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Assembly And Function Of Myosin Ii On Ultraviolet/Ozone Patterned Trimethylchlorosilane Substrates, Madhukar B. Kolli

Theses, Dissertations and Capstones

The exploration of biomolecular motors in nanotechnological applications has generated much interest in the scientific community. Although the recent progress has been very promising, several requirements have yet to be fulfilled in order for motor proteins to be useful in nanotechnological applications. Here, we present a simple method for patterning myosin II on a microstructured surface. Our findings indicate that UV/ozone treatment can be used to alter the hydrophobicity of trimethyl-chloro-silane (TMCS) coated glass surfaces, to alter protein binding, and effectively produce localized motor activity. Taken together, these data suggest that photoreactive patterning may be useful for the selective localization …