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Full-Text Articles in Mechanical Engineering
Cryopreservation Of Reproductive Cells And Tissues, Guanglei Li
Cryopreservation Of Reproductive Cells And Tissues, Guanglei Li
LSU Master's Theses
Cryopreservation of reproductive cells and tissues is a useful technique to deliver intact genomes, maximize the distribution of favorable genes, control disease spread. It is widely applied to many fields such as biology, medicine, ecology and agriculture. To optimize the cryopreservation procedure, the biophysical responses of reproductive tissues at different cooling conditions and in different mediums must be clearly understood. Here we studied the biophysical response during freezing of: 1) bovine sperm frozen at a cooling rate of 20 ˚C/min in three media: without cryopreservation agents(CPAs), with 0.7 M glycerol, and with 0.7 M glycerol along with 1.5 mg/ml of …
Tissue Interactions With Lasers And Liquid Nitrogen: A Novel Cryopreservation Method, Deepak Kandra
Tissue Interactions With Lasers And Liquid Nitrogen: A Novel Cryopreservation Method, Deepak Kandra
LSU Master's Theses
The importance of the imposed cooling rate in cryopreserving native cells and tissues has been long recognized in the field of cryobiology. When biological tissues are subjected to cooling rates in excess of thousands of degree C per minute, the characteristic structural and physical manifestations of the ice formed are such that the traditional damage due to ice formation at lower cooling rates are suppressed. Hence, achieving high cooling rates in tissues and cells of biologically relevant sizes (mm’s and cm’s) has been a long standing research problem. In the present study, we present a novel technique to achieve high …