Mechanical Engineering Commons^™

Numerical And Experimental Investigation Of Transport Processes In Biological Systems, Yimeng He

LSU Master's Theses

This thesis focuses on optimization techniques for transport process during the cryopreservation of biological systems. In the first part of this thesis, a well established shape independent Differential Scanning Calorimeter (DSC) technique was used to measure the dehydration response during freezing of Pacific oyster sperm cells. Volumetric shrinkage during freezing of diploid and tetraploid pacific oyster sperm cell suspensions was obtained at cooling rates of 5 and 20 ˚C/min in the presence of extracellular ice and CPAs. By fitting a model of water transport to the experimentally obtained volumetric shrinkage data the best fit membrane permeability parameters (L_pg and …