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Intracellular Localisation, Geno- And Cytotoxic Response Of Poly N-Isopropylacrylamide (Pnipam) Nanoparticles To Human Keratinocyte (Hacat) And Colon Cells (Sw 480), Pratap Naha, Kunal Bhattacharya, Tiziana Tenuta, Kenneth Dawson, Iseult Lynch, Amaya Gracia, Fiona Lyng, Hugh Byrne

Articles

PNIPAM nanoparticles, with and without a covalentlylinked fluorescent label, were prepared by a free radical polymerisation technique. The cyto and genotoxicity of PNIPAM nanoparticles were analysed in two representative mammalian cell lines, SW480, a colon, and HaCaT, a dermal cell line. Physical characterisation in terms of particle size and zeta potential of the PNIPAM nanoparticles was carried out both in aqueous solution and in the appropriate cell culture media. Uptake and colocalisation of fluorescently labelled PNIPAM nanoparticles was monitored in both cell lines using confocal laser scanning microscopy. Genotoxicity analysis using the Comet assay was performed in both cell lines …