Mechanical Engineering Commons^™

Cryopreservation Of Reproductive Cells And Tissues, Guanglei Li

LSU Master's Theses

Cryopreservation of reproductive cells and tissues is a useful technique to deliver intact genomes, maximize the distribution of favorable genes, control disease spread. It is widely applied to many fields such as biology, medicine, ecology and agriculture. To optimize the cryopreservation procedure, the biophysical responses of reproductive tissues at different cooling conditions and in different mediums must be clearly understood. Here we studied the biophysical response during freezing of: 1) bovine sperm frozen at a cooling rate of 20 ˚C/min in three media: without cryopreservation agents(CPAs), with 0.7 M glycerol, and with 0.7 M glycerol along with 1.5 mg/ml of …