Civil and Environmental Engineering Commons^™

Biofilm Microbial Community Structure In An Urban Lake Utilizing Reclaimed Water, Tianzhi Wang, Yunkai Li, Tingwu Xu, Naiyang Wu, Mingchao Liang, Paul Hynds

Articles

Analyses of biofilm community structure may potentially be employed for aquatic ecosystem health assessment, however, to date, biofilm diversity within urban lakes using reclaimed water has not been examined. Accordingly, the microbial community diversity and structure of biofilms from the surface of multiple matrices with varying roughness (0.1, 1.0 and 10.0 μm) were characterized using a suite of molecular techniques including scanning electron microscopy, genetic fingerprinting and phospholipid-derived fatty acid analyses. Samples were largely comprised of inorganic particles, algae and numerous bacterial species; 12 phospholipid-derived fatty acid (PLFA) types were identified, significantly less than typically associated with sewage. Both growth …