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California Polytechnic State University, San Luis Obispo
Articular cartilage; Growth and remodeling; Cartilage matrix; Glycosaminoglycan; Collagen crosslinks; ?-Aminopropionitrile
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Full-Text Articles in Mechanical Engineering
Cartilage Growth And Remodeling: Modulation Of Balance Between Proteoglycan And Collagen Network In Vitro With Β-Aminopropionitrile, Anna Asanbaeva, Koichi Masuda, Eugene J-M.A. Thonar, Stephen M. Klisch, Robert L. Sah
Cartilage Growth And Remodeling: Modulation Of Balance Between Proteoglycan And Collagen Network In Vitro With Β-Aminopropionitrile, Anna Asanbaeva, Koichi Masuda, Eugene J-M.A. Thonar, Stephen M. Klisch, Robert L. Sah
Mechanical Engineering
Objective
To examine the effect of β-aminopropionitrile (BAPN), an inhibitor of lysyl oxidase, on growth and remodeling of immature articular cartilage in vitro.
Design
Immature bovine articular cartilage explants from the superficial and middle layers were cultured for 13 days in serum-containing medium with or without BAPN. Variations in tissue size, accumulation of proteoglycan and collagen (COL), and tensile mechanical properties were assessed.
Results
The inclusion of serum resulted in expansive tissue growth, stimulation of proteoglycan and COL deposition, and a diminution of tensile integrity. Supplementation of medium with BAPN accentuated this phenotype in terms of a further increase in …