Electrical and Computer Engineering Commons^™

Preserved Enzymatic Activity Of Glucose Oxidase Immobilized On Unmodified Electrodes For Glucose Detection, Gang Wang, Ngee Mei Thai, Siu-Tung Yau

Electrical and Computer Engineering Faculty Publications

Glucose sensing electrodes have been realized by immobilizing glucose oxidase (GOx) on unmodified edge plane of highly oriented pyrolytic graphite (epHOPG) and the native oxide of heavily doped silicon (SiO₂/Si). Both kinds of electrode show direct interfacial electron transfer due to the redox process of the immobilized GOx. The measured formal potential of the redox process agrees with that of the native enzyme, suggesting that the immobilized GOx has retained its enzymatic activity. The electron transfer rates of the GOx immobilized electrode are 2s⁻¹ for GOx/epHOPG electrode and 7.9s⁻¹ for GOx/SiO₂/Si electrode, which …