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The Roles Of Cyp19ala And Dmrt1 During Gonadal Sex Differentiation And Sex Change In Orange-Spotted Grouper, Epinephelus Coioides, Chia-Yung Chen, Ya-Ju Tsai, Ching-Fong Chang
The Roles Of Cyp19ala And Dmrt1 During Gonadal Sex Differentiation And Sex Change In Orange-Spotted Grouper, Epinephelus Coioides, Chia-Yung Chen, Ya-Ju Tsai, Ching-Fong Chang
Journal of Marine Science and Technology
Sex steroids play a crucial role in gonadal sex differentiation in many nonmammalian vertebrates, including hermaphroditic teleosts. The current study investigates the mRNA expression levels of gonadal cyp19a1a, dmrt1, sf-1, dax-1 and 11β-hsd in the protogynous orange-spotted grouper, Epinephelus coioides, at certain stages during sex differentiation and sex change. Among the genes whose mRNA levels were determined through quantitative RT-PCR analysis, cyp19a1a was predominantly expressed in the ovary, and its expression was much lower in the testis. The level of cyp19a1a expressed at 150 dah was significantly high enough to trigger the pre-gyno female development of protogynous (subadult) orange-spotted grouper. …