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Animal Sciences

TÜBİTAK

2019

Flow cytometry

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Effect Of Various Antioxidants And Their Combinations On Bull Semen Cryopreservation, Şükrü Güngör, Ayhan Ata, Muhammed Enes İnanç, John P. Kastelic Jan 2019

Effect Of Various Antioxidants And Their Combinations On Bull Semen Cryopreservation, Şükrü Güngör, Ayhan Ata, Muhammed Enes İnanç, John P. Kastelic

Turkish Journal of Veterinary & Animal Sciences

Our objective was to evaluate the effects of adding 50 mM trehalose (TRE), 1 mM cysteine (CYS), or 400 µg catalase (CAT), individually or in combinations, to a Tris-based semen extender and determine the effects on fresh, chilled-equilibrated, and frozen-thawed bull sperm. Ejaculates from three Holstein bulls (1-3 years old) were pooled, divided into six equal portions, and put into a Tris-based extender supplemented with no additive (control), TRE, CYS, CAT, TRE + CYS, or TRE + CYS + CAT. In chilled-equilibrated semen, motility was highest in CAT but lowest in the control (P < 0.05). In thawed semen, motility was lower (P < 0.05) in CAT than in all other groups, which were not different. Based on a hypoosmotic swelling test (HOS test), TRE, CYS, and their combinations had higher (P < 0.05) percentages of sperm with an intact plasma membrane than the control or CAT groups. The TRE group had the least (P < 0.05) morphologically abnormal sperm. Plasma membrane and acrosomal integrity (PMAI) and high mitochondrial membrane potential (HMMP) values were highest in TRE and TRE + CYS and were lower (P < 0.05) in the control. Furthermore, HMMP was positively correlated with PMAI and HOS tests, but negatively correlated with major + minor abnormalities (r = 0.66, 0.242, and -0.349). In conclusion, the addition of TRE, with or without CYS, to a Tris-based semen extender improved several aspects of the frozen-thawed quality of bull semen. However, addition of CAT, by itself or in combination with other additives, did not protect bull sperm against cryopreservation-induced defects.