Life Sciences Commons^™

The Effects Of Light Cycle Disruption On The Reactive Oxygen Species Production In Zebrafish, Talia Sanders

Honors College Theses

It has been proven that maintaining homeostasis is linked to regulation of circadian rhythms and the light cycles associated with them. Through investigation of zebrafish mitochondria subject to stress via unpredictable light cycle exposure, we previously studied the level of GSK3β protein as it relates to the health of the brain. The data has shown that when a brain undergoes stress, it results in the increased activation of GSK3β which leads to disruptions in circadian rhythm, inflammation, and cell death. Moreso, this overexpression results in reduced ATP production and lowered Complex 1 activity in the mitochondria. However, GSK3β activation is …

Breaking Up Prolonged Sitting To Improve Cardiometabolic Risk: A Comparative Analysis Of Muscle Strengthening Exercise And Treadmill Walking, Grace E. Anderson

Honors College Theses

Purpose: To examine the effectiveness of brief bouts of treadmill walking and bodyweight-based muscular strengthening exercises to mitigate the effects of sedentary behavior on blood pressure, blood glucose, cognitive performance, and perceived fatigue. Methods: Healthy young adults (n = 9) completed 3 experimental trials consisting of 4-hour sedentary periods. The trials consisted of an uninterrupted sedentary period (control), treadmill walking every 30 minutes for 5 minutes, and bodyweight-based muscular strengthening exercises every 30 minutes for 5 minutes. Blood glucose was measured at baseline and every 30 minutes thereafter. Blood pressure and fatigue were measured at baseline and every hour thereafter. …

Antimicrobial Activity Of Cat Flea (Ctenocephalides Felis) Gut Proteins On Different Days After Blood Feeding, Dhruva K. Karnik

Honors College Theses

Cat fleas (Ctenocephalides felis) are a blood-feeding ectoparasitic insect and a common domestic pest found throughout the world. Because of their reliance on host blood, fleas are exposed to blood-borne pathogens; however, the flea gut lumen is a hostile environment for microbial colonization. For example, the gut epithelia differentially express immune genes in response to feeding. In the present study, we measured the antimicrobial activity of gut proteins from cat fleas at different days after feeding (2, 5, 7, and 14). Dissected flea guts were homogenized, passed through a syringe filter, and measured in a protein assay kit. …

Examination Of Greenhouse Conditions That Influence Scale Infestation Rates On Begonia, Madeline M. Cusick

Honors College Theses

Scale insects are common pests within greenhouse and agricultural settings. I quantified scale infestation on Begonia x Richmondensis, also known as the Dragon Wing begonia, within the Armstrong Campus Greenhouse from March to November of 2023. Six different treatments accounted for variations in sunlight, humidity, and temperature within the greenhouse with the goal of determining the insect’s preferred environmental conditions. Morphological comparisons were also made between host plant Dragon Wing begonia and other begonias present within the greenhouse. Analysis indicated a significant difference in scale presence on replicate plants by zone and sunlight treatments, with more plants hosting at least …

Developing A Methodology For Evaluating Postural Control And Functional Ability In Children With Cerebral Palsy, Abigail Schoppa

Honors College Theses

Background: Cerebral Palsy (CP) is a non-progressive group of disorders that interferes with postural control and gross motor function (GMF). The purpose of this study was to develop a methodology to evaluate and link postural control and functional ability by identifying the best measures to use.

Methods: For this study, children between the ages of 12 and 22 who have CP and a Gross Motor Function Classification System level I to III were recruited. They performed a series of gross motor tests from the BOT-2 while standing on a force plate to record their center of pressure (COP) movement. Several …

Screening H3 Histone Acetylation In A Wild Bird, The House Sparrow (Passer Domesticus), Daniella Ray

Electronic Theses and Dissertations

Epigenetic mechanisms are increasingly understood to have major impacts across ecology. However, one molecular epigenetic mechanism, DNA methylation, currently dominates the literature. A second mechanism, histone modification, is likely important to ecologically relevant phenotypes and thus warrants investigation, especially because molecular interplay between methylation and histone acetylation can strongly affect gene expression. There are a limited number of histone acetylation studies on non-model organisms, yet those that exist show that it can impact gene expression and phenotypic plasticity. Wild birds provide an excellent system to investigate histone acetylation, as free-living individuals must rapidly adjust to environmental change. Here, we screen …

Investigating Division Quest: A Text-Based Adventure Narrative Game For Overcoming Difficulties Of Mitosis Learning, Amanda G. Conner

Electronic Theses and Dissertations

Mitosis learning is a source of struggle for many introductory biology students. Much of this difficulty stems from an overwhelming amount of terminology and moving pieces as well as the abstract nature of cellular processes. Game-based learning (GBL) has been used in other contexts to reduce cognitive load and provide relatable experiences upon which students can construct their mental models. However, commercial GBL is often expensive and difficult to align with individual instructor teaching preferences and learning objectives. In this study, we use a mixed methods approach to explore the use of a GBL tool made in Twine to overcome …

Chronic Training Load And Dorsiflexion Range Of Motion Throughout A Season In Professional American Soccer, Tristan L. Young

Electronic Theses and Dissertations

Soccer has an incredibly complex movement profile. Players experience various types of strains on their body from the requirement to land in awkward positions while maintaining the ability to quickly accelerate and decelerate. Dorsiflexion range of motion (DFROM) is of concern due to the movement profile and ball control that requires constant eccentric and concentric actions at the talocrural joint. With an American soccer season spanning across nine months these demands can contribute to overuse, which has been linked to restricted range of motion. Restricted DFROM and asymmetries have been identified as injury predictors, but their relationship to performance has …

Variation In The Fear Response Of Atlantic Sand Fiddler Crabs (Leptuca Pugilator) To Anthropogenic Disturbance, Katrina L. Buttram

Electronic Theses and Dissertations

Leptuca pugilator is a common inhabitant of southeast Georgia salt marshes. This study examined the boldness of Leptuca pugilator across environmental conditions and human impacts. Here, boldness was defined as time taken to reemerge from a burrow after a fear stimulus. I hypothesized that crabs would vary in their boldness based on their surroundings, reproductive timing, and sex. Field and experimental trials were conducted to isolate which factors most influence boldness. Field trials were conducted at four sites varying in human influence throughout the breeding season. During each survey, vegetation height, substrate temperature, average burrow width, burrow count, and distance …

Understanding Sport Coaches’ Responses To Injury, Ashanti Robinson

Honors College Theses

Injury is an inevitable part of any sport an athlete decides to take part in. Many things go along with injury, but one of the biggest factors is the one directly responsible for the success of the athlete: the coach. The purpose of this study was, based on a systematic review of the literature, to better understand what part coaches play in the fear of athletic injuries including their responses and the rationale for those responses. The study was conducted by cross-referencing multiple articles across different databases and systematically examining the different studies to further understand the fear of injury …

The Biobehavioral Effects Of Embryonic Exposure To Neural Inflammation And Oxidative Stress In Zebrafish, Dalton J. Anderson

Honors College Theses

The purpose of this research is to improve understanding of the neurodevelopmental effects of embryonic exposure to elevated inflammation and oxidative stress induced by the antipyretic drug acetaminophen (APAP). Our study was the first to examine the interactive effects of APAP and inflammation in zebrafish embryos and how the treatments affect brain development and larval behavior. Experimental groups of zebrafish larvae were exposed to lipopolysaccharide (LPS) to induce inflammation, APAP, or LPS + APAP and larval behavior was analyzed using Ethovision automated behavioral tracking software. We also measured changes in whole-brain Glycogen Synthase Kinase 3 Beta (GSK3B) and GSK3B phosphorylation, …

Mammalian Biodiversity On Greenways Across An Urban-To-Rural Gradient, Brooklyn C. Zwinklis

Electronic Theses and Dissertations

Large-scale urbanization is one of the leading causes of biodiversity loss on a global scale due to habitat loss and fragmentation. This has created a need to investigate ways to mitigate the damage caused by urban development, such as studying whether green spaces in urban areas can serve as means of preserving mammalian biodiversity in cities. This study specifically focused on greenways as they are understudied when compared to other green spaces, such as parks and cemeteries. Greenways are linear tracts of paved land, usually designed for human recreational activities. However, their benefits to wildlife have not been well researched. …

Exploring The Consistency Of Flow Regimes Within And Among Ecoregions Of The Southeastern United States, Frank Paul Braun Iv

Electronic Theses and Dissertations

Human manipulation of river systems has long been a known contributor to the loss of freshwater biodiversity. By accounting for environmental causes of hydrologic variation among rivers, we can better understand how ecoregion mediates flow regimes and forecast species that may be at risk. Presumably, natural variation associated with ecoregion boundaries exerts strong influence on flow regimes, and may mediate relationships between other features (e.g., land use, dam operations) and hydrology. However, such between-ecoregion variation is poorly investigated, particularly at fine spatial and temporal scales. I characterized 10 hydrologic metrics, representing the five key dimensions of the flow regime (magnitude, …

Effects Of An 8-Week Undulating Training Program On Firefighter Body Composition, Muscular Fitness, And Occupational Performance, Dylan R. Brown

Electronic Theses and Dissertations

BACKGROUND: Firefighting is a high-risk occupation that requires a certain level of health and fitness to ensure optimal occupational performance. Improving body composition by reducing fat mass and increasing muscular strength and power are two great methods for improving performance. Therefore, the purpose of this study is to examine how an 8-week undulating training program affects firefighter body composition, muscular strength/power, and occupational performance. METHODS: A total of 33 active male firefighters were recruited for this study. Prior to the start of the 8-week undulating program, all participants were pre-tested on several fitness variables including body composition, strength, …

What Are The Impacts Of Deer Browsing And Japanese Stiltgrass (Microstegium Vinenium) On Native Sapling Growth In A Residential Wetland In Southeastern Georgia?, Josie Richards

Honors College Theses

Wetlands promote biodiversity, act as climate stabilizers, and regulate water flow, yet are vulnerable to invaders. An invasive species can affect the biodiversity, abiotic conditions, and increase vulnerability of an ecosystem over time and deer browsing can actively affect new growth by removing the apical buds of young woody vegetation. Japanese stiltgrass (Microstegium vimineum), a wetland specific invader, has been shown to compete against native species for limited resources and actively crowd them out. Growth of native saplings can be further complicated by the presence of white tailed deer (Odocoileus virginianus), which browse woody vegetation and limit recruitment of trees. …

A Comparison Of Microsatellites And Single Nucleotide Polymorphisms For The Assessment Of Population Structure In The Amblyomma Maculatum Koch, 1844 Species Complex, Henrey A. Deese

Electronic Theses and Dissertations

The Gulf Coast tick, Amblyomma maculatum Koch, 1844 is widespread throughout North and Central America and is the primary vector for the emerging pathogen Rickettsia parkeri. In the U.S., the population in the Southwest (A. maculatum morphotype III) is morphologically different from the population east of the Rocky Mountains (A. maculatum morphotype II). This research tests the hypothesis that A. maculatum morphotype II and morphotype III represent distinct species through the analysis and comparison of 6 microsatellite loci and suite of 135,221 Single nucleotide polymorphisms (SNPs) generated from ddRADseq. Population genetic analysis of both microsatellites and SNPs …

Examining The Oxidative Stress Effects In Rats Exposed To 2-Aminoanthracene In Utero And High Fat Diet Three Months After, Brianna A. Lanoix

Honors College Theses

Polycyclic aromatic hydrocarbons are organic compounds that are created synthetically or found in partially burned oil, gasoline, and coal. 2-aminoanthracene (2AA) is a common example of a PAH and can affect lipid and carbohydrate metabolism and resulting in inflammatory problems and oxidative stress. The objective of the research project is to investigate oxidative stress in relation to diabetes in rats exposed to 2AA in utero and a high fat diet later in life. Pregnant dams were separated into treatment groups based on their dosages of 2AA; 0 mg/kg (control), 50 mg/kg (low), 100 mg/kg (high). During the gestation -postpartum period, …

Endogenous Antioxidant Response And Lipid Peroxidation Levels In Muscle Tissue Of The Cownose Ray (Rhinoptera Bonasus), Bailey Berry

Electronic Theses and Dissertations

Oxidative stress is the result of an imbalance between reactive oxygen species (ROS) and antioxidant response within cells. ROS generation is a natural consequence of ETC activity within the mitochondria and is associated with aerobic metabolism. Oxidative stress data on elasmobranch muscle is limited, with previous studies primarily focusing on mammalian and teleost muscle. Elasmobranch skeletal muscle is generally composed of large proportions of white muscle fibers (anaerobic), low proportions of red muscle fibers (aerobic), and low proportions of pink muscle fibers (anaerobic). The cownose ray, a benthopelagic batoid species, presents an interesting model of study because it contains relatively …

Comparative Analytics On Chilli Plant Disease Using Machine Learning Techniques, Sai Abhishta Roy Seelam

Electronic Theses and Dissertations

This thesis concerns the detection of diseases in chilli plants using machine learning techniques. Three algorithms, viz., Convolutional Neural Network (CNN), Recurrent Neural Network (RNN), and Multi-Layer Perceptron (MLP), and their variants have been employed. Chilli-producing countries, India, Mexico, China, Indonesia, Spain, the United States, and Turkey. India has the world’s largest chilli production of about 49% (according to 2020). Andhra Pradesh (Guntur) is the largest market in India, where their varieties are more popular for pungency and color. This study classifies five kinds of diseases that affect the chilli, namely, leaf spot, whitefly, yellowish, healthy, and leaf curl. A …

Remote Sensing Of Georgia Tidal Marsh Habitats Using Aerial Photography And Planetscope Satellite Imagery, Harrison M. Currin

Electronic Theses and Dissertations

Globally, tidal marshes cover about 90,800 km. Within the state of Georgia tidal marshes are primarily located behind the barrier islands and total 1,619 km². The combination of high salinity environments and daily inundation, and being dependent on river output, make these dynamic systems. Tidal marshes provide numerous ecosystem services such as carbon and nitrogen sequestration, flood control, coastal protection, and numerous biogeochemical processes. Due to their unique position, tidal marshes are under threat from sea level rise, drought, coastal development, and large-scale disturbance events. Tidal freshwater marshes are especially susceptible to these threats due to their geographic …

Use Of Hematological Markers To Assess Physiological Condition And Health Status In Free-Ranging Sand Tiger Sharks (Carcharius Taurus), Chestina N. Craig

Electronic Theses and Dissertations

The contents of blood can provide information about the physiological condition and health of vertebrates. This study seeks to better understand the stress physiology and blood bacteria presence of the sand tiger shark (Carcharius taurus), as sharks are known to have unique physiology and immune systems. In this study the blood metabolites glucose, lactate, and ketones (3-hydroxybuteric acid and acetoacetate), were used to understand how biotic and abiotic factors affect the acute stress response to capture and handling. Metabolite concentrations from blood plasma were analyzed using colorimetric assays. Glucose and ketones showed no significant responses to capture and …

Effect Of Training Modality On Intention To Exercise Among Law Enforcement Cadets Post Academy, Nicholas Hunt

Electronic Theses and Dissertations

Police academies are typically the first formalized job-task-oriented exercise training for law enforcement officers (LEOs). Research has demonstrated that cadets are at higher risk of injury during the academy. Physical training (PT) is a critical component of LEO academy efforts to build occupational skills to meet job demands. Although research is beginning to investigate the efficacy of different training modalities, more research is needed to understand the impact of academy training load and modality on occupational readiness and interest in maintaining exercise behaviors post-academy. PURPOSE: to investigate the relationships between different physical training modalities and intention to continue physical training …

The Effect Of Weekly Training Load On Match Result In American Professional Soccer, Cameron M. Horsfall

Electronic Theses and Dissertations

BACKGROUND: During a professional soccer season, training load (TL) will fluctuate due to the need to prepare for the next competition. Based on the technical staff’s daily and weekly evaluation, TL will be adjusted as they prepare the team and maximize individual performance and achieve a favorable result. Therefore, the programming of each match week (MW) TL may promote a favorable result. PURPOSE: The purpose of this study was to examine variation in daily GPS-derived TL variables based on match result using the Match Day Minus (MD-) system. This study provides descriptive TL data yet to be reported in …

Hepatic Transcriptomic Assessment Of Sprague Dawley Rats In Response To Dietary Perfluorobutane Sulfonate (Pfbs) Ingestion, Isaac Appiah

Electronic Theses and Dissertations

Perfluorobutane sulfonate (PFBS) is a short-chain PFAS that is considered to be a less toxic replacement for the rather more toxic long-chain perfluorooctane sulfonate (PFOS). Its numerous industrial applications and widespread presence in the environment have raised environmental and health concerns because of growing evidence associating adverse health effects and certain liver diseases to PFBS exposure. The study goal was to investigate whether dietary ingestion of PFBS would induce liver injuries, damage, inflammations, and oxidative stress. To achieve these goals, Sprague-Dawley rats were assigned into three PFBS dietary treatment groups (0, 50, and 100 PPM) for 11 weeks. After this …

Developing Best Practices For The Propagation Of Spartina Alterniflora For Use In Salt Marsh Restortaion, Justin Hinson

Electronic Theses and Dissertations

Coastal salt marshes are valuable ecosystems under threat from climate change and sea level rise. Living shorelines offer a promising solution, often incorporating the foundational salt marsh species Spartina alterniflora due to its ability to tolerate natural stressors and maintain sediment stability. However, research suggests that seed-based propagation protocols should be developed on a local scale due to the genetic heterogeneity within and between S. alterniflora populations. Here, we attempt to contribute to the development of one such protocol for coastal Georgia S. alterniflora.

In Fall 2021, seeds were collected bi-monthly from four marshes of varying ocean proximity and …

The Relationship Between Chronotypes And Cardiovascular Disease Risk Factors Among Law Enforcement Officers, Alyssa N. Fail

Electronic Theses and Dissertations

Much of the data associated with cardiovascular disease risk factors and circadian rhythms are done on shift workers; due to their line of work typically causing circadian disruption and sleep loss (Loef et al., 2019). Previous research incorporating chronotype and its influence on shift work has been explored; however, there is limited research focusing on law enforcement officers. Additionally, no known research has investigated the effect of chronotype on CVD risk factors among law enforcement officers. The aim of this study was to investigate the cardiovascular disease risk factors in law enforcement officers and their relationship with chronotypes. This cross-sectional …

Evaluating Blood Pressure And Hypertension Awareness In Municipal Workers In The Southeast Us, Kayden R. Mcalvin

Electronic Theses and Dissertations

The primary purpose of this study was to describe municipal workers’ blood pressure (BP) status and awareness; the secondary purpose was to examine the association between BP misconception and department. Municipal employees in a southeastern rural community (n=75) were asked to participate in a 15-minute survey, including the BASIS Hypertension Awareness survey, and blood pressure measurements to determine hypertension risk. Results show a significant difference in diastolic blood pressure (DBP) between police with a higher DBP of 87.64 ± 10.9 mm Hg and fire departments 78.98 ± 11.1 mm Hg (p=0.005), a significant difference in DBP between city hall/public works …

The Effect Of Genetic Relatedness On Mate Selection And Spatial Distribution In The American Alligator, Alligator Mississippiensis, Mark Hoog

Electronic Theses and Dissertations

To date, most genetic studies on the American alligator (Alligator mississippiensis) have focused on population genetics and multiple paternity. There have not been any studies looking at how relatedness affects alligator behavior and movement. This study focused on three main questions: 1) what is the genetic diversity among alligators in this location? 2) can parentage be accurately defined among parents and offspring? and 3) how does individual relatedness affect their spatial distribution? We examined the relatedness of 174 unique individuals from the Okefenokee Swamp Park, in South Georgia, USA. In addition, we placed Telonics^® satellite tags on …

The Effects Of Cucurbitacin B And Silmitasertib On Metastasis And Itga6 Using The Zebrafish Tumor-Xenograft, Alexandra Griffis

Electronic Theses and Dissertations

Ninety percent of cancer deaths are resultant from the metastasis of cancer cells. When cancer cells translocate through blood vessels or the lymphatic system, they may form tumors outside of their primary site. The processes of metastasis can begin quickly; after onset, metastasis is unforgiving, as it does not participate with the body’s physiological systems in an orderly way. In the past, our lab produced results indicating that a cell adhesion molecule, Integrin Alpha-6, may contribute to cancer cells' ability to metastasize. Integrins mediate interactions between the cell and the Extracellular Matrix (ECM), regulating cell attachment and cell migration. With …

Middle Savannah River: An A/R/Tographic Ecopedagogical Ethnography Experimenting With Rhizomatic Perspectives, Lisa Augustine-Chizmar

Electronic Theses and Dissertations

This research is an experiment in perspective. Using the four commonplaces (Schwab, 1978), I practiced letting the Savannah River teach me what there is to know about the water, the land, the people, and the other entities that depend on ki through artistic, ethnographic, and ecopedagogical lenses. The ethnographic findings describe the social actors that depend on ki and give a voice to the River. The a/r/tographic findings display the River on a canvas map through two hundred years using paint, clay, photography, video, abstract acrylics, and fabric. Together, these methods contribute to a unique ecopedagogical journey. This word cloud …