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Full-Text Articles in Mechanical Engineering
Intracellular Ice Formation In Adult Stem Cells In The Presence Of Polyvinyl Pyrrolidone, Avishek Guha
Intracellular Ice Formation In Adult Stem Cells In The Presence Of Polyvinyl Pyrrolidone, Avishek Guha
LSU Master's Theses
The main objective of this work was to assess the effect of 10% (w/v) polyvinylpyrrolidone (PVP) on the pattern of intracellular ice formation (IIF) in human adipose tissue derived adult stem cells (ASCs) in the absence of serum and other cryoprotective agents (CPAs). Passage 1 (P1) ASCs were cultured, washed and suspended in either 1x PBS (Phosphate Buffered Saline) or 10% w/v solution of PVP in 1x PBS. The freezing experiments were carried out using a fluorescence microscope equipped with a Linkam™ cooling stage using two different temperature/time cooling protocols. Both the cooling protocols had a common cooling ramp: cells …
Freezing Processes In Cell Suspensions Evaluated Using Cryomicroscopy, Tathagata Acharya
Freezing Processes In Cell Suspensions Evaluated Using Cryomicroscopy, Tathagata Acharya
LSU Master's Theses
This thesis aims at evaluating the freezing response of three different cell types, Pacific Oyster embryos, Jurkats and Helas, using the technique of cryomicroscopy. The choice of cells was primarily based on supporting ongoing research work at the Bioengineering Laboratory, Department of Mechanical Engineering at Louisiana State University in Baton Rouge. On a secondary basis, the cells were chosen based on their contrasting nature. While Pacific Oyster being a favorite food in USA calls for successful techniques of cryopreservation of their embryos in order to keep up with the growing demand, Jurkat and HeLa are undesired malignant human cells that …
Cryopreservation Of Reproductive Cells And Tissues, Guanglei Li
Cryopreservation Of Reproductive Cells And Tissues, Guanglei Li
LSU Master's Theses
Cryopreservation of reproductive cells and tissues is a useful technique to deliver intact genomes, maximize the distribution of favorable genes, control disease spread. It is widely applied to many fields such as biology, medicine, ecology and agriculture. To optimize the cryopreservation procedure, the biophysical responses of reproductive tissues at different cooling conditions and in different mediums must be clearly understood. Here we studied the biophysical response during freezing of: 1) bovine sperm frozen at a cooling rate of 20 ˚C/min in three media: without cryopreservation agents(CPAs), with 0.7 M glycerol, and with 0.7 M glycerol along with 1.5 mg/ml of …