Life Sciences Commons^™

Development And Application Of A Reverse Genetics System For Zika Virus, Jordan C. Frank

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Zika virus (ZIKV) has emerged in many regions of the world, with infection outcomes spanning from no apparent illness to crippling nervous system disease. ZIKV and its close relatives, West Nile virus, Japanese encephalitis virus, dengue virus, and yellow fever virus are primarily transmitted by mosquitoes. Three ZIKVs were selected: MR-766 (Uganda, 1947), P6-740 (Malaysia, 1966), and PRVABC-59 (Puerto Rico, 2015), whose place of origin and time of isolation differ substantially. Stable, complementary DNA (cDNA) copies of the three ZIKV RNA genomes were cloned to examine the significance of viral and host genetic variations in directing ZIKV infection outcomes. Using …

Efficacy Of Translocation As A Management Tool For Urban Mule Deer In Utah, Channing R. Howard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An increase in urbanization in the United States has led to an increase in human-wildlife interactions with deer (Odocoileus spp.) which have been able to adapt and thrive in these urban environments. In Utah, urbanization has occurred along the Wasatch Front which was once traditional mule deer (Odocoileus hemionus) winter range habitat. This urban expansion coupled with an increasing use of these urban areas by mule deer, have led to increasing conflicts with deer. Overabundant urban deer have led to increased concerns over safety from deer-vehicle collisions, and damage to personal property including gardens and landscaping. …

Influence Of Environmental Variables On Survival Rates Of Pronghorn (Antilocapra Americana) Neonates Across Idaho, Brett R. Panting

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was completed to better understand pronghorn antelope (Antilocapra americana) populations found throughout Idaho. Antelope were studied in three separate and distinct study areas. The Big Desert, Camas Prairie, and Little Lost and Pahsimeroi valleys were all selected as study sites. Idaho Department of Fish and Game (IDFG) is concerned with current pronghorn populations found throughout Idaho. Pronghorn are a valued big game species found in Idaho. Increasing pronghorn populations in Idaho is a focus of IDFG.

We captured and VHF-collared pronghorn fawns found in our three study areas. Fawns were monitored daily with telemetry equipment for …

Identification Of Sperm Chromatin Proteins As Candidate Markers Of Stallion Fertility, Chelsea C. Ketchum

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

During spermatogenesis, histones are largely replaced by transition proteins and protamines in normal stallions. Incomplete nucleoprotein exchange results in the abnormal retention of histones and transition proteins, which is an indicator of poor sperm quality. Equine nucleoprotein exchange has not previously been investigated in detail, so that equine sperm chromatin quality problems, which are often responsible for poor breeding performance of stallions, are not well understood. In order to characterize chromatin remodeling events in stallion spermatogenesis and to identify proteins indicative of sperm chromatin defects, such as excessive amounts of histones, we identified antibodies that recognize equine testis-specific proteins of …

Impact Of Basal Diet On Obesity Phenotype Of Recipient Mice Following Fecal Microbiome Transfer From Obese Or Lean Human Donors, Daphne Michelle Rodriguez Jimenez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The composition of the gut microbiome can be affected by environmental factors, such as diet. The Western dietary pattern is associated with microbiome dysbiosis and adverse health outcomes, including obesity and metabolic disorders. The objective of this study was to examine the effect of gut microbiota from lean or obese human donors on metabolism and weight gain in recipient mice fed one of three basal diets: 1) the standard AIN93G diet, which promotes rodent health; 2) the total Western diet (TWD), which mimics the American dietary pattern and promotes inflammation-associated colorectal carcinogenesis; and 3) a 45% high fat diet-induced obesity …

Phenotypic Association Between Lactose And Other Milk Components In Western Us Dairy Herds And Japan, Takuji Asami

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Lactose in milk has relatively low variation regardless of season, breed, or country. The study of lactose concentration and correlation among other milk components is limited. Furthermore, dairy farmers have limited access to the lactose data and are not familiar with it. This study was conducted to: 1) investigate the phenotypic correlation between lactose and other milk components; and 2) determine the importance of lactose for dairy herds.

Monthly DHIA records from Utah (DHIA), Dairy Herd Performance Test (DHTP) records from Ibaraki, Japan, and California herd average data (CHAD) covering 27 states were used to analyze the relationships between milk …

Assessment Of Microchimerism Following Somatic Cell Nuclear Transfer And Natural Pregnancies In Goats (Capra Aegagrus Hircus), Kirsten Karen Gash

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Somatic cell nuclear transfer (SCNT) is a powerful tool for production of transgenic animals for various biomedical and agricultural applications. For instance, our group is using SCNT to produce transgenic goats to study the role of cardiac fibrosis in initiation and progression of atrial fibrillation. There is a possibility of cell transfer from a transgenic fetus to its non-transgenic surrogate mother, known as fetal microchimerism; from a transgenic mother to non-transgenic fetus, maternal microchimerism and from a transgenic twin to non-transgenic twin in utero. Initially, we have assessed the presence of fetal microchimerism in tissue samples from non-transgenic surrogates …

Effect Of Tannin-Containing Legume Hays On Enteric Methane Emissions And Nitrogen Partitioning In Beef Cattle, Elizabeth K. Stewart

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cattle are responsible for greenhouse gas emissions such as carbon dioxide, methane and nitrous oxide. In particular, the cow-calf phase of production accounts for approximately 80 percent of the total beef production system greenhouse gas emissions. Tannins are chemical compounds found in certain forages and they have the potential to help reduce these negative environmental impacts. Thus, given that the cow-calf phase often relies on feeding hay, feeding tannin-containing hays may represent a significant mitigation practice.

With my MS program, I sought to explore whether tannin-containing hays fed to mother cows and heifers influence methane and nitrogen emissions relative to …

U.S. Consumers’ Perception, Intention, And Purchase Behavior Of Grass-Fed Beef, Elizabeth K. Crandall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this research study was to compile regional profiles of the consumers who intend to consume grass-fed beef in the U.S. and to create marketing strategies that would assist producers in marketing their product to consumers. The researcher sent an online survey to a panel of 484 consumers across the U.S. to learn about their perceptions of and intention to purchase grass-fed beef.

Respondents had a weak, positive attitude toward purchasing grass-fed beef but had low knowledge of production practices. These consumers had a desire to eat healthier; however, they wanted meat that was priced right and had …

Development And Characterization Of Reporter-Expressing Zika Viruses, Michael E. Woolley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In recent years, Zika virus (ZIKV) has garnered worldwide attention due to its epidemic spread throughout the Americas and due to the newly recognized link between ZIKV infection and neurological diseases, including microcephaly and Guillain-Barré syndrome. ZIKV is a mosquito-borne member of the genus Flavivirus, which includes the other prominent human pathogens Japanese encephalitis virus, West Nile virus, dengue virus, and yellow fever virus. Many questions about the biology of ZIKV and how it causes disease remain unanswered. Furthermore, there currently are no vaccines or licensed antiviral drugs available to treat ZIKV infection. The goal of this study was to …

Evaluation Of Therapeutics For An Enterovirus 71 Infection In An Ag129 Mouse Model, Christopher Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Discovered in 1969 in California, enterovirus 71 (EV-71) is a serious cause of disease in young children. It is one of the major causative agents of hand, food, and mouth disease (HFMD), and can produce neurological complications, such as meningitis, encephalitis, and an acute flaccid paralysis. For serious cases, the fatality rate can be up to 26%, almost exclusively in young children.

While the virus was initially discovered in the United States, it was soon detected in the Eastern hemisphere, causing outbreaks in Europe and Asia. The largest outbreak occurred in Taiwan in 2008, with approximately 490,000 cases and 128 …

Evaluation Of Oocyte Developmental Competence And Potential Strategies To Improve Oocyte Quality, Min Yang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Assisted reproductive technologies (ARTs) have now been extensively used to promote reproductive efficiency as a fertility treatment not only in human medicine but also animal reproduction. ARTs serve as an important tool to advance the fundamental knowledge of reproductive processes. The quality of female’s eggs defines its ability to undergo maturation, fertilization, and development. This quality is determined by various factors and is crucial for the success of ARTs. Any alternations happening during the egg growth and maturation process can result in the decreased quality, which could have long-lasting effects on development. Improving the developmental efficiency of the egg is …

Targeted-Grazing As A Fuels Reduction Treatment: Evaluation Of Vegetation Dynamics And Utilization Levels, Travis Decker

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wildfires have caused concern as they have increased in severity and intensity over the last few decades. Land managers have sought management actions to mitigate the risk of wildfire by reducing fuel loads, thus decreasing wildfire intensity. Camp Williams is a National Guard camp near Bluffdale, Utah, where small arms and artillery training occurs. Managers at Camp Williams have created fuel breaks by implementing targeted sheep and goat grazing to remove fine fuel and thin brush. Management objectives set utilization of fine fuels (herbaceous) at 80% by weight. Questions arose regarding the ecological impact of the prescribed grazing rates in …

Bovine Mastitis Resistance: Novel Quantitative Trait Loci And The Role Of Bovine Mammary Epithelial Cells, Jacqueline P. Kurz

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bovine mastitis, or inflammation of the mammary gland, has substantial economic and animal welfare implications. A genetic basis for mastitis resistance traits is recognized and can be used to guide selective breeding programs. The discovery of regions of the genome associated with mastitis resistance, and knowledge of the underlying molecular mechanisms responsible, can facilitate development of efficient mastitis control and therapeutic strategies. The objectives of this dissertation research were to identify sites of genetic variation associated with mastitis resistance, and to define the contributions of the milk-secreting epithelial cells to mammary gland immune responses and mastitis resistance. Twenty seven regions …