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Full-Text Articles in Nanoscience and Nanotechnology
Investigation Of The Ms2 Bacteriophage Capsid As An Mri-Capable, Brain-Targeted Nanoparticle Platform, Stephanie M. Curley
Investigation Of The Ms2 Bacteriophage Capsid As An Mri-Capable, Brain-Targeted Nanoparticle Platform, Stephanie M. Curley
Legacy Theses & Dissertations (2009 - 2024)
Novel methods are needed to traverse the blood-brain barrier (BBB) and deliver drugs to specific targets in the brain. To this end, MS2 bacteriophage was explored as a multifunctional transport and targeting vector. The MS2 capsid exterior was modified with two different targeting moieties for delivery across the BBB and targeting specific regions of interest in the brain. Successful modification of MS2 capsids with a brain targeting peptide and NMDAR2D-targeting antibody was confirmed by immunoblotting and fluorescence detection. To measure transport efficiency of MS2 particles across an in vitro BBB model, a highly sensitive RT-qPCR protocol was developed and implemented. …
Nanotechnology & Human Stem Cells : Applications In Cardiogenesis And Neurogenesis, Martin Lyubomirov Tomov
Nanotechnology & Human Stem Cells : Applications In Cardiogenesis And Neurogenesis, Martin Lyubomirov Tomov
Legacy Theses & Dissertations (2009 - 2024)
Human stem cell research holds an unprecedented promise to revolutionize the way we approach medicine and healthcare in general, moving us from a position of mostly addressing the symptoms to a state where treatments can focus on removing the underlying causes of a condition. Stem cell research can shed light into normal developmental pathways, as we are beginning to replicate them in a petri dish and can also be used to model diseases and abnormal conditions. Direct applications can range from finding cures for single or multigene diseases to demonstrating that we can replace these genes with a normal copy. …
Bioengineering In Vitro Human Trabecular Meshwork Models For Glaucoma Therapeutic Screening, Karen Yud Torrejon
Bioengineering In Vitro Human Trabecular Meshwork Models For Glaucoma Therapeutic Screening, Karen Yud Torrejon
Legacy Theses & Dissertations (2009 - 2024)
Glaucoma refers to a group of slowly progressing eye disorders that lead to damage to the optic nerve, resulting in irreversible vision loss. Recent statistics by the World Health Organization places glaucoma as a leading cause of blindness worldwide, affecting nearly 80 million people. Lowering intraocular pressure (IOP) is currently the only effective target for therapeutic intervention in glaucoma. IOP is mostly controlled by the outflow of the aqueous humor (AH) through the trabecular meshwork (TM). The TM and adjacent endothelium of Schlemm’s canal, known as the conventional outflow-tract, control AH outflow and thus determine IOP.
Ultrastable Synergistic Tetravalent Rna Nanoparticles For Targeting To Cancers, Farzin Haque, Dan Shu, Yi Shu, Luda S. Shlyakhtenko, Piotr G. Rychahou, B. Mark Evers, Peixuan Guo
Ultrastable Synergistic Tetravalent Rna Nanoparticles For Targeting To Cancers, Farzin Haque, Dan Shu, Yi Shu, Luda S. Shlyakhtenko, Piotr G. Rychahou, B. Mark Evers, Peixuan Guo
Pharmaceutical Sciences Faculty Publications
One of the advantages of nanotechnology is the feasibility to construct therapeutic particles carrying multiple therapeutics with defined structure and stoichiometry. The field of RNA nanotechnology is emerging. However, controlled assembly of stable RNA nanoparticles with multiple functionalities which retain their original role is challenging due to refolding after fusion. Herein, we report the construction of thermodynamically stable X-shaped RNA nanoparticles to carry four therapeutic RNA motifs by self-assembly of reengineered small RNA fragments. We proved that each arm of the four helices in the X-motif can harbor one siRNA, ribozyme, or aptamer without affecting the folding of the central …