Life Sciences Commons^™

The Putative Role Of Resveratrol In Sirt-1 Mediated Modulation Of The Vitamin D Pathway, Angelika Dampf Stone, Shane F. Batie, G. Kerr Whitfield, Mark R. Haussler, Peter W. Jurutka

American Association for the Advancement of Science Pacific Conference

The nuclear vitamin D receptor (VDR) modulates gene transcription in 1,25-dihydroxyvitamin D (1,25D) target tissues such as kidney, colon, and bone. The 1,25D hormone is derived from vitamin D in the skin or from the diet, and binds to and activates the VDR. We have previously shown that resveratrol, an antioxidant found in the skin of red grapes, has the ability to activate the VDR signaling pathway. Moreover, cells treated with both resveratrol and 1,25D resulted in an additive or even synergistic stimulation of VDR-mediated transcription compared to cells treated with 1,25D alone. Based on these initial results, experiments were …

Kinematic Effects Of Stride Length Perturbations On System Com Horizontal Velocity During Locomotion, Josh Bailey, Andrew Nordin, Janet Dufek, D. Lee

Interdisciplinary Research Scholarship Day

PURPOSE: To investigate the kinematic effect on the systems’ center of mass horizontal velocity in response to stride length perturbations. METHODS: Twelve healthy adults (23.1±7.71 yrs; 1.69±0.1 m; 66.82±12.6 kg; leg length 894.7±66.1 mm) performed 5 trials of preferred speed walking (PW) and running (PR)followed by 5 stride length perturbations based on percentages of leg length (60%, 80%, 100%, 120% and 140%). 3D kinematic analysis was completed using a 12-camera infrared motion capture system (Vicon, 200hz). Dependent variables computer for each condition included: center of mass horizontal velocity at the highest vertical position (COMHVhi) and at the lowest vertical position …

Examining Lower Extremity Range Of Motion And Movement Variability Chages Due To Focus Of Attention During Landing, Andrew Nordin, Janet Dufek

Interdisciplinary Research Scholarship Day

Attentional focus (AF) has been explored among a variety of motor skills providing evidence that external AF promotes automaticity and enhanced performance [6]. External focus of attention is distinguished from internal focus such that external focus is directed toward movement effect rather than body movements [6]. Movement variability provides a means of assessing functional characteristics of the neuromotor system, where normal functioning is suggested to occur within optimal limits, while excessively high or low movement variability is indicative of system dysfunction [2,4,5]. Additionally, the ability of the motor system to vary, or broadly distribute, internal loads is thought to reduce …

Step Length Perturbations Alter Variations In Center Of Mass Horizontal Velocity, Josh Bailey, Andrew Nordin, D. Lee, Janet Dufek

Interdisciplinary Research Scholarship Day

PURPOSE: The purpose of the study was to investigate the effects of SL perturbations on system COM forward velocity (vx) during walking gait. METHODS: Eight healthy adults (23.5±3.6 yrs; 1.72±0.18 m; 73.11±15.29 kg) performed 5 trials of preferred speed walking (PW) and running (PR) followed by 5 stride length perturbations based on percentages of leg length (LL: 60%, 80%, 100%, 120% and 140%). 3D kinematic analysis was completed using a 12-camera infrared motion capture system (Vicon MX T40-S, 200Hz). Data filtering and interpolation included a low pass, 4th order Butterworth filter (cutoff frequency 15Hz) and cubic (3rd order spline). Maximum …

Gender Differences In Frontal Plane Lower Extremity Kinetic Variability During Landing, Andrew Nordin

Interdisciplinary Research Scholarship Day

Investigations of human movement variability have been used as a
means of exploring neuromotor functioning, where performance variability is
thought to provide the system with flexibility and a mechanism for adaptation to
movement repetition [1,2,4,6]. Operationally, variability has been considered to
fall within optimal limits (Figure 1), while excessively high or low variability has
been implicated in injury susceptibility [1,2,4,6]. Landing has been explored
due to a high incidence of injury in athletic performance, as well as the ability
to easily control task demands through increases in landing height [3,4].
The purpose of this investigation was to evaluate changes in …

Effects Of Shoe Outsole Design And Incline On Walking Biomechanics, Sarah Horsch, Janet Dufek

Interdisciplinary Research Scholarship Day

The purpose of the study was to examine the effects of incline at foot contact of treadmill walking between rounded outsole (ROS) and traditional outsole (TOS) shoes. A rounded outsole shoe (ROS) is specifically designed with a fulcrum under the sole so that when the mass of the body is over it, the foot is forced to roll anteriorly. Traditional ROS studies have included analyses on bipedal stance single leg standing, muscle
activity during treadmill walking, kinetics during over ground walking, and kinematics after a 6-week accommodation period. Presently, there is no research comparing a ROS and a TOS at …

Interactive Video Gaming Maintains Vo2 & Hr At Current Recommended Exercise Intensities For Cardiovascular Fitness, Michael Jarrett, Shaelee Allen, Jacob E. Barkley, Janet Dufek, Paul Hafen, Krystina Moschella, James Navalta, Robert Rietjens, Richard Tandy, Antonio Santo

Interdisciplinary Research Scholarship Day

The purpose of this study was to determine the energy cost of playing select games on the Nintendo Wii for 30 contiguous minutes and whether or not the physical interaction of the participant with the gaming system and subsequent physiologic intensity diminishes with playing time.

Physically Interactive Games Increase Vo2 Above Resting Metabolic Rate, Shaelee Allen, Jacob E. Barkley, Janet Dufek, Paul Hafen, Michael Jarrett, Krystina Moschella, James Navalta, Robert Rietjens, Richard Tandy, Antonio Santo

Interdisciplinary Research Scholarship Day

The purpose of this study was to determine the energy cost, beyond resting metabolic rate (RMR), of playing select games on the Nintendo Wii for 30 contiguous minutes. Physically interactive games (i.e. Basic Run and Basic Step) increase energy expenditure above resting values compared to a sedentary game (Tanks!) and therefore may help individual’s become more active. Furthermore, Basic Run and Basic Step elicited MET values of 3.9 and 3.2, respectively, which is considered moderate-intensity exercise and could be used to meet daily recommendations for physical activity.

Effects Of Footstrike On Low Back Posture, Shock Attenuation, And Comfort In Running, Traci L. Delgado, Emilia Kubera-Shelton, Robert R. Robb, Robbin Hickman, Harvey Wallmann, Janet Dufek

Interdisciplinary Research Scholarship Day

To determine if a change from rearfoot strike (RFS) to forefoot strike (FFS) would change lumbar lordosis, influence shock attenuation, or change comfort levels in healthy recreational/experienced runners.

Immediate Effects Of Neurodynamic Versus Muscle Stretching On Hamstring Flexibility In Subjects With Short Hamstring Syndrome, Yolanda Castellote-Caballero, Marie C. Valenza, Louie Puentedura, Cesar Fernandez-De-Las-Penas, Francisco Albuquerque-Sendin

Interdisciplinary Research Scholarship Day

To compare the effectiveness of an isolated neurodynamic sciatic sliding technique (Neurodynamic group) versus static stretching (Stretching group) in comparison to a Control group receiving a placebo intervention.

Moving Forward: Preventing Water Shortage For Nevada, Sandra Blandon, Brianna Lyon

Anthropology 100: Feast and Famine In a Global World Poster Assignment

Southern Nevada is located in the arid Mojave Desert, which averages about 4 inches of rain each year. Southern Nevada gets about 90% of its water supply from the Colorado River. Seven western states and Mexico share the river. This means that The Colorado River provides water to 25 million people.

2011 Research Highlights, Yusheng Zhao

NSTec UNLV Symposium

Overview

•Center Mission and Cluster Tasks
•Recent Scientific Achievements
•Current & Future Developments

The Role Of A Transcription Factor In Regulating Rice Response To Drought Stress, Diana Ha, Liyuan A. Zhang, Jeffery Shen

Undergraduate Research Opportunities Program (UROP)

The current water shortage is a major concern in regard to our global climate change crisis. A decrease in the availability of water will have direct effects on the development of plants. Some crops, such as Oryza sativa, or commonly known as rice, requires an abundant amount of water for adequate growth. With the water shortage crisis, it will become extremely difficult to harvest such crops to meet the world’s food demand. However, many plants have evolved mechanisms for overcoming and tolerating stresses such as drought. My research focuses on studying the proteins involved with these mechanisms. The WRKY superfamily …

Investigating The Origin Of Coprolites From Three Great Basin Caves, Chelsey Vandrisse, Duane P. Moser, David Rhode

Undergraduate Research Opportunities Program (UROP)

The study of coprolites (mummified feces) is a relatively new endeavor, which enables investigations of the health and diet of ancient people and provides some of the oldest evidence to date for the human habitation in North America (2). In this project, 18 coprolites were examined from archeological digs at three Great Basin caves: the Bonneville Estates Rockshelter (UT), Hidden Cave (NV), and Top of the Terrace Rockshelter (UT). The main objectives were: 1) to verify human origin through the presence of mitochondrial DNA (mtDNA) and 2) assuming human origin, characterize intestinal microflora of Native Americans prior to European contact. …

Isolation, Characterization, And Genome Sequence Of The First Representative Of A Novel Class Within The Chloroflexi That Is Abundant In Some U.S. Great Basin Hot Springs And May Play Important Roles In N And C Cycling, Jeremy A. Dodsworth, Senthil K. Murugapiran, Jonathan Gevorkian, James Han, Tanja Woyke, Susan M. Lucas, Sam Pitluck, Len Pennacchio, Lynne Goodwin, Brian P. Hedlund

Undergraduate Research Opportunities Program (UROP)

A thermophilic, facultatively microaerophilic, heterotrophic bacterium, designated strain JAD2, was isolated from sediments of Great Boiling Spring (GBS), an ~80oC, circumneutral hot spring in the Great Basin GB). The strain grew anaerobically on yeast extract or peptone with an optimal growth temperature of 70-75oC. Growth was stimulated by addition of 0.01 atm O2 to the culture vessel headspace, but was inhibited by higher concentrations (0.2 atm). Cells of JAD2 formed non-motile filaments ranging from 10 to >300 μm in length, which typically decreased in length during stationary phase. 16S rRNA gene-targeted pyrotag sequencing and clone library data suggest that close …

Magnetosome Genes In The Gammaproteobacterium Strain Bw-2, Lucero Rivera, Denis Trubitsyn, Dennis A. Bazylinski

Undergraduate Research Opportunities Program (UROP)

Magnetotactic bacteria (MTB) biomineralize intracellular nanometer-sized, magnetic crystals surrounded by a lipid bilayer membrane known as magnetosomes. These crystals, which consist of magnetite (Fe3O4) or greigite (Fe3S4), causes the cell to align along the geomagnetic field lines as they swim, a phenomenon known as magnetotaxis. Strain BW-2 is a magnetite-producing magnetotactic bacterium isolated from Badwater Basin, Death Valley National Park (California) and is one of only two species of MTB that are known to phylogenetically belong to the Gammaproteobacteria class of the Proteobacteria phylum. The biomineralization of magnetite in magnetotactic bacteria is mediated by a series of genes that include …

Combination Of Virb Binding Site Mutations To Evaluate Collective Impact On Icsp Promoter Activity In Shigella Flexneri, Pashtana Usufuzy, Juan C. Duhart, Maria I. Castellanos, Helen Wing

Undergraduate Research Opportunities Program (UROP)

Shigella flexneri is a gram-negative, invasive bacterial pathogen that afflicts the human colonic epithelium, causing shigellosis, an illness triggering severe dysentery. The World Health Organization cites the disease burden of shigellosis near 90 million episodes and 108,000 deaths per year.

The motility and spread of Shigella is modulated by icsP, a virulence gene. The transcription factor VirB positively regulates many virulence genes encoded by the Shigella virulence plasmid. Two distal binding sites of VirB have been shown to regulate the promoter activity of icsP, despite their location of more than 1 kb upstream of the transcription start site. Five VirB …

Dna Secondary Structures And Their Contribution To Mutagenesis In B. Subtilis Stationary Phase Cells, Carmen Vallin, Holly Martin, Christian Ross, Ronald Yasbin, Eduardo Robleto

Undergraduate Research Opportunities Program (UROP)

It is widely known and accepted that the cause of many mutations in cells are generated during the replication process of actively dividing cells, however more recent research has shown that mutations also arise in non growing conditions, a phenomenon known as stationary phase mutagenesis. Much of what is known come from studies in eukaryotic and bacterial models. It has been proposed that in non~growing cells, the process of transcription plays an important role in mutagenesis. We test the hypothesis that DNA secondary structures, formed during transcription, promote mutagenesis. The transcription-generated structures are speculated to be prone to mutations by …

Inactivation Of Spo0a Gene Increases Stationary Phase Mutagenesis In Bacillus Subtilis, Denisse Reyes, Amanda Prisbrey, Holly Martin, Eduardo Robleto

Undergraduate Research Opportunities Program (UROP)

Stationary phase mutagenesis occurs when a population of cells acquires mutations conferring escape from nongrowing or stress conditions. This type of mutations is observed in nutritionally starved cells. Because the mutations occur after the onset of stress and in cells that are in non-replicative conditions, elucidating the underlying mechanisms contributes novel views to the process of evolution and apply to the formation of cancer in human cells and antibiotic resistance in microbial pathogens. Studies have shown that in Bacillus subtilis, the Mfd protein which is a transcription repair coupling factor is necessary for this phenomenon to occur. Here, we investigate …

The Role Of An Abc Transporter As A Steroid Antagonist In Drosophila, Gregory King, Andrew Andres

Undergraduate Research Opportunities Program (UROP)

Drosophila melanogaster are holometabolous insects that have several distinct life stages including larvae and a winged adult. The larval stage is mainly a time of feeding and growth, while the adult stage is optimized for sexual reproduction and dissemination. The larval stage can itself be divided into three time periods, or instars: 1st (L1), 2nd (L2), and 3rd (L3) (Figure 1). Larval growth – both between instars and beyond – depends on specific signaling pathways controlled by a cholesterol derived steroid, 20-hydroxyecdysone (20E). Although 20E is a systemic developmental signal, little is known about the molecular details of how different …

A Spatial And Temporal Analysis Of Microbial Communities In Great Boiling Spring, Nevada, U.S.A., Jessica K. Guy, Joseph P. Peacock, Jeremy A. Dodsworth, Tanja Woyke, Tijana G. Del Rio, Brian P. Hedlund

Undergraduate Research Opportunities Program (UROP)

Great Boiling Spring (GBS) is a large, circumneutral, long residence time geothermal spring in the US Great Basin. Twelve samples were taken from four different sediment sites and the planktonic community in the bulk water of GBS on up to four different dates. Microbial community composition and diversity was assessed by using a barcoded, improved universal primer set targeting the V8 portion of the 16S rRNA gene and PCR. Over 200,000 products were sequenced using the Roche 454 GS FLX Titanium System. Sediment and planktonic microbial communities were distinct with very little overlap, regardless of the sampling location or temperature. …

Biological Effects On Serpentinite Weathering, Mary H. Evert, Julie Baumeister, Elisabeth Hausrath

Undergraduate Research Opportunities Program (UROP)

Serpentinites, perhaps more than any other rock type, control the composition and evolution of the development of the surrounding ecosystems. The bulk chemistry of serpentinite rocks, high in Mg and trace elements, and low in nutrients such as Ca, K, P, and N, causes an extreme and stressful environment for ecosystems. However, the role that those serpentine ecosystems play in development of serpentine soils has not been examined.

Due to the unusual chemistry of serpentine soils, serpentine ecosystems have deeper and better-developed root systems than other ecosystems. The rhizosphere of serpentine systems, documented to produce abundant organic acids and siderophores, …

The Use Of Chloramines To Eradicate Quagga Mussel Larvae, Trea Lacroix, Kumud Acharya

Undergraduate Research Opportunities Program (UROP)

Quagga Mussels, Dressenia bugensis, are a growing problem in the western United States, particularly in their ability to infest underwater infrastructures and clog water intake pipes and screens of power and treatment plants. Chlorine has been found to be the most effective chemical to get rid of veligers (planktonic larval form of quagga mussels) in the pipes. However, chlorine leaves a residue called trihalomethane, which is a carcinogen at higher concentrations. The purpose of this project is to test the effectiveness of an alternate chemical, chloramines (chlorine and ammonia), which leaves behind little to no residual trihalomethane. Upon experimentation with …

Defining The Role Of Nras In Melanoma Maintenance, Sravya T. Challa, Sheri L. Holmen

Undergraduate Research Opportunities Program (UROP)

The incidence of melanoma has increased 600 percent over the last four decades; it is the most rapidly increasing malignancy among young people in the United States and is currently the leading cause of cancer death in women aged 25- 29. If detected early, the disease is easily treated; however, once the disease has metastasized it is largely refractory to conventional therapies and is associated with a high mortality rate. The development of cancer from a pre-malignant primary tumor to a metastatic cancer that develops at secondary sites is a multi-step process, thought to require many genetic and epigenetic events …

Cell Migration Dynamics After Alteration Of Cell-Cell Contacts In Fibrosarcoma And Glioblastoma Cell Lines, Hassan S. Rizvi, Ronald K. Gary

Undergraduate Research Opportunities Program (UROP)

Cell migration is a vital component of metastasis. In this study, our intent was to study cell migration by alteration of the Wnt/GSK-3 Pathway. Since BeSO4 is a known GSK-3 kinase inhibitor, we hypothesized that this agent would cause cell migration to decrease as a result of β-catenin stabilization. Two human cell lines, HT-1080 (fibrosarcoma) and A172 (glioblastoma), were used to observe migration levels in the presence and absence of BeSO4. Our results show that cell migration is diminished for cells that were pre-treated with BeSO4, in comparison to the untreated (control) cells.

Novel Thermophilic Cellulolytic Isolates Belonging To The Phylum Chloroflexi, Maryknoll Palisoc, Jessica K. Guy, Joseph P. Peacock, Duy C. Trinh, Jeremy A. Dodsworth, Brian P. Hedlund

Undergraduate Research Opportunities Program (UROP)

Current biofuel technologies utilize valuable foodstuffs, such as corn kernels and cane sugar, as sources of easily metabolized sugars. Microbes are used to ferment these sugars into bioethanol, a first-generation biofuel. However, in order to avoid diverting foodstuffs from the food supply, the development of second-generation biofuels technology is necessary. Second-generation biofuels are produced by converting structurally complex lignocellulosic biomass, such as agricultural and municipal wastes, to fermentable sugars or directly to biofuels.

The major technological hurdle limiting the mass production of second-generation biofuels is the difficulty in efficiently converting structurally complex lignocellulosic materials to fermentable sugars or directly to …

Ubiquitylation Of Proteins In The Frozen Wood Frog, Alexa Khan, Michael Ulrich, Kenneth Storey, Frank Van Breukelen

Undergraduate Research Opportunities Program (UROP)

Wood frogs (Rana sylvatica) are able to withstand freezing. Respiratory and cardiac activity ceases when frozen. Homeostatic functions like protein synthesis and degradation presumably must also be compromised. We investigated the fate of ubiquitin-dependent proteolysis in the freeze-thaw cycle and how that might gives clues to wood frog survival. We performed western blots for ubiquitin conjugates

Synthesis Of Chimeric Receptors Essential For Spore Germination, Christopher Yip, Christian Ross, Eduardo Robleto, Ernesto Abel-Santos

Undergraduate Research Opportunities Program (UROP)

Various species of bacteria have been reported to form an endospore, a metabolically dormant cell, during times of nutrient deficiencies and extreme stress. These said structures are outstandingly resistant to harsh chemicals, extreme temperatures, and can revert back to a metabolically active cell, through a process known as germination, when the necessary conditions are met. The rigid membrane of the endospore contains various germination (Ger) receptors which sense the external environment for necessary metabolites and germinants. Ger receptors are encoded by tricistronic operons that produce three distinct membrane proteins, the A, B, and C subunits. Although the function of the …

Evaluating Species Responses To Climate Change Using Ecological Niche Modeling And Genetic Data, Jeanette Perry

Undergraduate Research Opportunities Program (UROP)

The current and projected future warming trends together with degradation of habitats throughout much of the Great Basin and Columbian Plateau represent real threats to many species occupying these regions. If we can determine the impacts of past climatic changes on the distribution of species, we can obtain a better understanding of the future impacts of projected climatic trends on many species in these regions. My results with the Great Basin Pocket Mouse (Perognathus parvus) may be relevant to conservation ecologists and resource managers attempting to protect several Endangered Species Act candidates, such as the pygmy cottontail (Brachylagus idahoensis). I …

Regulation Of The Shigella Flexneri Icsp Gene And H-Ns Dependent Repression, Rosa Ojeda, Amanda Wigley, Dustin Harrison, Helen Wing

Undergraduate Research Opportunities Program (UROP)

The gram negative bacterium Shigella flexneri is known to cause dysentery in humans and primates. In order to help prevent the spread of shigellosis, gene regulation must be understood. Studies show that the virulence genes in S .flexneri are thermo regulated. At 30°C histone-like nucleoid structuring protein (H-NS) represses transcription of virulence genes and at 37°C VirB derepresses virulence genes. One of the genes that contribute to the virulence of S. flexneri is icsP. My project focuses on the regulation of the S. flexneri icsP gene and has two main goals. The first is to identify the sequence of H-NS …