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FLUORESCENCE FLUCTUATION SPECTROSCOPY; CROSS-CORRELATION SPECTROSCOPY; PHOTON-COUNTING HISTOGRAM; NUCLEIC-ACIDS; LIVING CELLS; ACTIVATION; TLR9; TOLL-LIKE-RECEPTOR-9; BACTERIAL; LIFETIME
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Full-Text Articles in Nanoscience and Nanotechnology
Single Molecule In Vivo Analysis Of Toll-Like Receptor 9 And Cpg Dna Interaction, Jiji Chen, Suman Nag, Pierre-Alexandre Vidi, Joseph Irudayaraj
Single Molecule In Vivo Analysis Of Toll-Like Receptor 9 And Cpg Dna Interaction, Jiji Chen, Suman Nag, Pierre-Alexandre Vidi, Joseph Irudayaraj
Birck and NCN Publications
Toll-like receptor 9 (TLR9) activates the innate immune system in response to oligonucleotides rich in CpG whereas DNA lacking CpG could inhibit its activation. However, the mechanism of how TLR9 interacts with nucleic acid and becomes activated in live cells is not well understood. Here, we report on the successful implementation of single molecule tools, constituting fluorescence correlation/cross-correlation spectroscopy (FCS and FCCS) and photon count histogram (PCH) with fluorescence lifetime imaging (FLIM) to study the interaction of TLR9-GFP with Cy5 labeled oligonucleotide containing CpG or lacking CpG in live HEK 293 cells. Our findings show that i) TLR9 predominantly forms …