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Full-Text Articles in Mechanical Engineering
An Electronic Pollen Detection Method Using Coulter Counting Principle, Zheng Zhang, Jiang Zhe, Santanu Chandra, Jun Hu
An Electronic Pollen Detection Method Using Coulter Counting Principle, Zheng Zhang, Jiang Zhe, Santanu Chandra, Jun Hu
Dr. Jiang Zhe
A method for detecting and counting pollen particles based on Coulter counting principle is presented. This approach also provides information on the size and surface charges of the micro particles, allowing for preliminary differentiation of pollens from other micro particles. Three samples are studied: polymethyl methacrylate particles, tree pollens from Juniperus Scopulorum and grass pollens from Secale Cerale. The samples, suspended in diluted KCl aqueous solutions in an electrochemical cell, were allowed to pass through a microchannel and the conductance of the microchannel was sampled with a Gamry®Potentiostat. The changes in the conductance due to the passing of the micro …
Novel Quantitative Macro Biomolecule Analysis Based On A Micro Coulter Counter, Y Han, H. Wu, F. Liu, Gang Cheng, Jiang Zhe
Novel Quantitative Macro Biomolecule Analysis Based On A Micro Coulter Counter, Y Han, H. Wu, F. Liu, Gang Cheng, Jiang Zhe
Dr. Jiang Zhe
We demonstrate quantitative biomolecule analysis using a micro coulter counter. Specific binding between antibody functionalized microparticles and target biomolecule cause large aggregates of microparticles. The micro coulter counter was employed to measure aggregation ratio, ratio of the aggregate counts to the total particle counts. Goat anti-rabbit IgG, used as a model biomarker, were tested in this paper. The experiment results showed that the aggregation ratio increases with the increasing biomolecule concentration, and the detectable concentration range from 16 to 160 ng/ml was achieved.