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Full-Text Articles in Life Sciences
Sperm Decondensation And Male Pronuclear Formation In Bovine Intracytoplasmic Sperm Injection, Lauren Gatenby
Sperm Decondensation And Male Pronuclear Formation In Bovine Intracytoplasmic Sperm Injection, Lauren Gatenby
LSU Master's Theses
This study assessed the effects both DTT and progesterone have on bovine spermatozoa to induce decondensation or the acrosome reaction in order to facilitate male pronuclear formation after ICSI. Sperm prepared by swim-up in a 5 mM concentration of DTT displayed time dependent morphological changes resulting in decondensation over a 6-hour period. An estimated 90% of treated sperm displayed early changes in morphology after the second hour of incubation. Sperm displaying partial decondensation of the nucleus was estimated as 42% by the 3rd hour and increased to 62% by the 5th hour of incubation. Fully decondensed sperm or …
Maturation And Culture Media Effects On In Vitro Bovine Embryo Developmental Competence, Ciara M. Helland
Maturation And Culture Media Effects On In Vitro Bovine Embryo Developmental Competence, Ciara M. Helland
Master's Theses
In vitro produced bovine embryos are critical to the cattle industry. However, these embryos have altered morphology, epigenetics, and metabolism when compared to their in vivo counterparts. The aim of this thesis was to alter maturation and culture media to improve the developmental competence of in vitro bovine embryos. This thesis is comprised of three experiments and one proof of concept study. Each experiment followed the same general layout: oocyte aspiration from Jersey or Holstein ovaries, oocyte maturation for 24 hours, fertilization with bull semen for 24 hours, then embryo culture for 7-8 days in 38.5 °C in 5% O …
Impact Of Ergot Alkaloid And Estradiol 17b On Whole-Body Protein Turnover And Expression Of Mtor Pathway Proteins In Muscle Of Cattle, Taylor Dawn Ferguson
Impact Of Ergot Alkaloid And Estradiol 17b On Whole-Body Protein Turnover And Expression Of Mtor Pathway Proteins In Muscle Of Cattle, Taylor Dawn Ferguson
Theses and Dissertations--Animal and Food Sciences
Beef cattle consuming endophyte infected tall fescue typically exhibit reduced performance in terms of both decreased dry matter intake (DMI) and growth rates. It has been suggested that lower concentrations of circulating IGF-1 (an important stimulator of the mTOR pathway and ultimately protein synthesis), as observed with consumption of ergot alkaloids, contribute to reduced growth rates. The objective of the current study was to determine if fescue-derived alkaloids decrease muscle protein synthesis though inhibitory action on the mTOR pathway via a direct effect on signal proteins, and if these negative effects can be alleviated by implantation with anabolic agents. Thirty-two …