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Full-Text Articles in Life Sciences
Genome Size Variation Among Natural Populations Of Brachypodium Distachyon And B. Hybridum Collected From Different Regions Of Turkey, Gülsemi̇n Savaş Tuna, İsmet Başer, Meti̇n Tuna
Genome Size Variation Among Natural Populations Of Brachypodium Distachyon And B. Hybridum Collected From Different Regions Of Turkey, Gülsemi̇n Savaş Tuna, İsmet Başer, Meti̇n Tuna
Turkish Journal of Botany
Brachypodium distachyon (L.) P.Beauv. is a model grass species that is useful for studying temperate cereal, forage, and energy crops. In this study we aimed to determine the genome size and species identity in the collections of the B. distachyon complex that included samples from 56 different locations in Turkey, applying chromosome counting and nuclear genome size evaluation by flow cytometry method (FCM). Moreover, the study examined whether diverse geographical conditions at the collecting sites influence the genome size. Chromosomal analysis revealed that the number of B. distachyon (x = 5, 2n = 10) genotypes was 48 and the number …
Mesophyll Protoplast Isolation Technique And Flow Cytometry Analysis Of Ancient Platycladus Orientalis (Cupressaceae), Qianyi Zhou, Zhaohong Jiang, Yiming Li, Tian Zhang, Hailan Zhu, Fei Zhao, Zhong Zhao
Mesophyll Protoplast Isolation Technique And Flow Cytometry Analysis Of Ancient Platycladus Orientalis (Cupressaceae), Qianyi Zhou, Zhaohong Jiang, Yiming Li, Tian Zhang, Hailan Zhu, Fei Zhao, Zhong Zhao
Turkish Journal of Agriculture and Forestry
Platycladus orientalis (Cupressaceae) is a native tree species used widely for landscaping and afforestation in arid and semiarid areas in Asia. Ancient P. orientalis trees not only have an important humanistic and historical value but also have a significant scientific value in aging mechanism research. Mesophyll protoplasts are an important material for studies of plant regeneration, transient gene expression, and senescence. Although an easy and effective technique of mesophyll protoplast isolation from mature trees is urgently needed, the isolation parameters have great material specificity. The young tissue of plants is an ideal material for protoplast preparation. In this study, we …
Effect Of Various Antioxidants And Their Combinations On Bull Semen Cryopreservation, Şükrü Güngör, Ayhan Ata, Muhammed Enes İnanç, John P. Kastelic
Effect Of Various Antioxidants And Their Combinations On Bull Semen Cryopreservation, Şükrü Güngör, Ayhan Ata, Muhammed Enes İnanç, John P. Kastelic
Turkish Journal of Veterinary & Animal Sciences
Our objective was to evaluate the effects of adding 50 mM trehalose (TRE), 1 mM cysteine (CYS), or 400 µg catalase (CAT), individually or in combinations, to a Tris-based semen extender and determine the effects on fresh, chilled-equilibrated, and frozen-thawed bull sperm. Ejaculates from three Holstein bulls (1-3 years old) were pooled, divided into six equal portions, and put into a Tris-based extender supplemented with no additive (control), TRE, CYS, CAT, TRE + CYS, or TRE + CYS + CAT. In chilled-equilibrated semen, motility was highest in CAT but lowest in the control (P < 0.05). In thawed semen, motility was lower (P < 0.05) in CAT than in all other groups, which were not different. Based on a hypoosmotic swelling test (HOS test), TRE, CYS, and their combinations had higher (P < 0.05) percentages of sperm with an intact plasma membrane than the control or CAT groups. The TRE group had the least (P < 0.05) morphologically abnormal sperm. Plasma membrane and acrosomal integrity (PMAI) and high mitochondrial membrane potential (HMMP) values were highest in TRE and TRE + CYS and were lower (P < 0.05) in the control. Furthermore, HMMP was positively correlated with PMAI and HOS tests, but negatively correlated with major + minor abnormalities (r = 0.66, 0.242, and -0.349). In conclusion, the addition of TRE, with or without CYS, to a Tris-based semen extender improved several aspects of the frozen-thawed quality of bull semen. However, addition of CAT, by itself or in combination with other additives, did not protect bull sperm against cryopreservation-induced defects.