizome Extract And Copepod Megacyclops Formosanus For The Control Of Dengue Vector, Kandasamy Kalimuthu, Kadarkarai Murugan, Li-Chun Tseng, Jiang-Shiou Hwang

Journal of Marine Science and Technology

The bio-efficacy of Hedychium coronarium Rhizome extract and copepods Megacyclops formosanus, were tested against first to fourth-instar larvae and pupae of dengue Vector Aedes aegypti under laboratory conditions. Different solvent extracts of H. coronarium, combined with copepod M. formosanus showed considerable mortality against A. aegypti. The median lethal concentration value (LC50) observed for the larvicidal and pupicidal activities against mosquito vector species A. aegypti value were 38.59, ppm; no mortality was observed in the control group. This study was also initiated to test the predatory efficiency of copepod against different larval instars and predatory efficiency was noticed at the laboratory …


Does High Organic Matter Content Affect Polychaete Assemblages In A Shenzhen Bay Mudflat, China?, Li-Zhe Cai, Jiang-Shiou Hwang, Hans-Uwe Dahms, Su-Jing Fu, Xin-Wei Chen, Chen Wu Dec 2013

Does High Organic Matter Content Affect Polychaete Assemblages In A Shenzhen Bay Mudflat, China?, Li-Zhe Cai, Jiang-Shiou Hwang, Hans-Uwe Dahms, Su-Jing Fu, Xin-Wei Chen, Chen Wu

Journal of Marine Science and Technology

Coastal areas are the first marine systems being impacted by anthropogenic pollution and eutrophication. Organic matter poses a particularly negative impact on the environment due to its oxygen depleting and eutrophying effects. Shenzhen Bay mudflat contains rich organic matter. Here, we compare changes of polychaete assemblage parameters on seasonal data obtained from a Shenzhen Bay mudflat in a three-year study. The results based on the seasonal and spatial variations of polychaete species number, density and biomass, ShannonWiener diversity index, evenness index and richness index confirmed that high organic matter content impacted their distribution. A significant negative correlation between the density …


Diel Oscillations In Sailfish Vertical Movement Behavior In The East China Sea, Wei-Chuan Chiang, Ryo Kawabe, Michael K. Musyl, Chi-Lu Sun, Hsiao-Min Hung, Hsien-Chung Lin, Shun Watanabe, Seishiro Furukawa, Wen-Yie Chen, Yu-Kai Chen, Don-Chung Liu Dec 2013

Diel Oscillations In Sailfish Vertical Movement Behavior In The East China Sea, Wei-Chuan Chiang, Ryo Kawabe, Michael K. Musyl, Chi-Lu Sun, Hsiao-Min Hung, Hsien-Chung Lin, Shun Watanabe, Seishiro Furukawa, Wen-Yie Chen, Yu-Kai Chen, Don-Chung Liu

Journal of Marine Science and Technology

A sailfish (Istiophorus platypterus) was tagged with a pop-up satellite archival tag off the eastern coast of Taiwan and moved in a northerly direction to the East China Sea, where the tag popped-up after 160 days. The total linear displacement was 550 km from deployment to pop-up location and all movements were confined to the East China Sea. After the primarily southward movement during first two months at-liberty, the sailfish changed course after September and began to swim in a northerly direction paralleling the Kuroshio Current. During these horizontal movements, the tagged animal exhibited diel oscillations in its vertical diving …


Genetic Diversity Of Pyramimonas From Ryukyu Archipelago, Japan (Chlorophyceae, Pyramimonadales), Shoichiro Suda, Mohammad Azmal Hossain Bhuiyan, Daphne Georgina Faria Dec 2013

Genetic Diversity Of Pyramimonas From Ryukyu Archipelago, Japan (Chlorophyceae, Pyramimonadales), Shoichiro Suda, Mohammad Azmal Hossain Bhuiyan, Daphne Georgina Faria

Journal of Marine Science and Technology

The genus Pyramimonas Schmarda was traditionally described on observations of its periplast and internal structure of different flagellar apparatus or eyespot orientations and currently comprises of ca. 60 species that are divided into six subgenera: Pyramimonas, Vestigifera, Trichocystis, Punctatae, Hexactis, and Macrura. In order to understand the genetic diversity and phylogenetic relationships of genus Pyramimonas members, we analyzed nuclear SSU rDNA molecular data from 41 strains isolated from different locations of the Ryukyu Archipelago. Phylogenetic analyses revealed that strains could be segregated into six clades, four of which represented existing subgenera: Pyramimonas, Vestigifera McFadden, Trichocystis McFadden, and Punctatae McFadden, and …


Life History Of The Copepod Paramphiascella Sp. Affected By Hydrothermal Vent Effluents, Hans-Uwe Dahms, Li-Chun Tseng, Jiang-Shiou Hwang Dec 2013

Life History Of The Copepod Paramphiascella Sp. Affected By Hydrothermal Vent Effluents, Hans-Uwe Dahms, Li-Chun Tseng, Jiang-Shiou Hwang

Journal of Marine Science and Technology

Toxicity of Hydrothermal vent (HV) effluents was tested by investigating the effect on growth and reproduction of the copepod Paramphiascella sp. which was collected and subsequently cultured from localities nearby a shallow marine HV at Kueishan Tao Island, Taiwan. Ontogenetic stages (nauplii, copepodids, and adults) were exposed to a range of concentrations of HV effluents in a static renewal culture system. In a first of two experiments we tested the survivorship of these in HV effluent dilutions from 1 to 50%. HV effluents significantly reduced the survivorship of the naupliar stages at concentrations >5% for Paramphiascella sp. (p < 0.01) and all nauplii died at concentrations of 25% and 50%. Copepodids were significantly letally affected at concentrations >5% (p < 0.01) and all died at 50% (p < 0.01). Developmental duration in Paramphiascella was showing a trend of developmental delay in both phases, in the naupliar and in the copepodid phase. Mortality showed a greater sensitivity to chemical exposure than development time. Among both traits were early developmental stages of Paramphiascella sp. more sensitive to HV effluents than advanced stages. We showed that Paramphiascella sp. was a useful test organism in the monitoring of life cycle as well as acute effects of HV effluents being present for millions of years and providing unique ecosystems. Mortality was a useful toxicological endpoint whereas developmental duration was not.


Gall Polymorphism Of Coral-Inhabiting Crabs (Decapoda, Cryptochiridae): A New Perspective, Tsui-Ping Wei, Hon-Cheng Chen, Ying-Chou Lee, Min-Li Tsai, Jiang-Shiou Hwang, Shao-Hung Peng, Yuh-Wen Chiu Dec 2013

Gall Polymorphism Of Coral-Inhabiting Crabs (Decapoda, Cryptochiridae): A New Perspective, Tsui-Ping Wei, Hon-Cheng Chen, Ying-Chou Lee, Min-Li Tsai, Jiang-Shiou Hwang, Shao-Hung Peng, Yuh-Wen Chiu

Journal of Marine Science and Technology

Coral-inhabiting gall crabs are either obligate symbionts or parasitic associates with their host corals. They form a variety of galls/pits inside the skeleton of living corals. Nine genera of gall crabs on several scleractinian corals were used to test the hypothesis that galls vary among cryptochirid genera. Phylogenetic and morphometric observations were combined to analyze the possible evolutionary significance of gall construction. A high degree of conservation of gall shapes was observed in relation to the gall crabs’ phylogeny. Gall/pit morphology and fidelity was studied in each of the different species of gall crabs. In addition, the correlation analysis results …


Mosquito Larvicidal Activity Of Broussonetia Papyrifera Compound Marmesin By Blocking Protein Aescp-2, Docking Strategies, And Combined Effect Of Copepod Megacyclops Formosanus Against Dengue Vector Aedes Aegypti (Diptera: Culicidae), Kandasamy Kalimuthu, Chia-Hsiang Wang, Shiu-Mei Liu, Li-Chun Tseng, Kadarkarai Murugan, Jiang-Shiou Hwang Dec 2013

Mosquito Larvicidal Activity Of Broussonetia Papyrifera Compound Marmesin By Blocking Protein Aescp-2, Docking Strategies, And Combined Effect Of Copepod Megacyclops Formosanus Against Dengue Vector Aedes Aegypti (Diptera: Culicidae), Kandasamy Kalimuthu, Chia-Hsiang Wang, Shiu-Mei Liu, Li-Chun Tseng, Kadarkarai Murugan, Jiang-Shiou Hwang

Journal of Marine Science and Technology

Mosquito-borne diseases that have an economic impact create losses in commercial and labor outputs, particularly in countries with tropical and subtropical climates. The emergence of resistance to synthetic insecticides is a challenge to mosquito control. Cyclopoid copepods are important predators in many aquatic ecosystems and have been successfully used as biological agents to control mosquito larvae. For this study, we examined the larvicidal activity of the copepod Megacyclops formosanus in combination with the compound marmesin (which was purified from the methanol crude extract of the plant stem bark of Broussonetia papyrifera) against Aedes aegypti larvae. Their larvicidal activity and in …


The Checklist Of Inland-Water And Mangrove Fish Fauna Of Kinmen Island, Fujian Province, Taiwan With Comments On Ecological Conservation Of Native Fishes, I-Shiung Chen, Chia-Jun Weng, Yi-Ru Chen, Shih-Pin Huang, Zong-Han Wen, Nian-Hong Jang-Liaw, Tsung-Hsien Tsai Dec 2013

The Checklist Of Inland-Water And Mangrove Fish Fauna Of Kinmen Island, Fujian Province, Taiwan With Comments On Ecological Conservation Of Native Fishes, I-Shiung Chen, Chia-Jun Weng, Yi-Ru Chen, Shih-Pin Huang, Zong-Han Wen, Nian-Hong Jang-Liaw, Tsung-Hsien Tsai

Journal of Marine Science and Technology

The ecological status of the lowland freshwater and brackish fish fauna traditionally got better protected since long time for military protect region without great human and agriculture impact in last few decades. However, many human reconstruction of river habitats, pollution and overuse of irrigation from river water have become great impact for fish survival in Kinmen. So far, there are totally 27 families, 57 genera and 68 species have been collected and recorded by our intensive survey for those freshwater habitats in inlandwater of Kinmen island, Fujian Province, Taiwan in our recent field survey during 2011-2012. Totally 17 newly recorded …


Decadal Variation In Egg Abundance Of A Mesopelagic Fish, Maurolicus Japonicus, In The Japan Sea During 1981-2005, Tadanori Fujino, Tsuneo Goto, Tsuyoshi Shimura, Hiroki Yasuma, Yongjun Tian, Hideaki Kidokoro, Shinya Masuda, Kazushi Miyashita Feb 2013

Decadal Variation In Egg Abundance Of A Mesopelagic Fish, Maurolicus Japonicus, In The Japan Sea During 1981-2005, Tadanori Fujino, Tsuneo Goto, Tsuyoshi Shimura, Hiroki Yasuma, Yongjun Tian, Hideaki Kidokoro, Shinya Masuda, Kazushi Miyashita

Journal of Marine Science and Technology

Egg abundance of Maurolicus japonicus, a dominant mesopelagic fish in the Japan Sea, was analyzed during 1981-2005 as an index of the spawning-adult abundance. There was a major change in egg abundance around 1988/1989, with negative anomalies occurring during 1981-1988 and positive anomalies occurring from l989. At about the same time (1986/1987), the Tsushima Current shifted from cold to warm conditions. Environmental indices were found to be positively correlated with egg abundance two years later. Our results suggest that the abundance of M. japonicus is affected by climatic regime shifts, and that it could be used as an indicator species.


Purification And Characterization Of Acidic Protease From Aspergillus Oryzae Bcrc 30118, Li-Jung Yin, Ya-Hui Chou, Shann-Tzong Jiang Feb 2013

Purification And Characterization Of Acidic Protease From Aspergillus Oryzae Bcrc 30118, Li-Jung Yin, Ya-Hui Chou, Shann-Tzong Jiang

Journal of Marine Science and Technology

The acidic protease was purified from 4-day cultivation of Aspergillus oryzae BCRC 30118 by DEAE Sephacel and Sephacryl S-200 HR chromatographs. The specific activity, yield and purification fold were 117.62 kU/mg, 15.1% and 6.6, respectively. The molecular weight (M) was 41.0 kDa, while the optimal pH and temperature were 3.0 and 60°C, respectively. It was stable at pH 3.0-6.0 and 4-35°C. However, it was inhibited by Fe2+, Hg2+, Fe3+ and pepstatin A, and slightly by leupeptin and TPCK. According to substrate specificity and inhibitor study, it was a cysteine protease with activation energy of 37.5 kcal/mol. Its Km, Vmax, Kcat …