Open Access. Powered by Scholars. Published by Universities.®

Mechanical Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 2 of 2

Full-Text Articles in Mechanical Engineering

The Development Of A Primer Payload With Microparticles For Uti Pathogen Identification Using Polythymidine- Modified Lamp Primers In Droplet Lamp, Jonas Otoo May 2023

The Development Of A Primer Payload With Microparticles For Uti Pathogen Identification Using Polythymidine- Modified Lamp Primers In Droplet Lamp, Jonas Otoo

KGI Theses and Dissertations

Nucleic acid amplification tests (NAATs) are among the diagnostic tests with the highest sensitivity and specificity. However, they are more complex to develop than other diagnostic tests such as biochemical tests and lateral flow immunoassay tests. Polymerase chain reaction (PCR) is the gold standard for NAATs. PCR requires thermal cycling to achieve clonal amplification of the target pathogen DNA for diagnosis. Thermal cycling poses a challenge in the development of PCR diagnostics for point-of-care (POC) settings. Loop-mediated isothermal amplification (LAMP) offers an isothermal method for NAATs diagnostics. The advancement of the microfluidics field significantly enhances the development of LAMP diagnostics …


Creativity, Craftsmanship, And Connection: Large-Format Sculpture Design, Jacqueline L. Puga, Gordon Hoople Feb 2023

Creativity, Craftsmanship, And Connection: Large-Format Sculpture Design, Jacqueline L. Puga, Gordon Hoople

The STEAM Journal

Artistry is a concept that is not usually explored in engineering yet is an invaluable skill that touches everything from product design to systems thinking. This past summer I developed conceptual designs for a large format sculpture that required connecting engineering knowledge with artistic vision. The initial phase required constant inspiration and creativity. The first step was to look at previous sculptures showcased throughout the world, such as at venues like “Burning Man,” to understand the possibilities or limitations of the space provided. Sketching varied and numerous ideas was essential in our design process. Next, we took our favorite ideas …