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Resistive Pulse Sensing Of Protein Unfolding And Transport In Solid-State Nanopores, Jugal Saharia
Resistive Pulse Sensing Of Protein Unfolding And Transport In Solid-State Nanopores, Jugal Saharia
Mechanical Engineering Research Theses and Dissertations
Solid-state nanopore sensors have attracted considerable attraction as a tool for solution-based single-molecule studies and have been successfully utilized for characterization of biomolecules such as nucleic acids, proteins, glycans, viruses, etc. Among these, characterization of proteins has been more challenging due to their charge heterogeneity and the complex energy landscape associated with different protein conformations. Presented in this thesis is the fabrication of solid-state nanopores and their application for characterizing proteins and understanding their transport through nanopores. Fabrication of nanometer-sized pores in SixNy membranes was achieved using the conventional controlled dielectric breakdown method as well as a …