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Articles 1 - 12 of 12
Full-Text Articles in Life Sciences
Continuous In Vitro Culture Of The Carpet Shell Clam Tapes Decussatus Protozoan Parasite Perkinsus Atlanticus, Sm Casas, Jf La Peyre, Kimberly S. Reece, C Azevedo, A Villalba
Continuous In Vitro Culture Of The Carpet Shell Clam Tapes Decussatus Protozoan Parasite Perkinsus Atlanticus, Sm Casas, Jf La Peyre, Kimberly S. Reece, C Azevedo, A Villalba
VIMS Articles
Continuous in vitro cultures of the clam Tapes decussatus parasite Perkinsus atlanticus were established from infected gill fragments, infected haemolymph and parasite hypnospores isolated from infected gill fragments following incubation in Ray's fluid thioglycollate medium (RFTM). No continuous cultures could be initiated from P. atlanticus zoospores. Cultures initiated from hypnospores yielded the highest percentage of continuous cultures (100%, 6/6), followed by cultures initiated from gill fragments (93%, 43/46) and from haemolymph (30%, 3/10). Failures to establish continuous cultures were due to microbial contamination, The source of parasite influenced the success rate, the time taken to establish cultures and the size …
Molecular Diagnostics, Field Validation, And Phylogenetic Analysis Of Quahog Parasite Unknown (Qpx), A Pathogen Of The Hard Clam Mercenaria Mercenaria, Na Stokes, Lm Ragone Calvo, Kimberly S. Reece, Em Burreson
Molecular Diagnostics, Field Validation, And Phylogenetic Analysis Of Quahog Parasite Unknown (Qpx), A Pathogen Of The Hard Clam Mercenaria Mercenaria, Na Stokes, Lm Ragone Calvo, Kimberly S. Reece, Em Burreson
VIMS Articles
Quahog Parasite Unknown (QPX) is a protistan parasite that causes disease and mortality in the hard clam Mercenaria mercenaria. PCR primers and DNA oligonucleotide probes were designed and evaluated for sensitivity and specificity for the QPX organism specifically and for the phylum Labyrinthulomycota in general. The best performing QPX-specific primer pair amplified a 665 bp region of the QPX small-subunit ribosomal DNA (SSU rDNA) and detected as little as 1 fg cloned QPX SSU rDNA and 20 fg QPX genomic DNA. The primers did not amplify DNA of uninfected hard clams M. mercenaria or of the thraustochytrids Schizochytrium aggregatum, Thraustochytrium …
Relative Strengths Of Competition For Space And Food In A Sessile Filter Feeder, David P. Lohse
Relative Strengths Of Competition For Space And Food In A Sessile Filter Feeder, David P. Lohse
VIMS Articles
Previous workers have demonstrated that sessile filter feeders compete for food and space, but little is known about the relative strengths of these two processes. To determine this, the density and position of barnacles (Balanus improvisus) in a unidirectional current were manipulated to alter the amount of competition for space and food, respectively. Results indicated that competition for space significantly reduced growth, and marginally reduced survivorship. Competition for food was also detected, but only among uncrowded individuals; thus, it appears to be the weaker of the two interactions. However, under crowded conditions, downstream individuals actually grew more than those upstream. …
Nuclear And Mitochondrial Dna Markers For Specific Identification Of Istiophorid And Xiphiid Billfishes, Jan Mcdowell, John Graves
Nuclear And Mitochondrial Dna Markers For Specific Identification Of Istiophorid And Xiphiid Billfishes, Jan Mcdowell, John Graves
VIMS Articles
Independent molecular markers based on mitochondrial and nuclear DNA were developed to provide positive identification of istiophorid and xiphiid billfishes (marlins, spearfishes, sailfish, and swordfish). Both classes of markers were based on amplification of short segments (
Two Epizootic Diseases In Chesapeake Bay Commercial Clams, Mya Arenaria And Tagelus Plebeius, Cf Dungan, Rm Hamilton, Kl Hudson, Cb Mccollough, Kimberly S. Reece
Two Epizootic Diseases In Chesapeake Bay Commercial Clams, Mya Arenaria And Tagelus Plebeius, Cf Dungan, Rm Hamilton, Kl Hudson, Cb Mccollough, Kimberly S. Reece
VIMS Articles
Declining Chesapeake Bay harvests of softshell clams, together with historical and emerging reports of epizootic diseases in Mya arenaria, prompted a survey in summer 2000 of the health status of selected commercial clam populations. All sampled populations (8 M. arenaria softshell clam, 2 Tagelus plebeius razor clam) were infected by Perkinsus sp. protozoans at prevalences ranging from 30 to 100% of sampled clams. Nucleotide sequences for the internal transcribed spacer (ITS) region of the rRNA gene complex were determined for clonal in vitro Perkinsus sp. isolates propagated from both M arenaria and T plebeius. Multiple polymorphic sequences were amplified from …
Study Of Perkinsosis In The Carpet Shell Clam Tapes Decussatus In Galicia (Nw Spain). I. Identification Of The Aetiological Agent And In Vitro Modulation Of Zoosporulation By Temperature And Salinity, Sm Casas, A Villalba, Kimberly S. Reece
Study Of Perkinsosis In The Carpet Shell Clam Tapes Decussatus In Galicia (Nw Spain). I. Identification Of The Aetiological Agent And In Vitro Modulation Of Zoosporulation By Temperature And Salinity, Sm Casas, A Villalba, Kimberly S. Reece
VIMS Articles
Morphological characters of zoosporulation stages and DNA sequence of the internal transcribed spacer (ITS) region and the small subunit ribosomal RNA (SSU rRNA) gene confirmed that the aetiological agent of perkinsosis in the clam Tapes decussatus from Galicia (NW Spain) was Perkinsus atlanticus Azevedo, 1989. In vitro modulation by temperature and salinity of the zoosporulation of the parasite was studied. The optimum temperature range for zoosporulation was 19 to 28degreesC. The temperature range allowing zoosporulation in vitro was 15 to 32degreesC, which is broader than previously reported (24 to 28degreesC) for R atlanticus, and strongly suggests that zoospores can be …
An Evaluation Of Pop-Up Satellite Tags For Estimating Postrelease Survival Of Blue Marlin (Makaira Nigricans) From A Recreational Fishery, John Graves, Brian E. Luckhurst, Eric D. Prince
An Evaluation Of Pop-Up Satellite Tags For Estimating Postrelease Survival Of Blue Marlin (Makaira Nigricans) From A Recreational Fishery, John Graves, Brian E. Luckhurst, Eric D. Prince
VIMS Articles
No abstract provided.
Properties Of The Residuals From Two Tag-Recovery Models, Robert J. Latour, John M. Hoenig, Kenneth H. Pollock
Properties Of The Residuals From Two Tag-Recovery Models, Robert J. Latour, John M. Hoenig, Kenneth H. Pollock
VIMS Articles
No abstract provided.
Horizontal And Vertical Movements Of Juvenile Bluefin Tuna (Thunnus Thynnus), In Relation To Oceanographic Conditions Of The Western North Atlantic, Determined With Ultrasonic Telemetry, Richard Brill, Molly Lutcavage, Greg Metzger, Peter Bushnell, Michael D. Arendt, Jon Lucy, Cheryl Watson, David Foley
Horizontal And Vertical Movements Of Juvenile Bluefin Tuna (Thunnus Thynnus), In Relation To Oceanographic Conditions Of The Western North Atlantic, Determined With Ultrasonic Telemetry, Richard Brill, Molly Lutcavage, Greg Metzger, Peter Bushnell, Michael D. Arendt, Jon Lucy, Cheryl Watson, David Foley
VIMS Articles
We employed ultrasonic transmitters to follow (for up to 48 h) the horizontal and vertical movements of five juvenile (6.8-18.7 kg estimated body mass) bluefin tuna (Thunnus thynnus) in the western North Atlantic (off the eastern shore of Virginia). Our objective was to document the fishes' behavior and distribution in relation to oceanographic conditions and thus begin to address issues that currently limit population assessments based on aerial surveys. Estimation of the trends in adult and juvenile Atlantic bluefin tuna abundance by aerial surveys, and other fishery-independent measures, is considered a priority. Juvenile bluefin tuna spent the majority of their …
Hybridization Between Two Serranids, The Coney (Cephalopholis Fulva) And The Creole-Fish (Paranthias Furcifer), At Bermuda, Meredith A. Bostrom, Bruce B. Collette, Brian E. Luckhurst, Kimberly S. Reece, John Graves
Hybridization Between Two Serranids, The Coney (Cephalopholis Fulva) And The Creole-Fish (Paranthias Furcifer), At Bermuda, Meredith A. Bostrom, Bruce B. Collette, Brian E. Luckhurst, Kimberly S. Reece, John Graves
VIMS Articles
Intergeneric hybridization between the epinepheline serranids Cephalopholis fulva and Paranthias furcifer in waters off Bermuda was investigated by using morphological and molecular characters. Putative hybrids, as well as members of each presumed parent species, were analyzed for 44 morphological characters and screened for genetic variation at 16 nuclear allozyme loci, two nuclear WDNA loci, and three mitochondrial (mt)DNA gene regions. Four of 16 allozyme loci, creatine kinase (CK-B*), fumarase (FH*), isocitrate dehydrogenase (ICDH-S*), and lactate dehydrogenase (LDH-B*), were unique in C. fulva and R furcifer. Restriction fragments of two nuclear DNA intron regions, an actin gene intron and the second …
Age And Growth Of The Smooth Dogfish (Mustelus Canis) In The Northwest Atlantic Ocean, Christina L. Conrath, J Gelsleichter, John A. Musick
Age And Growth Of The Smooth Dogfish (Mustelus Canis) In The Northwest Atlantic Ocean, Christina L. Conrath, J Gelsleichter, John A. Musick
VIMS Articles
The northwest Atlantic population of smooth dogfish (Mustelus canis) ranges from Cape Cod, Massachusetts, to South Carolina. Although M. canis is seasonally abundant in this region, very little is known about important aspects of its biology, such as growth and reproductive rates. In the early 1990s, commercial fishery landings of smooth dogfish dramatically increased on the east coast of the United States. This study investigated growth rates of the east coast M. canis population through analysis of growth patterns in vertebral centra. Marginal increment analysis, estimates of precision, and patterns in seasonal growth supported the use of vertebrae to age …
Spawning Of American Shad (Alosa Sapidissima) And Striped Bass (Morone Saxatilis) In The Mattaponi And Pamunkey Rivers, Virginia, Donna M. Bilkovic, John E. Olney, Carl H. Hershner
Spawning Of American Shad (Alosa Sapidissima) And Striped Bass (Morone Saxatilis) In The Mattaponi And Pamunkey Rivers, Virginia, Donna M. Bilkovic, John E. Olney, Carl H. Hershner
VIMS Articles
No abstract provided.