Life Sciences Commons^™

Variation In Personality Among Semi-Wild Myanmar Timber Elephants, Sateesh Venkatesh

Theses and Dissertations

This study examines two personality traits: exploration and neophobia, which could influence human-elephant conflicts. Thirty-one semi-wild elephants were tested over two trials using a custom novel puzzle tube containing three tasks and three rewards. Our studies show that elephants do vary significantly between individuals in both exploration and neophobia.

Evaluating Lignosulfonates Potential As Legume Hay And Silage Preservatives, Angela Yenny Leon-Tinoco

Electronic Theses and Dissertations

The aim was to screen and optimize low-cost lignosulfonates (LST) as legume silage and hay preservatives to decrease losses of DM and nutritive value due to spoilage. In experiment 1, we evaluated the effects of untreated silage (0%), sodium lignosulfonate (NaL) and magnesium lignosulfonate (MgL) applied independently at 0.5, 1, and 1.5 (% w/w, fresh basis) and INO (Pediococcus pentosaceus and Lactobacillus plantarum; 5 and 4 log cfu/fresh alfalfa g, on high moisture alfalfa (Medicago sativa L.) silage nutrient preservation. Data were analyzed as a randomized complete block design (RCBD; 5 blocks) and linear and quadratic polynomial contrasts were used …

Sustainable Production Of Feed For Recirculating Aquaculture Using Black Solider Flies And Microalgae, Patrick Erbland

Electronic Theses and Dissertations

The demand for high-quality, nutritious food continues to increase as human populations grow. As wild fisheries are depleted, aquaculture production is growing to meet the demand for seafood. Sustainable alternatives to wild caught fish meal are increasingly valued for aquaculture feed production. Microalgae and insect larvae are both valuable sources of fatty acids in aquaculture feed. Black soldier fly larvae, Hermetia illucens (L.) are used to convert organic waste streams into insect-based animal feeds. We tested their ability to retain alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) from feeding substrates, which has important implications for their use …

Holistic Monitoring Of Maine Sea Lice (Lepeoptheirus Salmonis, Kroyer, 1837) Sensitivities To Therapies: Developing A Novel Assay To Examine Lice Behavior, Kathryn Liberman

Electronic Theses and Dissertations

Sea lice (Lepeoptheirus salmonis) present significant economic and animal welfare challenges to salmon aquaculture globally. Chemical delousing agents are used in many countries, with each nation eventually reporting sea lice developing reduced sensitivities to treatments. While some countries have in place sea lice sensitivity monitoring programs, that is not the case in Maine, USA. Although chemical delousing agents are not currently used in Maine, they have been used in the past and are currently used in neighboring Canadian salmon farms. Different bay management areas (BMAs) were sampled during different seasons to determine if there is a seasonal or spatial component …

In-Vitro Propagation And Fish Assessments To Inform Restoration Of Dwarf Wedgemussel (Alasmidonta Heterodon), Jennifer Ryan

Masters Theses

The dwarf wedgemussel (Alasmidonta heterodon) is a federally endangered freshwater mussel that once ranged from New Brunswick to North Carolina, but now only exists in isolated populations throughout its diminished range. Laboratory propagation in conjunction with augmentation or reintroduction is considered a critical component of dwarf wedgemussel restoration. My thesis aimed to I) develop in-vitro propagation techniques including methods to minimize microbial contamination for two Alasmidonta species and II) compile and assess critical fish assemblage information at existing dwarf wedgemussel presence and absence locations to inform future restoration activities. For the first objective (Chapter 2) I assessed three …

New England’S Underutilized Seafood Species: Defining And Exploring Marketplace Potential In A Changing Climate, Amanda Davis

Masters Theses

New England’s seafood industry has been searching for opportunities to diversify their landings and build resilience as it faces socio-economic challenges from a changing climate. Developing markets for underutilized species is one way the New England community could help their seafood industry build resilience. This thesis identified New England’s underutilized fish species and explored their marketplace potential by examining their availability in a changing climate, current availability to consumers, and consumers’ responses. In Chapter I, I account how New England’s seafood preferences have changed over time. In Chapter II, I identify New England’s seven underutilized seafood species: 1) Acadian redfish …

Wildlife Rehabilitation Datasets As An Underutilized Resource To Understand Avian Threats, Mortality, And Mitigation Opportunities, Michelle M. Duffy

Electronic Theses and Dissertations

Wildlife rehabilitation centers collect large datasets that focus on medical care, yet they also collect information more broadly relevant to wildlife conservation. The goal of this study was to demonstrate the potential for these datasets to be used in conservation science to better understand avian threats, mortality, and mitigation opportunities. We quantified the causes of bird admissions to rehabilitation centers within the Northeast and Midwest United States, the mortality rates during rehabilitation by admission cause, and the proportion of anthropogenic-caused admissions. Additionally, we related human population and development metrics to the number of bird admissions to better understand geographic bias …

The Effect Of Holding Time Of Whole Mare Blood On Concentration Of Progesterone, Clarissa Menefee

Electronic Theses and Dissertations

The objective of this study was to evaluate if holding time had an effect on mare whole blood progesterone levels. This research was completed using 12 mares, with blood being collected on multiple cycles to get a total of 28 separate blood collections. At each collection 5 vials of blood were taken for different time treatments of 0, 12, 24, and 48 hours. The 0 hour was centrifuged within 30 mins of collection and the other 4 were refrigerated at 4℃ until the specific time treatment was finished. After they were centrifuged all the samples were stored in the freezer …

Prevalence Of Snake Fungal Disease Caused By Ophidiomyces Ophiodiicola In East Texas, Alan Lizarraga

Biology Theses

Fungal pathogens and resultant disease are credited with the decline of many species across all branches of the tree of life. Fungal diseases such as Snake Fungal Disease (SFD), primarily caused by the fungus Ophidiomyces ophiodiicola (Oo), have been strongly associated with the cause of the decrease/disappearance of many snake populations in North America. To date SFD in the wild has been described as far southwest as central Louisiana. Due to similar conditions and the proximity of East Texas to central Louisiana, a survey of local snake populations provided crucial information about the spread and presence of this emerging pathogen. …

Residency, Diel Movement, And Tidal Patterns Of Large Juvenile Bull Sharks (Carcharhinus Leucas) In Winyah Bay, Sc, Jeremy Lee Arnt

Electronic Theses and Dissertations

No abstract provided.

The Fear Of Reptiles And How To Change It, Maxwell Lyman

Honors Projects

Reptile fear is prominent across many cultures. Anti-reptilian attitudes can lead to anti-conservation attitudes towards reptiles. Person-animal interaction has been shown to decrease fear desensitization and increase positive attitudes towards "unpopular" animals. My project demonstrates the effectiveness of live animal presentation in dispelling negative attitudes of reptiles. However, due to the sample size of the project, further research is highly suggested.

Hormonal Responses To Seasonal Thermal And Ecological Stressors In Japanese Macaques (Macaca Fuscata), Lilianne Nelson

Masters Theses

Relative to most primate species, Japanese macaques (Macaca fuscata) experience extreme seasonal variation in thermal and ecological stressors that can impact energetic demands. To cope with these environmental changes, levels of metabolic hormones, such as cortisol and triiodothyronine, fluctuate to facilitate energetic adjustments. While previous research in primates has investigated thermal and ecological stressors individually, a combined assessment of these stressors alongside hormone levels can provide a more holistic understanding of the relationship between a primate’s thermoregulation, energetic balance, and stress. The goals of this study were to determine the effects of season, temperature and ecological stress on …

Evaluation Of Sperm Cryopreservation For Yellow Perch (Perca Flavescens) Broodstock Management, Sonya Ponzi

Theses and Dissertations

The demand for yellow perch in the Great Lakes region has withstood time despite declining yellow perch populations in the Great Lakes. Today aquaculture can aid in meeting the demand. However, the technologies such as cryopreservation which would allow for large-scale, year-round broodstock operations is underdeveloped. Cryopreservation of fish gametes has many practical applications for use in aquaculture including assistance in asynchronized spawns, preservation of genetic resources with known pedigree and quality, and simplification of gamete transportation. This thesis evaluated the most current yellow perch semen cryopreservation protocol to determine whether fresh and cryopreserved semen differ in their ability to …

Examining Pathogenesis And Preventatives In Spontaneous And Staphylococcus-Induced Bacterial Chondronecrosis With Osteomyelitis In Broilers, Nnamdi Simon Ekesi

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis is the most important cause of lameness in broilers. This is important to poultry production, as it poses animal welfare issues, and causes a significant loss in revenue. The remediation of this disease requires the study of its etiology with fitting models and evaluating preventatives. The research reported herein covers genomic virulence analysis of BCO isolates, mainly Staphylococcus aureus, and Escherichia coli retrieved from lame birds. We found that S. aureus isolates were closest to chicken strains in Europe but may have been in the Arkansas area for a decade. Phylogenomics suggest our S. aureus is …

The Surveillance Of Gastrointestinal Parasitic Nematodes Of Northwest Arkansas Dairy Cattle Using Traditional And Genetic Parasitological Identification Procedures, Eva M. Wray

Graduate Theses and Dissertations

Traditional and genetic parasitological identification procedures were compared using naturaland artificial nematode parasite infections in Holstein steer calves. The traditional parasitological procedures measured fecal egg counts, coprocultures with subsequent larval collection and adult nematodes collected at necropsy. The genetic identification procedures measured ITS-2 sequences extracted from different stages of nematode development: raw feces, concentrated nematode eggs, third stage larvae and adults. The primary nematodes observed were Cooperia oncophora, Cooperia punctata and Ostertagia ostertagi. The traditional techniques were not significantly different from one another, while the genetic sequencing showed variation amongst the different procedures. The raw feces sequences showed the most …

Investigating The Potential Of Microbial Metabolites And Mtor Signaling On Chicken Necrotic Enteritis, Mohit Bansal

Graduate Theses and Dissertations

Necrotic enteritis (NE), caused by C. perfringens and coccidiosis, is responsible for substantial economic loss annually. In the first chapter, we investigated the effect of secondary bile acid on necrotic enteritis. Day-old broiler chicks were randomly assigned to 5 groups of diets supplemented with 0 (basal diet), 0.8, 1.0, and 1.5 g/kg (on top of basal diet) deoxycholic acid (DCA). The birds were challenged with Eimeria maxima (20,000 oocysts/bird) at d 18 and C. perfringens (109CFU/bird/day) at d 23 and d 24 to induce NE. Birds were sacrificed at d 26, and ileal tissue and digesta samples were collected. DCA …

Effects Of Commercial Feed Additives On Growth Performance And Gut Microbiota Of Nursery Pigs, Xiaoyuan Wei

Graduate Theses and Dissertations

To secure animal welfare and maximum production, feed additives are often added to nursery pig diets to increase nutrition digestibility, enhance intestinal health, and prevent or mitigate disease, with the ultimate goal of improving growth performance. This thesis performed a longitudinal analysis using next-generation sequencing to investigate the dynamic changes of gut microbiota in weaned pigs fed commercial feed additives. Chapter II was used to evaluate the effects of organic acid mixture on growth performance and gut microbiota of weaning pigs. This study demonstrated that the inclusion of 0.035% (SBA0.035) or 0.070% (SBA0.070) sodium butyrate in a diet containing 0.5% …

Rumen Fermentation Responses To Purified Palmitic, Stearic, Or Oleic Fatty Acids And The Impact Of A Palmitic Acid-Enriched Supplement On Animal Performance, Austin Paul Sears

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Our research examined the responses of palmitic, stearic and oleic fatty acids on neutral detergent fiber (NDF) digestibility and rumen fermentation and the impact of a palmitic acid-enriched supplement on animal performance. In the first research chapter, we evaluated the effect of purified palmitic, stearic, and oleic fatty acids on NDF digestibility and rumen fermentation. Palmitic acid increased NDF digestibility and total production of volatile fatty acids (VFA) compared with oleic acid. Bacterial fatty acid composition was altered by palmitic acid, increasing anteiso-C15:0, C18:2n6 and C18:3n3 fatty acids compared with oleic acid. Compared with control, …

Effects Of Premortem Stress On Heat Shock Protein Abundance, Oxidation, And Color In The Longissimus Lumborum Of Holstein Steers Following Harvest, Reganne K. Briggs

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Consumers consider tenderness and steak color to be two important attributes associated with meat quality. However, inconsistencies in both tenderness and steak color remain despite similar production practices of beef cattle. Stressful events before harvest may impact meat quality by initiating certain pathways such as abundance of heat shock proteins (HSP) and oxidation within the skeletal muscle. Heat shock proteins have been associated with tenderness while oxidation may affect steak color and flavor. In this study, 40 Holstein steers were administered adrenocorticotrophic hormone (ACTH) to mimic a stressful event before harvest. Animals were harvested at different times following the ACTH …

Novel Polymorphisms Of Zrsr2 And Gpm6b Gene Homologs And Their Use In Sex Identification Of Bovine And Porcine Species, Evan K. Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Accurate and cost-effective PCR based sex identification is important in animal production because it gives producers the ability to determine the sex of embryos prior to transfer, saving time and money. The most efficient PCR sex identification assays work by using a single primer pair to amplify a specific target region located on the Y-chromosome and a second, separate target region on the X-chromosome.

This thesis reports the design of two novel assays. The first assay was designed to target the Zinc finger CCCH-type, RNA binding motif and serine/arginine rich 2 (ZRSR2) gene found on the X-chromosome and its Y-chromosome …

Demographics, Accuracy, And Impact Of Feed Laboratories In The United States, Jerald H. Severe

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Feed analysis is very important to modern society. In the United States feed analysis is used to optimize production of food animals. Feed analysis is also used as a tool to place value on crops. As important as feed analysis is to society, little research has been done that describes which feed laboratories are the most popular and why people use them. It has been thought by some patrons that different results from the same feed sample are obtained by different laboratories. Is this true? If so, what is the effect on those that use feed laboratories to produce animals, …

Evaluation Of Selected Bacillus Direct-Fed Microbial Candidates In Reduced Energy Diets On Live Performance, Carcass Characteristics, And Foot Pad Dermatitis, Kyle Teague

Graduate Theses and Dissertations

Bacillus spp. are ubiquitous, Gram-positive, spore forming bacteria that are commonly recovered from the environment and gastrointestinal tract (GIT) of poultry. These spores are capable of withstanding harsh condition such as feed pelletization, which facilitates inclusion in poultry feeds. Once ingested by the bird, spores germinate into metabolically active vegetative cells which can produce extracellular enzymes which can hydrolyze otherwise indigestible components of the feed. Soybean meal (SBM) is the gold standard vegetable protein source for non-ruminant animals worldwide and is included in practically all poultry diets in the United States at levels ranging from 10 to more than 30%. …

Microplastic Abundance, Distribution And Impacts On Sargassum-Associated Juvenile Fishes In The Gulf Of Mexico, Olivia Lestrade

Master's Theses

Microplastics are a concern in marine environments because they are highly durable, ubiquitous, and can be mistaken for food and ingested by small organisms. Pelagic Sargassum, an important habitat for larval and juvenile stages of many fish species, is found in large surface aggregations, and may provide complex structure in which microplastics become trapped. This could lead to greater risk of microplastic ingestion by fish early life stages associated with Sargassum habitats. To better understand the impacts of microplastics within Sargassum communities, this study examined 1) microplastic concentrations and ingestion by juvenile fishes associated with Sargassum; 2) the microbial …

Assessing The Nursery-Role Function Of Pelagic Sargassum For Juvenile Fishes In The Northern Gulf Of Mexico, Courtney Stachowiak

Master's Theses

Sargassum, a genus of holopelagic brown algae, floats at the ocean’s surface using air-filled bladders and forms a complex comprised of two species, S. natans and S. fluitans. Oceanic processes (e.g., Langmuir circulation, etc.) aggregate Sargassum into mats and weedlines, and primarily distribute the algal complex throughout the North Atlantic Ocean and the Gulf of Mexico (GOM). These floating habitats provide shelter and feeding opportunities for a diverse community of invertebrates and fishes. Sargassum is a presumed nursery habitat for juvenile stages of commercially- and recreationally-targeted fishery species. In this study, I estimated the standardized abundances of juvenile …

Determining The Trophic Role Of Red Snapper (Lutjanus Campechanus) In Mississippi State Waters Using Stomach Content And Stable Isotope Analysis, Branden Kohler

Master's Theses

The goal of this study was to determine the diet composition, trophic position and ecological role of red snapper (Lutjanus campechanus) in Mississippi state waters utilizing stable isotopes (δ¹³C and δ¹⁵N) and stomach content analysis. Stable isotope analysis of fish and their prey can provide information on species-specific basal resource utilization, diet composition and trophic position which can improve food web models and inform fisheries management decisions. Particulate organic matter (POM), the presumed base of the food web, red snapper muscle tissue, and red snapper stomach contents were collected from 25 sites in 2016 and …

Effects Of Long-Term Variation In Temperature On Reproductive Phenology In A Population Of Eastern Bluebirds (Sialia Sialis), Paul Pleiman

Graduate Theses and Dissertations

This study investigates the relationship between multiple temperature variables, to include annual and pre-lay date temperatures with first-egg and mean first-egg lay dates of the eastern bluebird at the Warner Parks in Nashville, Tennessee, USA. Data is collected by citizen scientists for the Eastern Bluebird Nesting Box Project while visiting artificial nest boxes throughout the park and recording observations made during the breeding season. Temperature data is retrieved from the Northwest Alliance for Computational Science and Engineering’s Parameter-elevation Regressions on Independent Slopes Model (PRISM) Climate Group, based at Oregon State University. The analyses showed no correlation between annual or pre-lay …

Evaluation Of Different Ensiling Methods On Storage And Feeding Value Of The Residual Material From Edamame Soybean Processing, Ellen Herring

Graduate Theses and Dissertations

Use of organic waste material by ruminant animals from food processing operations potentially reduces costs and reduces environmental issues from disposal of these residues. Therefore, 2 experiments were conducted to evaluate the storage and feeding value of residual from edamame soybean processing for ruminant animals. Two types of waste streams, waste during harvest time and waste from processing stored material, were ensiled (on a laboratory scale) using various methods and effects on post-ensiling nutritive value were examined. Material from both waste streams were ensiled either without wilting or after wilting; each moisture level was ensiled with and without an inoculant. …

Biogeography Of The Bovine Respiratory Microbiome And Its Relationship With Bovine Respiratory Disease, Jianmin Chai

Graduate Theses and Dissertations

Bovine respiratory disease (BRD) is the most common and costly disease in the beef cattle industry, leading to high morbidity, mortality and huge economic loss. Despite the recent advances in vaccination and antimicrobial techniques, no significant health-improved outcomes have developed. Due to a deep investigation of the microbiome, respiratory microbiotas are known to have important roles for host health and disease. However, BRD specific pathogens have not yet been identified since they are found in both healthy and diseased animals. A systemic and comprehensive study of the biogeography of the bovine respiratory microbiome and its relationship with BRD is lacking …

The Use Of Inorganic And Organic Acids As Short Duration Antimicrobial Dips On Mitigating Pathogens Present On Commercial Chicken During Peri-Harvest, Dana Kristen Dittoe

Graduate Theses and Dissertations

With poultry being the most consumed meat in the United States, poultry processors must provide consumers with safe, wholesome products. As a consequence, poultry processors are faced with the challenge of reducing the presence of foodborne pathogens such as Salmonella spp. and Campylobacter jejuni among raw poultry products. Though multi-hurdle approaches using antimicrobials are placed throughout processing to reduce these pathogens, Salmonella and C. jejuni still persist among raw poultry. Thus, it was the objective of the current dissertation to investigate various antimicrobials, organic and inorganic acids, as short duration dips and sprays as means to reduce common pathogens (Salmonella, …

Predicting Voluntary Forage Intake Of Supplemented Beef Cattle, Connor Biehler

Masters Theses

A major priority of beef cattle production is to meet animal nutrient requirements in order to achieve a desired level of productivity. Accurately predicting voluntary forage intake (VFI) is necessary to accurately predict the total nutrient intake of grazing or forage-fed beef cattle that are also supplemented with other sources of nutrients. Therefore, the objectives of this experiment were to utilize data from published literature to 1) identify factors that explain variation in VFI, and 2) develop and validate one or more mathematical models that predict VFI or total nutrient intake of grazing or forage-fed and supplemented beef cattle. A …