Operations Research, Systems Engineering and Industrial Engineering Commons^™

The Role Of Shewanella Oneidensis Mr-1 Outer Surface Structures In Extracellular Electron Transfer, Rachida A. Bouhenni, Gary J. Vora, Justin C. Biffinger, Sheetal Shirodkar, Ken Brockman, Ricky Ray, Peter Wu, Brandy J. Johnson, Eulandria M. Biddle, Matthew J. Marshall, Lisa A. Fitzgerald, Brenda J. Little, Jim K. Fredrickson, Alexander S. Beliaev, Bradley R. Ringeisen, Daad A. Saffarini

U.S. Navy Research

The ability of the metal reducer Shewanella oneidensis MR-1 to generate electricity in microbial fuel cells (MFCs) depends on the activity of a predicted type IV prepilin peptidase; PilD. Analysis of an S. oneidensis MR-1 pilD mutant indicated that it was deficient in pili production (Msh and type IV) and type II secretion (T2S). The requirement for T2S in metal reduction has been previously identified, but the role of pili remains largely unexplored. To define the role of type IV or Msh pili in electron transfer, mutants that lack one or both pilus biogenesis systems were generated and analyzed; …