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Full-Text Articles in Nanoscience and Nanotechnology
Use Of Precisely Sculptured Thin Film (Stf) Substrates With Generalized Ellipsometry To Determine Spatial Distribution Of Adsorbed Fibronectin To Nanostructured Columnar Topographies And Effect On Cell Adhesion, Tadas Kasputis, Alex Pieper, Keith Brian Rodenhausen, Daniel Schmidt, Derek Sekora, Charles Rice, Eva Schubert, Mathias Schubert, Angela K. Pannier
Use Of Precisely Sculptured Thin Film (Stf) Substrates With Generalized Ellipsometry To Determine Spatial Distribution Of Adsorbed Fibronectin To Nanostructured Columnar Topographies And Effect On Cell Adhesion, Tadas Kasputis, Alex Pieper, Keith Brian Rodenhausen, Daniel Schmidt, Derek Sekora, Charles Rice, Eva Schubert, Mathias Schubert, Angela K. Pannier
Department of Biological Systems Engineering: Papers and Publications
Sculptured thin film (STF) substrates consist of nanocolumns with precise orientation, intercolumnar spacing, and optical anisotropy, which can be used as model biomaterial substrates to study the effect of homogenous nanotopographies on the three-dimensional distribution of adsorbed proteins. Generalized ellipsometry was used to discriminate between the distributions of adsorbed FN either on top of or within the intercolumnar void spaces of STFs, afforded by the optical properties of these precisely crafted substrates. Generalized ellipsometry indicated that STFs with vertical nanocolumns enhanced total FN adsorption two-fold relative to flat control substrates and the FN adsorption studies demonstrate different STF characteristics influence …
Nanotopographic Cell Culture Substrate: Polymer-Demixed Nanotextured Films Under Cell Culture Conditions, Jung Yul Lim, Christopher A. Siedlecki, Henry J. Donahue
Nanotopographic Cell Culture Substrate: Polymer-Demixed Nanotextured Films Under Cell Culture Conditions, Jung Yul Lim, Christopher A. Siedlecki, Henry J. Donahue
Department of Mechanical and Materials Engineering: Faculty Publications
Modulating physical cell culture environments via nanoscale substrate topographic modification has recently been of significant interest in regenerative medicine. Many studies have utilized a polymer-demixing technique to produce nanotextured films and showed that cellular adhesion, proliferation, and differentiation could be regulated by the shape and scale of the polymer-demixed nanotopographies. However, little attention has been paid to the topographic fidelity of the polymer-demixed films when exposed to cell culture conditions. In this brief article, two polymer-demixing systems were employed to assess topographic changes in polymer-demixed films after fibronectin (FN) extracellular matrix protein adsorption and after incubation in phosphate-buffered saline at …