Open Access. Powered by Scholars. Published by Universities.®
Operations Research, Systems Engineering and Industrial Engineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Operations Research, Systems Engineering and Industrial Engineering
Prophylaxis With Human Serum Butyrylcholinesterase Protects Guinea Pigs Exposed To Multiple Lethal Doses Of Soman Or Vx, Ashima Saxena, Wei Sun, James M. Fedorko, Irwin Koplovitz, Bhupendra P. Doctor
Prophylaxis With Human Serum Butyrylcholinesterase Protects Guinea Pigs Exposed To Multiple Lethal Doses Of Soman Or Vx, Ashima Saxena, Wei Sun, James M. Fedorko, Irwin Koplovitz, Bhupendra P. Doctor
US Army Research
Human serum butyrylcholinesterase (Hu BChE) is currently under advanced development as a bioscavenger for the prophylaxis of organophosphorus (OP) nerve agent toxicity in humans. It is estimated that a dose of 200 mg will be required to protect a human against 2 × LD50 of soman. To provide data for initiating an investigational new drug application for the use of this enzyme as a bioscavenger in humans, we purified enzyme from Cohn fraction IV-4 paste and initiated safety and efficacy evaluations in mice, guinea pigs, and non-human primates. In mice, we demonstrated that a single dose of enzyme that …
Protective Efficacy Of Catalytic Bioscavenger, Paraoxonase 1 Against Sarin And Soman Exposure In Guinea Pigs, Manojkumar Valiyaveettil, Yonas Alamneh, Peter Rezk, Lionel Biggemann, Michael W. Perkins, Alfred M. Sciuto, Bhupendra P. Doctor, Madhusoodana P. Nambiar
Protective Efficacy Of Catalytic Bioscavenger, Paraoxonase 1 Against Sarin And Soman Exposure In Guinea Pigs, Manojkumar Valiyaveettil, Yonas Alamneh, Peter Rezk, Lionel Biggemann, Michael W. Perkins, Alfred M. Sciuto, Bhupendra P. Doctor, Madhusoodana P. Nambiar
US Army Research
Human paraoxonase 1 (PON1) has been portrayed as a catalytic bioscavenger which can hydrolyze large amounts of chemical warfare nerve agents (CWNAs) and organophosphate (OP) pesticides compared to the stoichiometric bioscavengers such as butyrylcholinesterase. We evaluated the protective efficacy of purified human and rabbit serum PON1 against nerve agents sarin and soman in guinea pigs. Catalytically active PON1 purified from human and rabbit serum was intravenously injected to guinea pigs, which were 30 min later exposed to 1.2 × LCt50 sarin or soman using a microinstillation inhalation exposure technology. Pre-treatment with 5 units of purified human and rabbit serum …