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Full-Text Articles in Nanoscience and Nanotechnology
Atomic Force Microscopy Based Dna Sensing And Manipulation, Matthew Shubert
Atomic Force Microscopy Based Dna Sensing And Manipulation, Matthew Shubert
Mechanical Engineering Undergraduate Honors Theses
Sequencing DNA provides a positive impact for the biomedical community by understanding a wide variety of applications such as human genetics, disease, and pathogens. The reason the Arkansas Micro & Nano Systems lab is involved with research in DNA sequencing is due to the current, leading industry method. Nanopore sequencing was developed by Oxford Nanopore Technology in which its sequencing method separates double stranded DNA to electrically characterize individual nucleotides traveling through a charged nanopore. Unfortunately, nanopore sequencing uses biological materials that require a shelf life and drives high cost. Therefore, the Arkansas Micro & Nano Systems lab has developed …
Atomic Force Microscopy Based Dna Analysis, Drew Creighton
Atomic Force Microscopy Based Dna Analysis, Drew Creighton
Mechanical Engineering Undergraduate Honors Theses
This report explores dry and wet scanning of a surface and DNA pickup using an AFM, as well as fluorescent staining of DNA. Dry and wet scans of DNA were obtained using a cantilever AFM tip in tapping mode. Dry scans were found to be clearer than wet scans; however, the drying process was found to decrease the thickness of DNA 2–4 times less than its original thickness. Alternately, wet scans were found to be less clear than dry scans and introduced more noise into the images obtained. Additionally, DNA kept its initial thickness during wet scanning. DNA was capable …