Life Sciences Commons^™

Unraveling The Role Of Lipid Droplets And Perilipin 2 In Bovine Luteal Cells, Michele R. Plewes, Heather A. Talbott, Micah B. Schott, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

Steroidogenic tissues contain cytosolic lipid droplets that are important for steroidogenesis. Perilipin 2 (PLIN2), a structural coat protein located on the surface of lipid droplets in mammalian cells, plays a crucial role in regulating lipid droplet formation and contributing to various cellular processes such as lipid storage and energy homeostasis. Herein, we examine the role that PLIN2 plays in regulating progesterone synthesis in the bovine corpus luteum. Utilizing gene array databases and Western blotting, we have delineated the expression pattern of PLIN2 throughout the follicular to luteal transition. Our findings reveal the presence of PLIN2 in both ovarian follicular and …

Temporal And Spatial Influences On Fawn Summer Survival In Pronghorn Populations: Management Implications From Noninvasive Monitoring, Cole A. Bleke, Eric M. Gese, Juan J. Villalba, Shane B. Roberts, Susannah S. French

Wildland Resources Student Research

Monitoring vital rates allows managers to estimate trends in growth rates of ungulate populations. However, connecting the influence of nutrition on ungulate demography is challenging. Noninvasive sampling offers a low-cost, low-effort alternative for measuring nutritional indices, allowing for an increased understanding of the mechanistic relationships between environmental factors, nutrition, and specific population vital rates. We examined the temporal influence of intrinsic and extrinsic factors on pronghorn (Antilocapra americana) fawn recruitment. We collected fresh fecal samples from adult female pronghorn in five subpopulations spanning three sampling periods associated with critical maternal life-history stages (late gestation, early lactation, breeding season) …

Heavy Steers And Heifers Run At Low Stocking Rates Enhance Drought Resilience In A Pastoral Region Of Australia, Christophe D'Abbadie, Ross Kingwell, Philip E. Vercoe, Bradley Plunkett, Alan Peggs

Animal Production and Livestock Research Articles

CONTEXT

The semi-arid mulga lands of the Southern Rangelands of Western Australia experience frequent droughts. Maintaining a profitable animal grazing enterprise whilst preserving rangeland condition requires selecting an appropriate stocking rate and herd structure.

OBJECTIVE

We construct and apply bioeconomic models of beef enterprises for the semi-arid Southern Rangelands of Western Australia, to assess the profitability and relative riskiness of three different livestock production systems and three different stocking rates when exposed to different frequencies of drought.

METHODS

We construct bio-economic models of herd structures that separately focus on producing either: (i) 200 kg heifers and 210 kg steers (ii) …

Eviction-Driven Infanticide And Sexually Selected Adoption And Infanticide In A Neotropical Parrot, Steven R. Beissinger, Karl S. Berg

School of Integrative Biological and Chemical Sciences Faculty Publications and Presentations

Significance

Infanticide and adoption are puzzling forms of sexual conflict and cooperation, respectively. However, both may be explained by sexual selection, where an individual later reproduces with the parent whose offspring it killed or adopted. While sexually selected infanticide is well known, evidence for sexually selected adoption is anecdotal. Our long-term study of a parrotlet in Venezuela found infanticide attacks were mostly enacted by nonbreeding pairs attempting to evict parents from their nests to usurp the cavity. Infanticide attacks occurred less often at nests where a parent died, and adoption by stepparents was as common as infanticide. Becoming an adoptive …

Using Annual Forages To Replace Declining Cool-Season Grass Pasture Availability, Jonathon Matthew Jenkins

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

As land area in grain crop production increases in the US Midwest, perennial pasture availability decreases but the demand increases. Matching the seasonal growth patterns of C₃ and C₄ plant species to grow forages that are complementary allows producers to fill production gaps in otherwise monoculture systems and possibly increase overall productivity. One way to fill the forage production gap of C₃ grass dominated pastures is by producing a warm-season, annual forage in a separate field. However, with continual reductions in land availability, this may not be an option. Using two studies, our objectives were to improve …

Adding Parasite Resistance To A Hair Sheep Breeding Objective, Bob Forbes

Department of Animal Science: Dissertations, Theses, and Student Research

The U.S. Hair Sheep Index was designed to increase total weight of lamb weaned per ewe lambing (TW) using estimated breeding values (EBV) for direct (DWWT) and maternal (MWWT) weaning weight, and number of lambs born (NLB) and weaned (NLW), as selection criteria. Producers are interested in adding post-weaning fecal egg count EBV (PFEC), an indicator of parasite resistance, to the index to achieve genetic change in both TW and PFEC.

First, the effects of adding PFEC alongside TW to the breeding objective of indexes that used either phenotypes or EBV as selection criteria were studied. Since MWWT is …

Utilization Of The NuTrack System To Objectively Evaluate Changes In Active And Passive Behaviors Of Group-Housed Nursery Pigs Exposed To An Endotoxin Challenge, Aaron Holliday

Department of Animal Science: Dissertations, Theses, and Student Research

This study aimed to evaluate the changes in activity of group-housed, newly weaned pigs challenged with lipopolysaccharide (LPS). At weaning, pigs (n = 192, 5.73 ± 1.8 kg) were stratified by sex, litter, and body weight (BW) and randomly assigned to one of three treatments (16 pigs/pen, 4 pens/treatment): 1) Saline-injected (SAL), 2) 50% challenged [50%-LPS) – only half of the pigs in a pen (8 pigs) were challenged with LPS and 3) 100% challenged (100%-LPS) – all pigs in a pen were challenged with LPS. Pigs in the SAL and 32 in the 50%-LPS treatment received a 3.0 …

Influence Of Beef Carcass Weight On Rate Of Desmin Proteolysis In Four Muscles, Clay J. Carlson, Christina E. Bakker, Keith R. Underwood, Amanda D. Blair, Judson K. Grubbs

Animal Science Research Report 2024

No abstract provided.

Effects Of Peri-Castration Pain Management Strategies On Feed And Water Intake, Performance, And Blood Cortisol Levels Of Weaned Beef Steers, Andrea K. Brandner, Cody L. Wright, Ana Clara B. Menezes

Animal Science Research Report 2024

No abstract provided.

Influence Of Vegetative Diversity During The Stocker Phase On Carcass Characteristics And Economic Returns Of Beef Steers, Sydni Borders, Jameson Brennan, Christina Bakker, Keith Underwood, Kyle Grubbs, John Jaeger, Ken Olson, Amanda Blair

Animal Science Research Report 2024

No abstract provided.

Influence Of Finishing Systems On Carcass Characteristics, Composition, And Fatty Acid Profile Of Bison Bulls, Clay J. Newton, Lydia M. O'Sullivan, Keith R. Underwood, Judson K. Grubbs, Christina E. Bakker, Kristi M. Cammack, Thu Dinh, Carter Kruse, Amanda D. Blair

Animal Science Research Report 2024

No abstract provided.

Utilization Of Camera Grading Technology For Bison Carcass Characteristics, Christina Bakker, Jessica Sparks, Keith Underwood, Kyle Grubbs, Amanda D. Blair

Animal Science Research Report 2024

The objective of this study was to evaluate the ability of beef camera grading technology to assess characteristics of bison carcasses.

Comparative Accumulation And Effects Of Microplastics And Microplastic-Associated Pcb-153 In The White Hard Clam (Meretrix Lyrata) And Giant River Prawn (Macrobrachium Rosenbergii) Following Chronic Exposure, Bao-Son Trinh, Lien Thi Le, Loc Minh Tran, Gunther Rosen, Robert C. Hale

VIMS Articles

Global environmental abundance of microplastics (MPs) is increasing. MPs may sorb hydrophobic organic contaminants (HOCs), accumulate in and cause deleterious effects on exposed organisms. This study investigated and compared the accumulation and effects of MPs and MP-associated PCB in the two indigenous aquatic organisms in Viet Nam, the white hard clams, Meretrix lyrata, and the giant river prawns, Macrobrachium rosenbergii. The test organisms were exposed to either polyethylene microbeads (PEMBs), waterborne polychlorinated biphenyl 153 (PCB-153), or PEMB-associated PCB-153 (PEMB-PCB) over 28 days. Organismal MP accumulation, survival, and weight gain were examined at various sampling intervals. In general, MP …

Comparing The Effectiveness Of Different Forms Of Vitamin E Supplementation (Liquid Vs. Powder) On Serum Alpha-Tocopherol Concentrations In Developing Horses, Emilia Fong-Gallagher

Honors Scholar Theses

Vitamin E is an essential antioxidant in equines that has a significant role in the nutritional requirements of horses to keep bodily systems functioning at optimal levels. Vitamin E deficiency is often associated with serious incurable neuromuscular diseases such as equine motor neuron disease and equine protozoal myeloencephalitis. Many horses stabled in urban areas are often deficient due to the lack of adequate grazing time as their natural source of vitamin E is from grass, and therefore should receive supplementation. This study evaluated the effectiveness of different forms of Elevate vitamin E supplement on the serum alpha-tocopherol concentrations in fourteen …

West Coast Rock Lobster Resource Harvest Strategy, Department Of Primary Industries And Regional Development, Western Australia

Fisheries management papers

Harvest strategies for Western Australia’s (WA) aquatic resources are formal documents developed by the Department of Primary Industries and Regional Development (DPIRD, the Department) to support decision-making processes that ensure the outcomes are consistent with the principles of Ecologically Sustainable Development (ESD; Fletcher 2002a) and Ecosystem Based Fisheries Management (EBFM; Fletcher et al. 2012). Harvest strategies are a key component of all contemporary fishery management systems and a requirement for certification under the Marine Stewardship Council (MSC). The objectives of ESD are reflected in the objectives of the Fish Resources Management Act 1994 (FRMA) and the Aquatic Resources Management Act …

Feeding Dried Distillers Grains With Solubles To Lactating Dairy Cattle: Whole Animal Energy Utilization And Manure Biogas Production, Grant Michael Fincham

Department of Animal Science: Dissertations, Theses, and Student Research

When DDGS are fed to lactating dairy cattle, the observed response in energy supply, milk production, and methane production varies among studies. One potential reason for these observed discrepancies is the nature of the diet formulation itself. Furthermore, little research has been conducted to examine how dietary changes can affect dairy cattle manure composition and methane production in an anaerobic manure digester. In order to gain a better understanding of these topics, we conducted two experiments. The first experiment was conducted to test how the manipulation of lactating dairy cattle diets affects energy utilization, milk production, methane production, and manure …

Design Of Small-Scale Milk Processing Facility, Luke Bond, Robert Stenzel, Ry Steffen, John Van Nieuwenhuyse, Rossana Villa-Rojas, Terry Howell Jr., Tami M. Brown-Brandl, Forrest Kievit

Department of Biological Systems Engineering: Masters Project Reports

This project was intended to investigate the feasibility of a proposed modular dairy processing facility collocated with a small dairy on the University of Nebraska–Lincoln’s Innovation Campus. Priority was placed upon cost savings for equipment to be used in processing. Process flow diagrams, floor plans, production schedules, risk assessments, and plans for wastewater treatment were all developed to address the needs of the plant should it be built. A Monte Carlo simulation was conducted to evaluate the timeline for breaking even financially, resulting in the potential for a payback period of 4.5 years. The 4.5-year payback period was based on …

The Impact Of A Pgym Variant On Myophosphorylase Deficiency In Red Angus Composite Cattle And Changes In The Skeletal Muscle Transcriptome Due To The Intramuscular Administration Of Lidocaine In Wether Lambs, Mackenzie Christin Batt

School of Biological Sciences: Dissertations, Theses, and Student Research

Project 1 focused on eight calves in a Nebraska herd (composite Simmental, Red Angus, Gelbvieh) that displayed exercise intolerance during forced activity. Available sire pedigrees contained a paternal ancestor within 2-4 generations in all affected calves. Pedigrees of the calves’ dams were unavailable, however, the cows were ranch-raised and retained from prior breeding seasons, where bulls used for breeding occasionally had a common ancestor. Therefore, it was hypothesized that a de novo autosomal recessive variant was causative of exercise intolerance in these calves. A genome-wide association analysis followed by whole-genome sequencing led to the identification of a variant in the …

Phenotypic Plasticity In Pigmentation: Investigating Coloration Patterns In Anablepsoides Hartii Population Responses To Environmental Variation, Jasdeep Kaur

2024 Spring Honors Capstone Projects

In this study, we investigated the color-changing capabilities of Trinidadian killifish, Anablepsoides hartii, through exposure to varied color conditions over defined periods of time. Our experiment aims to understand adaptive responses when killifish are subjected to different color backgrounds, exploring their mechanisms to change color. By subjecting the species to different environmental color variations, we sought to discern color change patterns, which could indicate adaptive responses related to camouflage, stress, behavior clues, and physiological adjustments. Carefully controlling environmental factors, we monitored and documented the fishes’ color changes, aiming to provide insights into the interplay between fish’s developmental plasticity and its …

Artificial Insemination And Fetal Programming In Beef Production Systems, Dempster Christenson

Department of Animal Science: Dissertations, Theses, and Student Research

Pregnancy to artificial insemination and late gestation fetal programming of the next calf crop are central to genetic development, reproductive longevity, calf crop productivity, and efficiency of cow/calf ranching. The purposes of this research are to find methods to improve pregnancy rate to artificial insemination, the growth and reproductive health of pregnant heifers, and the productivity of their offspring. In the first of four studies we found that extending the period of progesterone administration in an estrus synchronization protocol did not significantly hasten estrus response or increase pregnancy rate to artificial insemination, but timing of estrus within the melengestrol acetate …

Impact Of Strategic Postweaning Supplementation On March- And May-Born Yearling Range Heifer And Calf Growth And Performance In The Nebraska Sandhills, Josie Nicole Crouch

Department of Animal Science: Dissertations, Theses, and Student Research

Three experiments were conducted at the Gudmundsen Sandhills laboratory near Whitman, NE. Experiment 1, a retrospective regression analysis, evaluated the impact of heifer percentage of mature body weight (BW) at breeding on heifer performance, subsequent pregnancy rates, and calf production. Overall pregnancy rates were greater (P < 0.01) for heifers at 60 to 70% of mature BW than 50 to 55%, with no differences in pregnancy rates (P ³ 0.15) after 2-years of age. Heifers at 50 to 60% of mature BW had a greater (P =0.05) proportion give birth in the first 21-d. A higher percentage of mature BW at breeding resulted in greater (P < 0.01) BW at birth and weaning for offspring.

Experiments 2 and 3 were 3-year studies that evaluated the impact of …

Sheep And Goat Industry Funding Scheme Annual Report 2022/2023, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

The Sheep and Goat Industry Funding Scheme (IFS) has been operating since 2010 to address biosecurity threats relevant to the Western Australian (WA) sheep and goat industry. The Scheme was established by regulation under the Biosecurity and Agriculture Management Act 2007 to enable sheep and goat producers to identify pest and disease priorities at a whole-of-industry level and raise funds for activities to address these priorities.

There are currently three Industry Funding Schemes in operation. In addition to the Sheep and Goat IFS, there is a Cattle IFS and a Grains, Seeds and Hay IFS. The three IFSs operate in …

Does Urbanization Surrounding Stopping Sites Affect Migratory Behavior In American Woodcock (Scolopax Minor)?, Zoe Pavlik

Honors College

Urban landscapes may influence bird species in complex ways, with some species exploiting urban environments, others adapting to them, and others avoiding them. Migratory birds encounter urbanization not just during breeding and overwintering, but also at stopping sites during migration. Migration routes of American Woodcock (Scolopax minor), a bird species experiencing long term population declines, follow the east coast of the United States, including the major urbanized areas of the eastern seaboard. I explored the effects of urbanization around stopping sites on Woodcock migratory behavior using the percentage of impervious surfaces within a buffer surrounding the site as a measure …

Winging It Through Unprecedented Times: A Study Of Avian Admissions To Wildlife Rehabilitation Centers In The Eastern United States Amidst The Covid-19 Pandemic, Thea Tengstrom

Honors College

The onset of the COVID-19 pandemic in 2020 has brought new research that examines the many ways in which humans impact wildlife. Wildlife rehabilitation is a tool that attempts to mitigate some negative effects of anthropogenic activity (domestic cats, window strikes), through specialized care to aid injured and orphaned animals. This study sought to investigate if the ‘Anthropause’, which saw a massive change in human behavior, demonstrated an influence on wild bird admissions to wildlife rehabilitation centers. Twelve centers were selected including both urban and non-urban centers throughout the eastern United States. Bird admission data between 2018 and 2021 were …

Evaluating The Effects Of Parasite Infections On Reproductive Ability In Maine Moose, Isabella Costa

Honors College

Despite conservation efforts, moose have experienced increasing mortality rates. Winter ticks are known to cause anemia and lower reproductive potential in moose. Moreover, a genus of bacteria, Anaplasma spp., is known to cause a reduction in reproductive efforts in other animals, such as cattle. Because both winter ticks and Anaplasma spp. may affect female reproductive ability, the overall goal of this research was to determine if Anaplasma bacterial infections could be impacting female moose reproduction within the state of Maine. To address this, samples and biological data from moose were collected during hunter harvest, in collaboration with hunters and the …

Ecological, Molecular, And Electron Microscopical Investigations Of The Distribution Of Shell-Boring Polychaetes Along The Coast Of Maine, Emma Tomasetti

Honors College

Blister worms are polychaete worms that live in various calcareous materials such as dead shells, red algae, limestone, and cinder blocks, along with cultured shellfish, such as oysters or sea scallops. These worms can be considered a pest due to their nature to burrow into their host’s substrate and create blisters that then fill with mud and detritus. In chapter 1, I worked closely with an oyster farm looking at the reproductive status of one species of blister worm, P. websteri, and the efficacy of cold storage as a method of ridding oysters of P. websteri. For chapter 2, I …

Temperature And Energy Interactions In Small Tropical Mammals, Abe Grunwald

Honors College

The study of mammalian energetics focuses on the complex connections between metabolic rate, body temperature, thermoregulation, and much more. Knowledge of mammalian energetics is skewed towards temperate endotherms, making assumptions about energetics biased towards the more studied temperate species. However, tropical endotherms live in environments that are quite different from temperate endotherms, resulting in differences in heat exchange between the endotherm and the environment. To understand how the different dynamic created between ambient temperature and thermoregulation impacts energetics in the tropics, I compare two species of tropical mammals, one with nighttime activity, the western tarsier (Cephalopachus bancanus), and one with …

Virginia Game Fish Tagging Program: 2023 Annual Report, Susanna Musick, Lewis Gillingham

Reports

Through 2023, the Virginia Game Fish Tagging Program (VGFTP) has maintained a 28-year database of records for tagged and recaptured fish. The program is a cooperative project of the Virginia Saltwater Fishing Tournament (VSFT) under the Virginia Marine Resources Commission-(VMRC) and the Virginia InsƟtute of Marine Science (VIMS) of William & Mary (under the VIMS Marine Advisory Program).

The main goal of the program is to train and maintain an experienced group of anglers who volunteer their Ɵme to properly tag and release fishes. Data on tagged and recaptured fish are summarized in annual re- ports and are accessible on …

Conservation Targeting: Models And Policy For Climate Resilience Of Coastal Habitat And Heritage Resources In Virginia’S Coastal Zone, Pamela Mason, Robert Isdell, Christine Tombleson, Rob Evans, David N. Bucklin, Anne C. Chazal, Dan Hannon, Jason F. Bulluck, Joseph T. Weber, Elizabeth Andrews, Gray Montrose

Reports

In response to accelerating sea level rise and its anticipated impacts on coastal habitats and species, we developed spatial models and maps of shoreline habitat distributions in Coastal Virginia to the year 2100. This report is a culmination of a three-year effort to develop and serve geospatial data to inform land use decision making within the context of projected habitat and current elemental occurrences to promote habitat and species persistence and resilience. The project was a collaboration of the Center for Coastal Resources Management (CCRM) at the Virginia Institute of Marine Science (VIMS the Virginia Department of Conservation and Recreation, …

Estimation Of Juvenile Striped Bass Relative Abundance In The Virginia Portion Of Chesapeake Bay Annual Progress Report: 2023 - 2024, Jack R. Buchanan, Mary C. Fabrizio, Troy D. Tuckey

Reports

The 2023 juvenile Striped Bass abundance index was 4.26 and was significantly lower than the reference mean of 7.77 for the period 1980 to 2009. Abundance indices were below average in the James and Rappahannock rivers and average in the York River in 2023 compared with the river-specific reference means (1980-2009). Relatively low catches of young‐of‐the‐year Striped Bass from sites upriver and downriver of core nursery areas suggest juvenile Striped Bass largely remained within core nursery areas in 2023.

Indices of abundance were calculated for three additional economically and recreationally important fishes in Virginia waters. Juvenile White Perch abundance indices …