Life Sciences Commons^™

Does Baylisascaris Procyonis Impact Raccoon (Procyon Lotor) Genetics?, Matthew E. Ingle, Stephen G. Dunbar, Jaynee L. Bartsch, Kyle J. Culbertson, Taylor A. Fulton, Katherine R. Guffey, Aubrey J. Juris, Ashlie N. Nolan, Daniel P. Nordquist, Carrie E. Rowlands, Joshua A. Sitler

Stephen Dunbar, PhD

Raccoons (Procyon lotor) are the final host for raccoon roundworms (Baylisascaris procyonis). Raccoon roundworm is the leading cause of a dangerous neurological disease, known as larva migrans encephalopathy. Phylogenetic trees illustrate co-evolutionary events between species living in a symbiotic relationship with each other. Throughout the coevolution of host and parasite, many aspects of a population affect the way the members interact with one another and with symbiotic species. In order to evaluate the relationship between host and parasite in regards to diet, we isolated DNA from intestinal wall tissue, amplified a portion of exon 2 from MHC II, and sent …

Goats Display Audience-Dependent Human-Directed Gazing Behaviour In A Problem-Solving Task, Christian Nawroth, Jemma M. Brett, Alan G. Mcelligott

Christian Nawroth, PhD

Domestication is an important factor driving changes in animal cognition and behaviour. In particular, the capacity of dogs to communicate in a referential and intentional way with humans is considered a key outcome of how domestication as a companion animal shaped the canid brain. However, the lack of comparison with other domestic animals makes general conclusions about how domestication has affected these important cognitive features difficult. We investigated human-directed behaviour in an ‘unsolvable problem’ task in a domestic, but non-companion species: goats. During the test, goats experienced a forward facing or an away facing person. They gazed towards the forward …

Human Head Orientation And Eye Visibility As Indicators Of Attention For Goats (Capra Hircus), Christian Nawroth, Alan G. Mcelligott

Christian Nawroth, PhD

Animals domesticated for working closely with humans (e.g. dogs) have been shown to be remarkable in adjusting their behaviour to human attentional stance. However, there is little evidence for this form of information perception in species domesticated for production rather than companionship. We tested domestic ungulates (goats) for their ability to differentiate attentional states of humans. In the first experiment, we investigated the effect of body and head orientation of one human experimenter on approach behaviour by goats. Test subjects (N = 24) significantly changed their behaviour when the experimenter turned its back to the subjects, but did not take …

Are Juvenile Domestic Pigs (Sus Scrofa Domestica) Sensitive To The Attentive States Of Humans? The Impact Of Impulsivity On Choice Behaviour, Christian Nawroth, Mirjam Ebersbach, Eberhard Von Borell

Christian Nawroth, PhD

Previous studies have shown that apes, dogs and horses seem to be able to attribute attentive states to humans. Subjects chose successfully between two persons: one who was able to see the animal and one who was not. Using a similar paradigm, we tested a species that does not rely strongly on visual cues, the domestic pig (Sus scrofa domestica). Subjects could choose between two unfamiliar persons, with only one showing attention, in three different conditions (body, head away, body turned - head front). Subjects (n = 16) only showed a tendency towards …

“Goats That Stare At Men” – Dwarf Goats Alter Their Behaviour In Response To Human Head Orientation But Do Not Spontaneously Use Head Direction As A Cue In A Food-Related Context, Christian Nawroth, Eberhard Von Borell, Jan Langbein

Christian Nawroth, PhD

Recently, comparative research on the mechanisms and species-specific adaptive values of attributing attentive states and using communicative cues has gained increased interest, particularly in non-human primates, birds, and dogs. Here, we investigate these phenomena in a farm animal species, the dwarf goat (Capra aegagrus hircus). In the first experiment, we investigated the effects of different human head and body orientations, as well as human experimenter presence/absence, of a human on the behaviour of goats in a food-anticipating paradigm. Over a 30-sec interval, the experimenter engaged in one of four different postures or behaviours (head and body towards the subject, head …

Judgement Bias In Goats (Capra Hircus): Investigating The Effects Of Human Grooming, Luigi Baciadonna, Christian Nawroth, Alan G. Mcelligott

Christian Nawroth, PhD

Animal emotional states can be investigated by evaluating their impact on cognitive processes. In this study, we used a judgement bias paradigm to determine if shortterm positive human-animal interaction (grooming) induced a positive affective state in goats. We tested two groups of goats and trained them to discriminate between a rewarded and a non-rewarded location over nine training days. During training, the experimental group (nD9) was gently groomed by brushing their heads and backs for five min over 11 days (nine training days, plus two testing days, total time 55 min). During training, the control group (nD10) did not experience …

Juvenile Domestic Pigs (Sus Scrofa Domestica) Use Human-Given Cues In An Object Choice Task, Christian Nawroth, Mirjam Ebersbach, Eberhard Von Borell

Christian Nawroth, PhD

Research on the comprehension of human-given cues by domesticated as well as non-domesticated species has received considerable attention over the last decade. While several species seem to be capable of utilizing these cues, former work with domestic pigs (Sus scrofa domestica) has shown inconclusive results. In this study, we investigated the use of human-given cues in an object choice task by young domestic pigs (N = 17; seven weeks of age) who had very limited human contact prior to the experiments. Subjects had to choose between two bowls of which only one was baited with a reward. Over the course …

Exclusion Performance In Dwarf Goats (Capra Aegagrus Hircus) And Sheep (Ovis Orientalis Aries), Christian Nawroth, Eberhard Von Borell, Jan Langbein

Christian Nawroth, PhD

Using a comparative approach, we investigated the ability of dwarf goats and sheep to use direct and indirect information about the location of a food reward in an object-choice task. Subjects had to choose between two cups with only one covering a reward. Before making a choice, subjects received information about the baited (direct information) or nonbaited cup (indirect information). Both goats and sheep were able to use direct information (presence of food) in the object choice task. After controlling for local enhancement, we found that goats rather than sheep were able to use indirect information (i.e., the absence of …

Object Permanence In The Dwarf Goat (Capra Aegagrus Hircus): Perseveration Errors And The Tracking Of Complex Movements Of Hidden Objects, Christian Nawroth, Eberhard Von Borell, Jan Langbein

Christian Nawroth, PhD

Object permanence is the notion that objects continue to exist even when they are out of an observer’s sight. In mammals, the highest stage of object permanence (Stage 6) has been observed only in primates, whereas other species have shown difficulty in following once-hidden objects, particularly when complex movements are involved. We conducted four experiments to examine the ability of dwarf goats to mentally represent once-hidden objects. In Experiment 1, we investigated whether dwarf goats are prone to commit the A-not-B or perseveration error which is commonly used as a measure for self-control. Subjects here continue to search for an …

Goats Learn Socially From Humans In A Spatial Problem-Solving Task, Christian Nawroth, Luigi Baciadonna, Alan G. Mcelligott

Christian Nawroth, PhD

Domestication drives changes in animal cognition and behaviour. In particular, the capacity of dogs to socially learn from humans is considered a key outcome of how domestication shaped the canid brain. However, systematic evidence for social learning from humans in other domestic species is lacking and makes general conclusions about how domestication has affected cognitive abilities difficult. We assessed spatial and social problem-solving abilities in goats (Capra hircus) using a detour task, in which food was placed behind an inward or outward V-shaped hurdle. Goats performed better in the outward than in the inward detour without human demonstration. Importantly, a …

Are Domestic Pigs (Sus Scrofa Domestica) Able To Use Complex Human-Given Cues To Find A Hidden Reward?, Christian Nawroth, Mirjam Ebersbach, Eberhard Von Borell

Christian Nawroth, PhD

Understanding human-animal interactions in livestock production systems is crucial for improving animal welfare. It is therefore of general interest to investigate how livestock animals obtain information from humans. By using an object-choice paradigm, we investigated whether domestic pigs (n = 4) are able to use a variety of human-given cues, such as different pointing gestures, to find a hidden food reward. In Experiment 1, an experimenter pointed towards a baited location in front of the pig while the extent of the protrusion of his hand from the upper body was varied. Pigs had problems using pointing gestures that did not …

Genetic Demography At The Leading Edge Of The Distribution Of A Rabies Virus Vector, Antoinette J. Piaggio, Amy L. Russell, Ignacio A. Osorio, Alejandro J. Ramirez, Justin W. Fischer, Jennifer L. Neuwald, Annie E. Tibbels, Luis Lecuona, Gary F. Mccracken

Amy L. Russell

Hypogenic Versus Epigenic Subterranean Ecosystem: Lessons From Eastern Iberian Peninsula, Alberto Sendra, Policarp Garay, Vicente M. Ortuño, José D. Gilgado, Santiago Teruel, Ana Sofia P.S. Reboleira

Ana Sofia P.S. Reboleira

Over the last 40 years, hypogenic karst/caves have become well known and hypogene speleogenesis has been used to explain the formation of some of the largest subterranean maze caves. These hypogenic systems involve confined aquifers with upwards flow, responsible for their karstification. Such spaces begun and could be remain isolated from the surface and the contiguous subterranean habitats, including the shallow ones. We studied the invertebrate fauna and the geology/speleogenesis of two recently opened caves from Eastern Iberian Peninsula, which clearly showed features of hypogenic origin. Their scarce faunistic composition, formed by non cave-adapted fauna, is similar to other hypogenic …

Visual Attention And Its Relation To Knowledge States In Chimpanzees, Pan Troglodytes, Megan J. Bulloch, Sarah T. Boysen, Ellen E. Furlong

Ellen Furlong

Primates rely on visual attention to gather knowledge about their environment. The ability to recognize such knowledge-acquisition activity in another may demonstrate one aspect of Theory of Mind. Using a series of experiments in which chimpanzees were presented with a choice between an experimenter whose visual attention was available and another whose vision was occluded, we asked whether chimpanzees understood the relationship between visual attention and knowledge states. The animals showed sophisticated understanding of attention from the first presentation of each task. Under more complex experimental conditions, the subjects had more difficulty with species-typical processing of attentional cues and those …

African Penguins Follow The Gaze Direction Of Conspecifics, Christian Nawroth, Livio Favaro

Christian Nawroth, Ph.D.

Winter Grazing Management, Stephen K. Barnhart, James R. Russell, Douglas L. Karlen, Michael J. Tidman

Douglas L Karlen

Why winter grazing? Beef cow herd and sheep flock records show that winter feeding costs are livestock producers' single largest production expense. Managing through winter weather while keeping feeding costs low is an essential part of maintaining a profitable operation. Iowa's climate generally allows forage growth only during a 7-to-8 month period. Extending the grazing of this forage--even an extra 3 or 4 weeks in late autumn and winter--is an economical way to maintain or increase livestock profitability. Some producers extend the grazing season by using stockpiled forage, whereas others use crop residue, and many combine the use of stockpiled …

Investigation Of The Impact Of Increased Dietary Insoluble Fiber Through The Feeding Of Distillers Dried Grains With Solubles (Ddgs) On The Incidence And Severity Of Brachyspira-Associated Colitis In Pigs, Bailey Lauren Wilberts, Paulo Elias Arruda, Joann M. Kinyon, Timothy S. Frana, Chong Wang, Drew Robert Magstadt, Darin M. Madson, John F. Patience, Eric Ryan Burrough

Bailey (Wilberts) Arruda

Diet has been implicated as a major factor impacting clinical disease expression of swine dysentery and Brachyspira hyodysenteriae colonization. However, the impact of diet on novel pathogenic strongly beta-hemolytic Brachyspira spp. including “B. hampsonii” has yet to be investigated. In recent years, distillers dried grains with solubles (DDGS), a source of insoluble dietary fiber, has been increasingly included in diets of swine. A randomized complete block experiment was used to examine the effect of increased dietary fiber through the feeding of DDGS on the incidence of Brachyspira-associated colitis in pigs. One hundred 4-week-old pigs were divided …

The Shellfish Corner: How Many Shellfish Can I Grow On My Farm?, Michael A. Rice

Michael A Rice

Genetic Diversity Among Eight Dendrolimus Species In Eurasia (Lepidoptera: Lasiocampidae) Inferred From Mitochondrial Coi And Coii, And Nuclear Its2 Markers, Alexander Kononov, Kirill Ustyantsev, Baode Wang, Victor C. Mastro, Victor Fet, Alexander Blinov, Yuri Baranchikov

Victor Fet

Background: Moths of genus Dendrolimus (Lepidoptera: Lasiocampidae) are among the major pests of coniferous forests worldwide. Taxonomy and nomenclature of this genus are not entirely established, and there are many species with a controversial taxonomic position. We present a comparative evolutionary analysis of the most economically important Dendrolimus species in Eurasia. Results: Our analysis was based on the nucleotide sequences of COI and COII mitochondrial genes and ITS2 spacer of nuclear ribosomal genes. All known sequences were extracted from GenBank. Additional 112 new sequences were identified for 28 specimens of D. sibiricus, D. pini, and D. superans from five regions …

J. C. Penney: The Man, The Store And American Agriculture, David Delbert Kruger

David Delbert Kruger

Why Animal Welfarism Continues To Fail, Lori Marino

Lori Marino, PhD

Welfarism prioritizes human interests over the needs of nonhuman animals. Despite decades of welfare efforts other animals are mostly worse off than ever before, being subjected to increasingly invasive and harmful treatments, especially in the factory farming and biomedical research areas. A legal rights-based approach is essential in order for other animals to be protected from the varying ethical whims of our species.

Thinking Pigs: Cognition, Emotion, And Personality, Lori Marino, Christina M. Colvin

Lori Marino, PhD

An exploration of the cognitive complexity of Sus domesticus, the domestic pig.

Transcriptome Analysis Reveals Potential Mechanisms Underlying Differential Heart Development In Fast- And Slow-Growing Broilers Under Heat Stress, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont

Jibin Zhang

Adrift In A Sea Of Information About Sustainable Seafood: The Maine Consumer Perspective, Catherine V. Schmitt

Catherine Schmitt

The desire for a sustainable seafood industry that protects the environment and the future of fishing is certainly of interest to consumers, but even here there are conflicting standards, as Catherine Schmitt explores in this article.

Revised Alfalfa Fertilizer Recommendations For A Changing Environment Greatly Improves Profitability, Brian Lang, John E. Sawyer, Daniel Barker, Ken Pecinovsky

John E. Sawyer

During the last decade, an ever-increasing percentage of alfalfa fields in northeast Iowa have been exhibiting signs of reduced growth and vigor, resulting in declining profitability. Iowa State University Extension in northeast Iowa initiated an investigation into this problem. Sixteen research trials conducted in northeast Iowa over the next 6 years as both on-farm trials and at the northeast ISU research farm uncovered widespread sulfur deficiency problems in alfalfa production. Over 90 ISU Extension educational meetings in northeast Iowa, and extensive use of the media, communicated this issue and tools to address it to farmers and agricultural providers. Survey results …

Anthropogenic Noise Is Associated With Reductions In The Productivity Of Breeding Eastern Bluebirds (Sialia Sialis), Caitlin R. Kight, Margaret S. Saha, John P. Swaddle

John Swaddle

Although previous studies have related variations in environmental noise levels with alterations in communication behaviors of birds, little work has investigated the potential long-term implications of living or breeding in noisy habitats. However, noise has the potential to reduce fitness, both directly (because it is a physiological stressor) and indirectly (by masking important vocalizations and/or leading to behavioral changes). Here, we quantified acoustic conditions in active breeding territories of male Eastern Bluebirds (Sialia sialis). Simultaneously, we measured four fitness indicators: cuckoldry rates, brood growth rate and condition, and number of fledglings produced (i.e., productivity). Increases in environmental noise tended to …

A Sonic Net Excludes Birds From An Airfield: Implications For Reducing Bird Strike And Crop Losses, John P. Swaddle, Dana L. Moseley, Mark H. Hinders, Elizabeth P. Smith

John Swaddle

Collisions between birds and aircraft cause billions of dollars of damages annually to civil, commercial, and military aviation. Yet technology to reduce bird strike is not generally effective, especially over longer time periods. Previous information from our lab indicated that filling an area with acoustic noise, which masks important communication channels for birds, can displace European Starlings (Sturnus vulgaris) from food sources. Here we deployed a spatially controlled noise (termed a “sonic net”), designed to overlap with the frequency range of bird vocalizations, at an airfield. By conducting point counts, we monitored the presence of birds for four weeks before …

Acoustic Space Is Affected By Anthropogenic Habitat Features: Implications For Avian Vocal Communication, Caitlin R. Kight, Mark H. Hinders, John P. Swaddle

John Swaddle

Human-altered landscapes often include structural features, such as higher levels of impervious surface cover (ISC) and less vegetation, that are likely to affect the transmission of avian vocalizations. We investigated the relationships between human habitat modifications and signal transmission by measuring four acoustic parameters—persistence, reverberation, and signal-to-noise ratio (SNR) of broadcast tones, as well as absolute ambient noise level—in each of 39 avian breeding territories across an anthropogenic disturbance gradient. Using a geographic information system, we quantified the amounts of different habitat features (e.g., ISC, grass, trees) at each site; a principal component analysis was used to identify which of …

Experimental Exposure To Urban And Pink Noise Affects Brain Development And Song Learning In Zebra Finches (Taenopygia Guttata), Dominique A. Potvin, Michael T. Curcio, John P. Swaddle, Scott A. Macdougall-Shackleton

John Swaddle

Recently, numerous studies have observed changes in bird vocalizations—especially song—in urban habitats. These changes are often interpreted as adaptive, since they increase the active space of the signal in its environment. However, the proximate mechanisms driving cross-generational changes in song are still unknown. We performed a captive experiment to identify whether noise experienced during development affects song learning and the development of song-control brain regions. Zebra finches (Taeniopygia guttata) were bred while exposed, or not exposed, to recorded traffic urban noise (Study 1) or pink noise (Study 2). We recorded the songs of male offspring and compared these to fathers’ …

Blood Mercury Levels Of Zebra Finches Are Heritable: Implications For The Evolution Of Mercury Resistance, Kenton A. Buck, Claire W. Varian-Ramos, Daniel A. Cristol, John P. Swaddle

John Swaddle

Mercury is a ubiquitous metal contaminant that negatively impacts reproduction of wildlife and has many other sub-lethal effects. Songbirds are sensitive bioindicators of mercury toxicity and may suffer population declines as a result of mercury pollution. Current predictions of mercury accumulation and biomagnification often overlook possible genetic variation in mercury uptake and elimination within species and the potential for evolution in affected populations. We conducted a study of dietary mercury exposure in a model songbird species, maintaining a breeding population of zebra finches (Taeniopygia guttata) on standardized diets ranging from 0.0–2.4 μg/g methylmercury. We applied a quantitative genetics …