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Second-Harmonic Imaging Microscopy Of Living Cells, Paul J. Campagnola, Heather Clark, William A. Mohler, Aaron Lewis (Prof.), Leslie M. Loew
Second-Harmonic Imaging Microscopy Of Living Cells, Paul J. Campagnola, Heather Clark, William A. Mohler, Aaron Lewis (Prof.), Leslie M. Loew
Heather Clark
Second harmonic generation (SHG) has been developed in our laboratories as a high-resolution nonlinear optical imaging microscopy for cellular membranes and intact tissues. SHG shares many of the advantageous features for microscopy of another more established nonlinear optical technique: two-photon excited fluorescence (TPEF). Both are capable of optical sectioning to produce threedimensional images of thick specimens and both result in less photodamage to living tissue than confocal microscopy. SHG is complementary to TPEF in that it uses a different contrast mechanism and is most easily detected in the transmitted light optical path. It can be used to image membrane probes …