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Full-Text Articles in Operations Research, Systems Engineering and Industrial Engineering

Air-Exposed Microbial Fuel Cells And Screening Techniques, Justin C. Biffinger, Bradley R. Ringeisen Jan 2010

Air-Exposed Microbial Fuel Cells And Screening Techniques, Justin C. Biffinger, Bradley R. Ringeisen

U.S. Navy Research

Microbial fuel cells (MFCs) harvest energy from a wide variety of natural carbon sources to produce electricity at neutral pH and ambient temperatures. To date, standard H2/O2 PEMFC technology has yet to generate significant power under those conditions. Because most environments on earth are exposed to significant levels of oxygen, we believe the transition from sediment based MFCs to oxygen-tolerant MFCs is necessary. This transition would require both a way to simultaneously sequester the metal-reducing microbes and reduce the overall concentration of oxygen in the anode chamber. Recent work suggests that power can be generated with significant …


The Role Of Shewanella Oneidensis Mr-1 Outer Surface Structures In Extracellular Electron Transfer, Rachida A. Bouhenni, Gary J. Vora, Justin C. Biffinger, Sheetal Shirodkar, Ken Brockman, Ricky Ray, Peter Wu, Brandy J. Johnson, Eulandria M. Biddle, Matthew J. Marshall, Lisa A. Fitzgerald, Brenda J. Little, Jim K. Fredrickson, Alexander S. Beliaev, Bradley R. Ringeisen, Daad A. Saffarini Jan 2010

The Role Of Shewanella Oneidensis Mr-1 Outer Surface Structures In Extracellular Electron Transfer, Rachida A. Bouhenni, Gary J. Vora, Justin C. Biffinger, Sheetal Shirodkar, Ken Brockman, Ricky Ray, Peter Wu, Brandy J. Johnson, Eulandria M. Biddle, Matthew J. Marshall, Lisa A. Fitzgerald, Brenda J. Little, Jim K. Fredrickson, Alexander S. Beliaev, Bradley R. Ringeisen, Daad A. Saffarini

U.S. Navy Research

The ability of the metal reducer Shewanella oneidensis MR-1 to generate electricity in microbial fuel cells (MFCs) depends on the activity of a predicted type IV prepilin peptidase; PilD. Analysis of an S. oneidensis MR-1 pilD mutant indicated that it was deficient in pili production (Msh and type IV) and type II secretion (T2S). The requirement for T2S in metal reduction has been previously identified, but the role of pili remains largely unexplored. To define the role of type IV or Msh pili in electron transfer, mutants that lack one or both pilus biogenesis systems were generated and analyzed; …


Microbial Treatment Of Swine Fecal Waste To Generate Long-Chain Linear Alkanes After Fast Pyrolysis, Lisa A. Fitzgerald, C. Ziemer, S. E. Lizewski, Bradley R. Ringeisen, K. Henry, Justin C. Biffinger Jan 2010

Microbial Treatment Of Swine Fecal Waste To Generate Long-Chain Linear Alkanes After Fast Pyrolysis, Lisa A. Fitzgerald, C. Ziemer, S. E. Lizewski, Bradley R. Ringeisen, K. Henry, Justin C. Biffinger

U.S. Navy Research

Research into the generation of energy from human and animal waste has been primarily focused on gas production (especially methane and hydrogen). While there is some work concentrated on ethanol and biodiesel creation from wastes (fuels used for ground transportation primarily), increasing research interest has been recently directed toward long chain hydrocarbon production for aviation fuels. Kerosene components from swine fecal waste have been generated from four natural sources: undigested dietary lipids, host lipids excreted in feces, bacterial cell walls and products of bacterial fermentation.


Probing Electron Transfer Mechanisms In Shewanella Oneidensis Mr-1 Using A Nanoelectrode Platform And Single-Cell Imaging, Ziaocheng Jiang, Jinsong Hu, Lisa A. Fitzgerald, Justin C. Biffinger, Ping Xie, Bradley R. Ringeisen, Charles M. Lieber Jan 2010

Probing Electron Transfer Mechanisms In Shewanella Oneidensis Mr-1 Using A Nanoelectrode Platform And Single-Cell Imaging, Ziaocheng Jiang, Jinsong Hu, Lisa A. Fitzgerald, Justin C. Biffinger, Ping Xie, Bradley R. Ringeisen, Charles M. Lieber

U.S. Navy Research

Microbial fuel cells (MFCs) represent a promising approach for sustainable energy production as they generate electricity directly from metabolism of organic substrates without the need for catalysts. However, the mechanisms of electron transfer between microbes and electrodes, which could ultimately limit power extraction, remain controversial. Here we demonstrate optically transparent nanoelectrodes as a platform to investigate extracellular electron transfer in Shewanella oneidensis MR-1, where an array of nanoholes precludes or single window allows for direct microbeelectrode contacts. Following addition of cells, short-circuit current measurements showed similar amplitude and temporal response for both electrode configurations, while in situ optical imaging demonstrates …