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- Aquatic Health Sciences Peer-Reviewed Articles (6)
- Fisheries Science Peer-Reviewed Articles (2)
- Bight (1)
- Biological Sciences Peer-Reviewed Articles (1)
- Chesapeake-Bay; Salinity; Susceptibility; Temperature; Epizootiology (1)
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- Crassostrea Gigas; Triploid; Growth; Survival; Disease Susceptibility; Virginia (1)
- ESL Publications (1)
- Life-History Patterns; Flatfish; Biology; Reproduction; Parameters; Population; Fishes; Waters; Sole (1)
- Monoclonal-Antibodies; Atlantic Salmon (1)
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- Renibacterium salmoninarum; bacterial kidney disease (1)
Articles 1 - 10 of 10
Full-Text Articles in Life Sciences
Antigenic And Functional Characterization Of P57 Produced By Renibacterium Salmoninarum, Gd Wiens, Ms Chien, Jr Winton, Sl Kaattari
Antigenic And Functional Characterization Of P57 Produced By Renibacterium Salmoninarum, Gd Wiens, Ms Chien, Jr Winton, Sl Kaattari
VIMS Articles
Renibacterium salmoninarum, the causative agent of bacterial kidney disease, produces large quantities of a 57-58 kDa protein (p57) during growth in broth culture and during infection of salmonid fish. Biological activities of secreted p57 include agglutination of salmonid leucocytes and rabbit erythrocytes. We define the location of epitopes on p57 recognized by agglutination-blocking monoclonal antibodies (MAbs) 4C11, 4H8 and 4D3, and demonstrate that the majority of secreted p57 is a monomer that retains salmonid leucocyte agglutinating activity. The 3 MAbs bound a recombinant, amino-terminal fragment of p57 (211 aa) but not a carboxy-terminal fragment (315 aa) demonstrating that the neutralizing …
Elevated Temperature Treatment As A Novel Method For Decreasing P57 On The Cell Surface Of Renibacterium Salmoninarum, Jon D. Piganelli, Gregory D. Weins, Stephen L. Kaattari
Elevated Temperature Treatment As A Novel Method For Decreasing P57 On The Cell Surface Of Renibacterium Salmoninarum, Jon D. Piganelli, Gregory D. Weins, Stephen L. Kaattari
VIMS Articles
Renibacterium salmoninarum is a Gram-positive diplo-bacillus and the causative agent of bacterial kidney disease, a prevalent disease of salmonid fish. Virulent isolates of R. salmoninarum have a hydrophobic cell surface and express the 57-58 kDa protein (p57). Here we have investigated parameters which effect cell hydrophobicity and p57 degradation. Incubation of R, salmoninarum cells at 37 degrees C for >4 h decreased cell surface hydrophobicity as measured by the salt aggregation assay, and decreased the amount of cell associated p57. Incubation of cells at lower temperatures (22, 17, 4 or -20 degrees C) for up to 16 h did not …
Environmental Factors And The Infectious Disease Caused By The Protozoan Parasite, Perkinsus Marinus, In Eastern Oysters (Crassostrea Virginica), Fle Chu
VIMS Articles
Temperature and salinity are two important factors limiting the distribution and abundance of Perkinsus marinus, a protozoan parasite of Eastern oysters (Crassostrea virginica). Results of laboratory studies ale consistent with field observations and clearly demonstrate that P. marinus susceptibility and disease advancement are positively correlated with temperature, salinity and in situ number of infective cells. Laboratory findings also suggest that environmental degradation may enhance the epizootic, although disease caused by P. marinus in oysters is known to be predominantly exacerbated by elevated temperature and salinity. Oysters cellular defence mechanisms appear ineffective in defence against P. marinus. Also, pollutant esposure caused …
Morphometric Separation Of Annual Cohorts Within Mid-Atlantic Bluefish, Pomatomus Saltatrix, Using Discriminant Function Analysis, Herbert Austin, Daniel Scoles, Allison J. Abell
Morphometric Separation Of Annual Cohorts Within Mid-Atlantic Bluefish, Pomatomus Saltatrix, Using Discriminant Function Analysis, Herbert Austin, Daniel Scoles, Allison J. Abell
VIMS Articles
Larval and tagging studies neither support nor refute the existence of two mid-Atlantic stocks of bluefish, Pomatomus saltatrix, one that spawns just south of Hatteras, North Carolina, in the spring, and the other off New England in the summer. Subsequent studies have suggested either a continuous spawning with two survival events, or two distinct spawning events, one in spring south of Hatteras, NC, and a second in mid-summer from Long Island to New England. Genetic studies have supported a single stock because they show the existence of a single gene pool. A stepwise linear discriminant function analysis (DFA) of morphometrics …
Potential Role Of Protease-Antiprotease Interactions In Perkinsus Marinus Infection In Crassostrea Spp., M Faisal, Jl Oliver, Sl Kaataari
Potential Role Of Protease-Antiprotease Interactions In Perkinsus Marinus Infection In Crassostrea Spp., M Faisal, Jl Oliver, Sl Kaataari
VIMS Articles
Perkinsus marinus causes devastating losses in populations of the eastern oyster (Crassostrea virginica). Our studies have demonstrated that P. marinus secretes extracellular serine proteases which enhance parasite propagation and compromise host defences. Crassostrea virginica. however, possesses several inhibitors of these proteases. The Pacific oyster (C. gigas) is resistant to P. marinus and possesses protease inhibitors with significantly higher specific activities than those in C. virginica. Interestingly, Crassostrea spp. themselves, elaborate metalloprotease activities which can be detected in their plasma, and are increased during P. marinus infections. Together our work suggests that there may be a broad spectrum of humoral host …
Teleost Antibody Structure: Simple Prototype Or Elegant Alternative?, Sl Kaattari, Jv Klemer, Da Evans
Teleost Antibody Structure: Simple Prototype Or Elegant Alternative?, Sl Kaattari, Jv Klemer, Da Evans
VIMS Articles
Teleosts possess mechanism(s) by which they can generate considerable structural diversity within their tetrameric antibody molecules. In salmonids, this diversity is generated through a process of random polymerisation of the constituent monomeric subunits rather than dependency upon isotypic gene diversity. Thus, one gene product can give rise to as many as six different structural forms of immunoglobulin. In contrast to mammals, evidence suggests that this polymerisation process occurs late in the secretory process and not within the endoplasmic reticulum. This assembly process is likely to be important in the generation of teleost antibody functional diversity, thereby potentially simulating isotypy.
Isolation Of Aphanomyces Sp(P). Associated With Skin Lesions And Mortalities In The Striped (Mugil Cephalus) And The Thin Lip (Liza Ramada) Grey Mullets, Aa Shaheen, E Elsayed, M Faisal
Isolation Of Aphanomyces Sp(P). Associated With Skin Lesions And Mortalities In The Striped (Mugil Cephalus) And The Thin Lip (Liza Ramada) Grey Mullets, Aa Shaheen, E Elsayed, M Faisal
VIMS Articles
Aphanomyces spp. were isolated from skin erosions and ulcers of cultured Striped and thin lip grey mullets. Fungi with non-septated hyphae were found in wet mount preparations of skin. Samples inoculated on glucose peptone liquid medium for three successive steps then mycelial growths were transferred into glucose peptone yeast extract agar medium. Aphanomyces spp. were successfully isolated, in axenic cultures, from seven out of 35 fish samples. The cultures were maintained on glucose peptone yeast extract liquid media. Formation of sporangia in tap water that contained hemp seeds confirmed that the grey mullet isolates are members of tlx genus Aphanomyces. …
Use Of Molecular Tools For Mollusc Disease Diagnosis, Fcj Berthe, Em Burreson, M Hine
Use Of Molecular Tools For Mollusc Disease Diagnosis, Fcj Berthe, Em Burreson, M Hine
VIMS Articles
Recently, an increasing number of research teams arc engaged in developing DNA-based diagnostic techniques for mollusc pathogens. These techniques are slowly moving from development in specialised laboratories for research purposes, to routine application and are expected to find an increasing use in routine disease monitoring programs in mollusc aquaculture and in efforts to prevent the spread of pathogens within and between nations. Considering the probable development and interest in these diagnostic techniques, the potential for wide applications in aquaculture and the inherent problems currently associated with their use, it appears necessary to address these issues.
Comparative Field Study Of Crassostrea Gigas (Thunberg, 1793) And Crassostrea Virginica (Gmelin, 1791) In Relation To Salinity In Virginia, Gustavo W. Calvo, Mark Luckenbach, Standish K. Allen, Eugene Burreson
Comparative Field Study Of Crassostrea Gigas (Thunberg, 1793) And Crassostrea Virginica (Gmelin, 1791) In Relation To Salinity In Virginia, Gustavo W. Calvo, Mark Luckenbach, Standish K. Allen, Eugene Burreson
VIMS Articles
To evaluate and compare the performance of triploid juvenile C. gigas (mean shell height = 19.2 mm) and triploid juvenile Crassostrea virginica (mean shell height = 31.7 mm), 600 oysters of each species were deployed for 1 year in floating mesh cages at three replicate sites within low, medium, and high salinity regimes (respectively,25%) in the Chesapeake Bay and the Atlantic Coast of Virginia. The comparative performance of the two oyster species varied with salinity. At low salinity sites, cumulative mortality of C. virginica (10%) was significantly (P < .05) lower than that of C. gigas (63%), and over-all mean growth rate of C. virginica (2.9 mm mo(-1)) was significantly (P < .05) higher than that of C. gigas (1.6 mm mo(-1)). At medium salinity sites, survival and growth rate of C. virginica and C. gigas were nor significantly (P > .05) different. Both species experienced moderately high cumulative mortality at the medium …
Age, Growth, Longevity, And Mortality Of Blackcheek Tonguefish, Symphurus Plagiusa (Cynoglossidae : Pleuronectiformes), In Chesapeake Bay, Virginia, Mark R. Terwilliger, Thomas A. Munroe
Age, Growth, Longevity, And Mortality Of Blackcheek Tonguefish, Symphurus Plagiusa (Cynoglossidae : Pleuronectiformes), In Chesapeake Bay, Virginia, Mark R. Terwilliger, Thomas A. Munroe
VIMS Articles
Age structure, longevity, and mortality were determined for a population of blackcheek tonguefish, Symphurus plagiusa, in Chesapeake Bay. Blackcheek tonguefish (36-202 mm TL) were randomly collected by means of otter trawl in lower Chesapeake Bay and major Virginia tributaries (James, York, and Rappahannock rivers) from April 1994 through August 1995. Ages were determined by interpreting growth increments on thin transverse sections of sagittal otoliths. Marginal increment analysis showed that a single annulus was formed in June of each year. Blackcheek tonguefish caught during this study reached a maximum age of 5+ years. Growth was rapid during the first year, then …