Life Sciences Commons^™

The Role Of An Abc Transporter As A Steroid Antagonist In Drosophila, Gregory King, Andrew Andres

Undergraduate Research Opportunities Program (UROP)

Drosophila melanogaster are holometabolous insects that have several distinct life stages including larvae and a winged adult. The larval stage is mainly a time of feeding and growth, while the adult stage is optimized for sexual reproduction and dissemination. The larval stage can itself be divided into three time periods, or instars: 1st (L1), 2nd (L2), and 3rd (L3) (Figure 1). Larval growth – both between instars and beyond – depends on specific signaling pathways controlled by a cholesterol derived steroid, 20-hydroxyecdysone (20E). Although 20E is a systemic developmental signal, little is known about the molecular details of how different …

The Use Of Chloramines To Eradicate Quagga Mussel Larvae, Trea Lacroix, Kumud Acharya

Undergraduate Research Opportunities Program (UROP)

Quagga Mussels, Dressenia bugensis, are a growing problem in the western United States, particularly in their ability to infest underwater infrastructures and clog water intake pipes and screens of power and treatment plants. Chlorine has been found to be the most effective chemical to get rid of veligers (planktonic larval form of quagga mussels) in the pipes. However, chlorine leaves a residue called trihalomethane, which is a carcinogen at higher concentrations. The purpose of this project is to test the effectiveness of an alternate chemical, chloramines (chlorine and ammonia), which leaves behind little to no residual trihalomethane. Upon experimentation with …

Ubiquitylation Of Proteins In The Frozen Wood Frog, Alexa Khan, Michael Ulrich, Kenneth Storey, Frank Van Breukelen

Undergraduate Research Opportunities Program (UROP)

Wood frogs (Rana sylvatica) are able to withstand freezing. Respiratory and cardiac activity ceases when frozen. Homeostatic functions like protein synthesis and degradation presumably must also be compromised. We investigated the fate of ubiquitin-dependent proteolysis in the freeze-thaw cycle and how that might gives clues to wood frog survival. We performed western blots for ubiquitin conjugates

Evaluating Species Responses To Climate Change Using Ecological Niche Modeling And Genetic Data, Jeanette Perry

Undergraduate Research Opportunities Program (UROP)

The current and projected future warming trends together with degradation of habitats throughout much of the Great Basin and Columbian Plateau represent real threats to many species occupying these regions. If we can determine the impacts of past climatic changes on the distribution of species, we can obtain a better understanding of the future impacts of projected climatic trends on many species in these regions. My results with the Great Basin Pocket Mouse (Perognathus parvus) may be relevant to conservation ecologists and resource managers attempting to protect several Endangered Species Act candidates, such as the pygmy cottontail (Brachylagus idahoensis). I …

Effects Of Climate Change On The Viability Of The Devils Hole Pupfish, Matthew Heuton, Stanley D. Hillyard, Frank Van Breukelen

Undergraduate Research Opportunities Program (UROP)

Devils hole is an unusual, cavernous, aquifer-fed its ideal temperature range. spring in Death Valley National Park. It is the only home to a critically endangered species, Cyprinidon diabolis, a.k.a. The Devils Hole Pupfish. C. diabolis allows us a unique look at the impact of climate change on a species that has no gene flow and no possibility of escape from its current habitat. Climate change affects ectotherms (cold-blooded animals) tremendously since their body temperature is subject to changes in the temperature of their environment. In the case of C. diabolis, they are subject to high temperatures year round, around …

Accumulation Of Polonium-210 In Different Species Of Fish In Lake Mead, Suraj Ghevarghese John, Shungmugam Nallaperumal, Vernon Hodge

Festival of Communities: UG Symposium (Posters)

Discovered by Pierre Curie and Marie Skłodowska-Curie in 1898, polonium is a chemical element with an atomic number of 84. This rare naturally occurring radioactive element is chemically similar to bismuth and tellurium, and forms in uranium ores. Polonium-210 is an naturally occurring radioactive element with a half-life of 138.376 days(1). This element is found in trace amounts in most organisms. Our research is focused on the accumulation of polonium-210 in fish that occupy in Lake Mead.

The sample species is chosen based on varying lake stratification (layers of the lake). This gives us a good idea of the difference …

The Effects Of Climate Change On Basic Animal Cell Functions, Michelle Fulbright, Andrew Andres

Festival of Communities: UG Symposium (Posters)

Increasing global temperatures during the 21st century may have detrimental effects on basic cell functions within ectothermal animals. This project aims to systematically examine the effects of climate change on secretory cargo localization through the analysis of Rab Proteins found in the model organism Drosophila melanogaster. Rab Proteins play an essential role in vesicular transport within the cell and can be genetically manipulated to monitor the biological consequences of global warming.

Effect Of Temperature And Oxygen Levels On Lactate Production In Palaemonetes Pugio, Robin Schofield, Teresa Mika, Carl Reiber

Festival of Communities: UG Symposium (Posters)

Oxygen delivery rates must keep pace with the increased metabolic rate observed in poikilothermic animals exposed to increased temperature if aerobic metabolism is to be maintained. Physical failure of the oxygen delivery system to meet these demands may be a mechanism of cardiac failure in crustaceans at temperature extremes. As part of the determination of the effect of temperature on the cardio-respiratory system, we are measuring whole animal lactate levels in grass shrimp acclimated to 20°C over temperature ranges in normoxic and hyperoxic conditions. Lactate levels are measured as an indicator of a switch from aerobic to anaerobic metabolism. Animals …

Ubiquitin Dependent Proteolysis In The Frozen Wood Frog, Michael Ulrich, Ken Storey, Frank Van Breukelen

Undergraduate Research Opportunities Program (UROP)

Wood frogs (Rana sylvatica) freeze during the winter. One might presume that homeostatic processes like protein synthesis and degradation cease during freezing. However, few data are available. In collaboration with Dr. Ken Storey (Carelton University, Canada), we are investigating the primary mode of proteolysis in wood frogs representing different points of the freeze-thaw cycle.

The Effects Of The Ground Reaction Force On The Muscle-Tendon Systems About Guinea Pig Joints, Theresa Schill, Kevin Mailland, David V. Lee

Undergraduate Research Opportunities Program (UROP)

Each limb of the limb joints of a quadruped is used differently during locomotion, and it is necessary to recognize the mechanical role each plays. The effects of the ground reaction force on the ankle, knee, and hip joints of Hartley albino guinea pigs were studied in uphill, downhill, and level situations to determine the spring-like capacity of the muscle-tendon systems that act about the limb joints.

Impact Of Historical Climate Change On The Genetic Structure Of The Great Basin Pocket Mouse, Perognathus Parvus, Ashwitha Francis, Mallory E. Eckstut, Brett R. Riddle

Undergraduate Research Opportunities Program (UROP)

The dynamics of genetic patterns, such as genetic differentiation within and between populations and the geographic distribution of genetic lineages, are often influenced by historical events (such as climatic changes) that have substantially impacted regional biodiversity (the study of phylogeography; Avise, 20001). The magnitude of genetic differentiation among populations is often increased if suitable habitat is reduced, because there is restricted migration among populations. When migration is restricted, the populations may become genetically differentiated from conspecific populations and, over time, may become independent lineages that follow distinctive evolutionary trajectories (Stebbins, 19523; Nevo & Beiles, 19892; Thomas et al., 19984). In …

3d Micro-Mr And Micro-Ct Models For Determining In-Vivo Function In The Guinea Pig Knee Model Of Osteoarthritis (Oa), Kristoffer W. Dean

Undergraduate Research Opportunities Program (UROP)

Osteoarthritis (OA) is a debilitating disease that affects an estimated 27 million adults in the United States. OA causes damage to cartilage in the joints resulting in bones that rub together. This creates pain, swelling, loss of motion of the joint, and bone spurs. As the number of adults above 65 grows (an estimated 72 million people by 2030) even more people will be at high risk for OA. It is the most common type of arthritis by far yet very little is known about it including relationships between joint structure and function. In 2004, the NIH created the Osteoarthritis …

Assessment Of Endocrine And Gonadal Condition Of Male Largemouth Bass From Lake Mead, Nevada, Reynaldo Patino, Steven L. Goodbred, Erik Orsak, Jill A. Jenkins, Michael R. Rosen

Lake Mead Science Symposium

Las Vegas Bay (LVB) of Lake Mead receives combined flows of tertiary treated wastewater effluent, urban runoff, and groundwater from the Las Vegas metropolitan area. This study examined the potential for endocrine disrupting effects of these anthropogenic inputs on male largemouth bass (Micropterus salmoides). Adult male bass were collected at two sites within Lake Mead: Overton Arm (OA, reference site), and Las Vegas Bay (LVB). Post-spawn fish were collected in July 2007 (n = 6-10 per site) and pre-spawn fish in March 2008 (n = 13 per site). Post-spawn fish were characterized by regressed testes whereas pre-spawn bass had full-grown …