Engineering Science and Materials Commons^™

Quantitative Macro- And Microscale Methods For Characterizing Cell Viability, Julianne Forman Audiffred

LSU Doctoral Dissertations

The goal of this study is to combine molecular and microdevice methods to characterize and quantify viability of single mammalian cells. Fluorescent-based assays were optimized for adherent HeLa and suspension Jurkat cells and were used as a tool for validation of a microfabricated diagnostic device. Cell and substrate/surface interactions were considered for designing a microfluidic device that can be used to characterize cell viability for quantitative biomedical and cell biology applications, which require label-free, real-time monitoring of cells. Several interdisciplinary methods are employed to evaluate electrical impedance differences between live and dead Jurkat cells in a microfluidic device. Biological Micro-Electro-Mechanical …