Life Sciences Commons^™

The Effects Of Warming Rate On The Thermal Tolerance Of A Southern Population Of Striped Bass (Morone Saxatilis), James Barrett

Honors College Theses

Climate change and global warming is an ever-growing concern for our environment and the survivability of the animals which inhabit it. With water temperatures expected to rise 2-3℃ in the next century, many aquatic organisms may be limited in their habitats based on their thermal tolerance (Lleras 2019). Southern populations of striped bass (Morone saxatilis) in the Southeastern United States could be some of the hardest hit by the effects of climate change due to their unusual life history as compared to the more northern populations of striped bass. These southern striped bass do not engage in coastal …

The Effect Of Genetic Relatedness On Mate Selection And Spatial Distribution In The American Alligator, Alligator Mississippiensis, Mark Hoog

Electronic Theses and Dissertations

To date, most genetic studies on the American alligator (Alligator mississippiensis) have focused on population genetics and multiple paternity. There have not been any studies looking at how relatedness affects alligator behavior and movement. This study focused on three main questions: 1) what is the genetic diversity among alligators in this location? 2) can parentage be accurately defined among parents and offspring? and 3) how does individual relatedness affect their spatial distribution? We examined the relatedness of 174 unique individuals from the Okefenokee Swamp Park, in South Georgia, USA. In addition, we placed Telonics^® satellite tags on …

Use Of Hematological Markers To Assess Physiological Condition And Health Status In Free-Ranging Sand Tiger Sharks (Carcharius Taurus), Chestina N. Craig

Electronic Theses and Dissertations

The contents of blood can provide information about the physiological condition and health of vertebrates. This study seeks to better understand the stress physiology and blood bacteria presence of the sand tiger shark (Carcharius taurus), as sharks are known to have unique physiology and immune systems. In this study the blood metabolites glucose, lactate, and ketones (3-hydroxybuteric acid and acetoacetate), were used to understand how biotic and abiotic factors affect the acute stress response to capture and handling. Metabolite concentrations from blood plasma were analyzed using colorimetric assays. Glucose and ketones showed no significant responses to capture and …

Sustainability Practices In Aquaculture: Using Algae Turf Scrubber Biomass To Raise Black Soldier Flies As An Alternative Feed In Blue Tilapia, Oreochromis Aureus, Culture, Michelle C. Lowery

Electronic Theses and Dissertations

Since the 1980s, non-algal aquaculture has grown to encompass 49% of all seafood production in response to a growing human population and increased seafood demand (FAO, 2022). Hurdles exist to aquaculture sustainability, including dependence on wild sourced fishmeal (FM) and the impacts wastewater discharge. It takes 4-5 tons of wild forage fish to produce one ton of dry FM (Miles and Chapman, 2006) and as aquaculture is primarily conducted in earthen ponds and public open water bodies (FAO, 2022), finfish culture can have a high impact on the surrounding environment by discharging excess nutrients. This study used algae turf scrubber …

Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb

Electronic Theses and Dissertations

Nasonia is a parasitoid wasp genus that serves as an emerging model for studying speciation due to an incompatibility between nuclear and mitochondrial genomes between sister taxa. Short generation times, easy rearing in a lab setting, producing large amounts of progeny, and whole genome sequencing make Nasonia is an excellent candidate for studying incomplete reproductive isolation. Nasonia have five chromosomes and exhibit haplo-diploid sex determination in which fertilized eggs develop into diploid females and unfertilized eggs develop into haploid males. Recessive phenotypes are hidden in diploid females but are laid bare in haploid males as there is no interaction between …

Epigenetic Buffering In Introduced House Sparrows, M. Ellesse Lauer

Electronic Theses and Dissertations

Epigenetic buffering, as an environmentally induced increase in variance of epigenetic states that increases phenotypic variation to buffer populations against decreased fitness, may be a factor that resolves the genetic paradox of introduced species. DNA methylation is a molecular mechanism that could facilitate epigenetic buffering by changing in response to environmental stress. Therefore, epigenetic buffering can be detected through increased variance in DNA methylation in novel or heterogeneous environments. Introduced house sparrows (Passer domesticus) have well-documented phenotypic changes with low genetic diversity, high epigenetic diversity, and high variance in DNA methylation that provide a characteristic signature of epigenetic buffering. …

Evaluation Of Hemp Seed Meal As A Fish Meal Replacement Through Growth And Digestibility Trials In Striped Bass (Morone Saxatilis), Anthony Sample

Electronic Theses and Dissertations

Demand for seafood has continued to increase despite a stagnation in global wild-caught fisheries. Aquaculture has met this demand but suffers from numerous sustainability issues because of dependence on fish meal, which is unsustainable, sparking nutritional research into alternatives. Hemp seed meal has shown promise as an alternative due to its sustainable production and nutritional qualities. This study evaluated hemp seed meal as an alternative to fish meal through growth and digestibility trials with Morone saxatilis. A seven-week growth trial was conducted within a recirculating aquaculture system using a total of two control diets (commercial and constructed) and six …

Effects Of Synthetic Estrogen (17Α-Ethinyl Estradiol) On Male Fiddler Crab Aggression, Micayla Shirley

Honors College Theses

Pharmaceuticals, including hormones and antibiotics, are considered contaminants due to their widespread use and release into the environment. Hormones, like the synthetic estrogen used in oral contraceptives (17α-ethinylestradiol), are present in freshwater and marine systems, but with relatively unknown effects on the organisms that live there. Ethinylestradiol (EE2) accumulates in waterlogged soil (sediment) with potential to harm sediment-dwelling animals. For example, fiddler crabs (Uca pugilator) are vital members of salt marsh communities. Their burrowing adds oxygen to sediments and cycles nutrients, and they are an important food resource to birds and raccoons. Male fiddler crabs are territorial, aggressively …

Sexual Dimorphism Of Thermal Preference In Florida Scrub Lizards (Sceloporus Woodi) And Predicting Response To Climate Change In Two Rare Habitats, Sidney E. Anderson

Electronic Theses and Dissertations

The Florida Scrub Lizard (Sceloporus woodi) is endemic to Florida, where it inhabits fragments of xeric sandhill uplands including endangered long-leaf pine and sand pine scrub habitats. Lizards depend on a predictable range of temperatures to maximize their growth and productivity, and to do so, they shuttle among various thermal micro-environments. Thus, the spatial distribution of temperatures in the habitat is important. Habitats dominated by either high or low extremes of an organism’s preference are energetically costly and dangerous (less optimal), especially to gravid females. This study examines thermal preference of a near-threatened species that also inhabits increasingly rare habitats. …

Assessing Habitat Suitability Of Ribbed Mussels (Geukensia Demissa) In Georgia Salt Marshes By Examining Predicted Mussel Densities And Mussel Population Parameters, William K. Annis Jr

Electronic Theses and Dissertations

Geukensia demissa (ribbed mussel) is an ecology important bivalve that has the potential to contribute to salt marsh restoration. Understanding the factors that contribute to the distribution of mussels can help inform managers on choosing locations to optimize the survivorship of mussels in restoration projects. This study sought to model mussel densities across the coast of Georgia and to compare predicted mussel densities with mussel population parameters as means to gauge habitat suitability. Mussel densities were collected through field surveys across a range of salt marshes along the coast of Georgia and were compared with spatial data such as distance …

Using Zebrafish (Danio Rerio) To Evaluate The Use Of Food Waste Based Feed In Sustainable Small-Scale Aquaculture, Caitlin May

Electronic Theses and Dissertations

Food waste is rich in nutrients and a valuable resource, but currently its primary method of disposal in the United States (US) is unsustainable. In 2018 the US Environmental Protection Agency (EPA) estimated that the commercial, institutional, and residential sectors of the US economy wasted 63 million metric tons of food, most of which was sent to landfills. Instead, food waste could be recycled and used to supplement or replace commercial feed in aquaculture to sustainably stimulate production of fish. Zebrafish (Danio rerio) are a well characterized animal model closely related to other fish species that are important …

Habitat Use Of Wintering Henslow's Sparrows (Centronyx Henslowii) In Power Line Right-Of-Ways, Abigail W. Dwire

Electronic Theses and Dissertations

Habitat loss and degradation are the leading causes of grassland bird declines worldwide. The Henslow’s Sparrow (Centronyx henslowii; hereafter HESP) is a grassland bird species of conservation concern that has historically relied on the herbaceous ground-layer of longleaf pine savannas and similar habitats in the southeastern U.S. for food and shelter in the winter. However, due to human development, alterations of habitat, and fire suppression, only fragments of these habitats remain. Over the last decade, surveys have annually documented HESPs using power line right-of-ways (hereafter, ROWs) at several sites in the coastal plain of Georgia as alternative habitat for …

Predation Threat In A Variable Landscape: Connecting Predation Risk To Nesting Success For The Seaside Sparrow (Ammospiza Maritima Macgilivraii), Corina D. Newsome

Electronic Theses and Dissertations

Predation, the leading cause of nest-failure in birds, not only exists as a direct threat to nesting success, but may exacerbate other sources of nest mortality. Birds inhabiting Atlantic coastal marshes, such as Seaside Sparrows (Ammospiza maritima; hereafter SESP), are experiencing significant and rapid changes to their habitat, particularly sea level rise (SLR) and encroaching urbanization, that may affect the relative influence of nest predation on overall productivity. For SESPs, SLR presents an inherent threat to nest success in its potential to increase the frequency of nest flooding. In addition to this direct threat, the ability of SESPs …

The Effects Of Auditory Predator Cues On Foraging Patterns In Peromyscus Polionotus, Christina L. Sartain

Honors College Theses

Abstract:

While it is established that animals react to predator cues, little research has been done to assess its effect on foraging patterns and behavior. Foraging patterns are determined by a variety of factors associated with perceived predation risk, such as vegetation cover and moonlight exposure. Predator cues come in many forms, both direct and indirect, and are another indicator of perceived predation risk. Past research suggests that oldfield mice, Peromyscus polionotus, may react to indirect cues (i.e vegetation cover) more often than some direct cues, such as urine of a predator. Other indirect cues, such as vegetation cover and …

Effect Of Fire Suppression On Aquatic Invertebrates In Ephemeral Wetlands Embedded In Longleaf Pine Forests, Hailey E. Baker

Honors College Theses

It has been established for many years that longleaf pine forests require the ecological disturbance of fire in order to maintain a balanced ecosystem. However, a crucial part of these forests has become nearly excluded from prescribed burning. Ephemeral wetlands embedded within longleaf pine forests are a unique and dynamic seasonal habitat that provide homes, refuge, and breeding grounds for a large array of taxa. Past research suggests that fire suppression around ephemeral wetlands is causing harm to many species of amphibians and other herpetofauna, especially threatened species like the flatwoods salamander. However, other species have not been as well …

Presence Of Dirofilaria Immitis In Mosquitoes In Southeastern Georgia, Angelica C. Tumminello

Honors College Theses

Canine heartworm disease is caused by the filarial nematode Dirofilaria immitis, which is transmitted by at least 25 known species of mosquito vectors. This study sought to understand which species of mosquitoes are present in Bulloch County, Georgia, and which species are transmitting canine heartworm disease. This study also investigated whether particular canine demographics correlated with a greater risk of heartworm disease. Surveillance of mosquitoes was conducted in known heartworm-positive canine locations using traditional gravid trapping and vacuum sampling. Mosquito samples were frozen until deemed inactive, then identified by species and sex. The mosquitoes were then either dissected under …

Molecular Survey Of Helminths Infecting Ground Dwelling Birds In The Grouse Subfamily Tetraoninae, Briana M. Sesmundo

Honors College Theses

Alaskan grouse and ptarmigan (Galliformes) are important avian game species in Alaska. Interestingly, gallids harbor a fairly diverse helminth fauna, particularly cestodes, which may make birds with high parasite loads more susceptible to predation. Unfortunately, there is limited information available on the helminth fauna of Alaskan gallids, and no molecular surveys. The present study aims to develop baseline data on diversity of intestinal and subcutaneous (filariid) helminth infections in galliforms using morphological and molecular (DNA) approaches. These data can then be used to better understand changes in helminth community structure given current environmental volatility. The intestines, cloaca, liver and kidneys …

Bird Window Strikes On A College Campus: Mortality Estimates And Possible Mitigation, Antarius D. Mclain

Electronic Theses and Dissertations

Understanding the impact that human development has on wildlife populations is essential to preserving biodiversity. Bird populations are a good indicator of anthropogenic threats because they are sensitive to environmental change. Window strikes are a major source of mortality for bird populations. Studies have begun to monitor factors that cause window strikes and estimate the amount of birds killed annually by strikes. However, these estimates can be greatly affected by site dependent variables and scavenging of carcasses. My study addresses this issue by answering four questions: First, how many birds are killed annually on campus? Second, what factors complicate making …

Evaluating Elasmobranch Bycatch And Shark Depredation In The Georgia Shrimp Fishery, Matthew M. Scanlon

Electronic Theses and Dissertations

The Georgia shrimp fishery has seen a dramatic decrease in profit and productivity since the 1980’s due to a number of economic factors. Additional, yet undocumented, pressures on this fishery include interactions between foraging sharks with trawl gear. Fishermen report that sharks frequently bite nets in an attempt to prey on netted fish, resulting in large holes in the gear. Further elasmobranch interactions with trawl gear occur as bycatch; shrimp trawls represent nearly 100% of elasmobranch commercial bycatch in Georgia state waters, the species composition of which is largely unstudied. Shark interactions with nets were detailed through fishery-dependent observations on …

Predictors Of Fish Assemblage Structure And Dynamics In Atlantic Coastal Plain Streams, Rebecca Scott

Electronic Theses and Dissertations

Effective management of freshwater fishes requires a mechanistic understanding of the drivers of assemblage composition; in other words, what determines who is where and when. Stream fish assemblages are potentially influenced by environmental factors that act on multiple spatiotemporal scales, but the relative influence of these drivers may vary between geophysically distinct regions. This study sought to determine the patterns and drivers of fish taxonomic and functional assemblage composition in the coastal plain, a region possessing unique hydrologies, faunas, and physiochemical conditions. I addressed this goal using two complementary chapters, both of which utilized environmental and biotic data collected from …

An Analysis Of White-Tailed Deer, Odocoileus Virginianus, Killed In Deer Vehicle Accidents On Bulloch County, Ga Roadways, Mackenzie P. Payne 4003933

Honors College Theses

White-tailed deer, Odocoileus virginianus, are native to Georgia. With over 1.2 million individuals in the state, White-tailed deer have been implicated in a large number of motor vehicle collisions (ca. 1 million) each year. It is unknown, however, if deer are more likely to be killed: 1) if they are of particular ages (or classes), and 2) in areas with specific road characteristics. We addressed these two questions by collecting the remains of 20 White-tailed deer killed in deer vehicle collisions (“deer vehicle accidents” - DVAs) in Bulloch County, GA. When a deer was located, the road characteristics were …

The Biological Stress Response In Bluegill Sunfish (Lepomis Macrochirus) To Variations In Environmental Temperature And Dissolved Oxygen Content., Monique Kellman

Honors College Theses

With the predicted increase in global water temperature and acute hypoxic episodes, knowledge of the effects these stressors can have on local aquatic life is extremely valuable. This study thereby quantified the change in metabolic rate in Bluegill sunfish, in response to increased temperature and low dissolved oxygen concentration, by utilizing intermittent flow respirometry. Both maximum metabolic rate (MMR) and resting metabolic rate (RMR) were determined in response to variations in dissolved oxygen content, specifically > 95% O₂ and 40% O₂. Additionally, three temperature treatment groups were established, with temperatures of 20, 25 and 30°C in order to …

A Population Genetic Investigation Of The Reticulated Flatwoods Salamander (Ambystoma Bishopi) On Eglin Air Force Base, Alexander S. Wendt

Electronic Theses and Dissertations

The reticulated flatwoods salamander (RFS) is an endangered salamander with a unique life history. One of the largest known, best studied refuges for RFS is found on Eglin Air Force Base, and these RFS have been sampled and managed extensively since 2010. My thesis seeks to better understand RFS by using genetic techniques to address several unknowns, including: 1) determining the population structuring of RFS and the manageable units for species conservation, 2) estimating the size and status of populations, 3) understanding dispersal of RFS and factors that influence this, 4) exploring the breeding biology and recruitment patterns of RFS …

The Nutritional Value Of Fecal Sacs: Testing The Parental Nutrition Hypothesis, Amanda W. Burns

Electronic Theses and Dissertations

Nest sanitation is an important aspect of parental investment in birds with altricial nestlings. Passerine nestlings produce fecal matter that is encased in a mucous membrane for easy disposal. These fecal sacs are either removed from the nest and transported a distance away or they are ingested by the adult. A decline in fecal sac ingestion rates as nestlings age has been observed in several species. The parental nutrition hypothesis posits that this pattern can be attributed to increasing digestive efficiency as nestlings age. The parental nutrition hypothesis makes three predictions: fecal sac consumption rates should decrease as nestlings age, …

The Behavioral, Biochemical And Genetic Effects Of Sleep Deprivation In Zebrafish (Danio Rerio), Jade Catherine Boykin

Electronic Theses and Dissertations

Vertebrate sleep is a universal phenomenon and encompasses an array of conserved behavioral and physiological characteristics. Studies of sleep in humans have shown that sleep deprivation leads to adverse effects to human health and additional stress. Teleosts have emerged as a useful model species to study the vertebrate stress response, given its stress axis has high levels of homology with the mammals, including humans. For this study, the behavior of zebrafish (Danio rerio) subjected to acute (24 hours) and chronic (20 days) extended light exposure was monitored and whole-body cortisol was analyzed as an indication of activation of …

Parasite Infection Mediates Trait Tradeoffs In Fundulus Heteroclitus, Sarah Dunn

Honors College Theses

To be successful, an animal must eat, grow, and reproduce. With limited resources, there are tradeoffs between these critical life history parameters but the direction of the tradeoffs is largely unknown in a changing environment. To determine whether environmental context affects life-history tradeoffs, I surveyed and quantified investment into reproduction, growth, and a proxy for immunity (parasitism), in the mummichog, Fundulus heteroclitus, a common inhabitant of salt marshes in Georgia. Three salt marsh sites along coastal Georgia (Shellman Bluff, Skidaway Island, and Tybee Island) were selected using a proxy for anthropogenic disturbance (impervious surface), which also fell along a …

Relative Abundances Of The Recently Introduced Barnacles, Megabalanus Coccopoma And An Unidentified Species Of Megabalanus, In The Southeastern U.S., Jennifer L. Tyson

Honors College Theses

ABSTRACT

Megabalanus coccopoma is a prominent invasive species off the coast of Georgia. Recently, among collected samples thought to be M. coccopoma, several individuals of an unidentified species of barnacle were found. The species has been identified as a Megabalanus species, but is still unidentified to the species level. Species identification is difficult due to morphological variation, inconsistent taxonomic keys, and unknown origin. In this study I developed a method to accurately distinguish M. coccopoma from the unidentified Megabalanus sp. using sequence differences in the mitochondrial Cytochrome Oxidase I (COI) gene. This study will provide an accurate estimate of …

Epistasis In Predator-Prey Relationships, Iuliia Inozemtseva

Electronic Theses and Dissertations

Epistasis is the interaction between two or more genes to control a single phenotype. We model epistasis of the prey in a two-locus two-allele problem in a basic predator- prey relationship. The resulting model allows us to examine both population sizes as well as genotypic and phenotypic frequencies. In the context of several numerical examples, we show that if epistasis results in an undesirable or desirable phenotype in the prey by making the particular genotype more or less susceptible to the predator or dangerous to the predator, elimination of undesirable phenotypes and then genotypes occurs.

Host And Seasonal Effects On The Infection Dynamics Of Skrjabinoptera Phrynosoma (Ortlepp) Schulz, 1927, A Parasitic Nematode Of Horned Lizards, Kathryn Claire Hilsinger

Electronic Theses and Dissertations

Author's Abstract: Skrjabinoptera phrynosoma (Ortlepp) Schulz, 1927 is a common parasitic nematode of horned lizards. The life cycle of S. phrynosoma was described by Lee in 1957, but has received little attention since. The present study addressed effect of season as well as host characteristics on the infection dynamics in lizard hosts. In the Alvord Basin in southeastern Oregon, S. phrynosoma were collected from Phrynosoma platyrhinos Gerard 1852 horned lizards via stomach flushes, cloaca flushes and fecal pellet collections. Parasite load variables (number of nematodes per host, length of those nematodes, and total worm burden (ΣL)) were analyzed within three …

The Winter Ecology Of The Piping Plover (Charadrius Melodus) In Coastal Georgia, Brandon Lennon Noel

Electronic Theses and Dissertations

Author's abstract: The Piping Plover (Charadrius melodus) is a federally listed species with three distinct breeding populations, including Great Plains (threatened), Great Lakes (endangered), and Atlantic Coast (threatened), all of which winter along the Atlnatic and Gulf coasts of the United States. I studied the winter ecology of the Piping Plovers on Little St. Simons Island (LSSI), Georgia, from 2003-2006, with emphasis on the conservation significance of this site for the endangered Great Lakes population. During 2003-2004 and 2004-2005, LSSI supported up to 100 Piping Plovers during peak migration, and approximately 40 birds wintered at this site. All populations had …