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Full-Text Articles in Mechanical Engineering
Nanomechanical Analysis Of Insulinoma Cells After Glucose And Capsaicin Stimulation Using Atomic Force Microscopy, Ruiguo Yang, Ning Xi, King Wai-Chu Lai, Bei-Hua Zhong, Carmen Kar-Man Fung, Chen-Geng Qu, Donna H. Wang
Nanomechanical Analysis Of Insulinoma Cells After Glucose And Capsaicin Stimulation Using Atomic Force Microscopy, Ruiguo Yang, Ning Xi, King Wai-Chu Lai, Bei-Hua Zhong, Carmen Kar-Man Fung, Chen-Geng Qu, Donna H. Wang
Department of Mechanical and Materials Engineering: Faculty Publications
Aim: Glucose stimulates insulin secretion from pancreatic islet β cells by altering ion channel activity and membrane potential in the β cells. TRPV1 channel is expressed in the β cells and capsaicin induces insulin secretion similarly to glucose. This study aims to investigate the biophysical properties of the β cells upon stimulation of membrane channels using an atomic force microscopic (AFM) nanoindentation system.
Methods: ATCC insulinoma cell line was used. Cell stiffness, a marker of reorganization of cell membrane and cytoskeleton due to ion channel activation, was measured in real time using an integrated AFM nanoindentation system. Cell height that …