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Life Sciences

Theses/Dissertations

2023

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Full-Text Articles in Civil and Environmental Engineering

Exploring Soil Microbial Dynamics In Southern Appalachian Forests: A Systems Biology Approach To Prescribed Fire Impacts, Saad Abd Ar Rafie Dec 2023

Exploring Soil Microbial Dynamics In Southern Appalachian Forests: A Systems Biology Approach To Prescribed Fire Impacts, Saad Abd Ar Rafie

Doctoral Dissertations

Prescribed fires in Southern Appalachian forests are vital in ecosystem management and wildfire risk mitigation. However, understanding the intricate dynamics between these fires, soil microbial communities, and overall ecosystem health remains challenging. This dissertation addresses this knowledge gap by exploring selected aspects of this complex relationship across three interconnected chapters.

The first chapter investigates the immediate effects of prescribed fires on soil microbial communities. It reveals subtle shifts in porewater chemistry and significant increases in microbial species richness. These findings offer valuable insights into the interplay between soil properties and microbial responses during the early stages following a prescribed fire. …


Evaluating The Impact Of Oyster Reef Breakwaters On Hydrodynamics, Morphodynamics, And Sediment Transport Using Xbeach, Tabassum Islam Dec 2023

Evaluating The Impact Of Oyster Reef Breakwaters On Hydrodynamics, Morphodynamics, And Sediment Transport Using Xbeach, Tabassum Islam

<strong> Theses and Dissertations </strong>

Alabama Port is a unique marsh and sandy beach shoreline. Since the 1950s, substantial erosion has occurred along this shoreline due to storms, wind-generated waves, and ship wakes. This research focuses on evaluating the effect of oyster reef breakwaters on wave height attenuation and sediment stabilization through numerical modeling (XBeach) under three different synthetic storm scenarios coupled with three unique sea level rise projections near Alabama Port. This has been accomplished by comparing a with and without project condition using XBeach in a two-dimensional (2D) mode by analyzing wave height, velocity, and cumulative bed-level changes at the study site. The …


Hurricanes And Tropical Storms’ Impact On Water Quality In Lake Okeechobee, Florida, Daniela Vasquez Diaz Oct 2023

Hurricanes And Tropical Storms’ Impact On Water Quality In Lake Okeechobee, Florida, Daniela Vasquez Diaz

USF Tampa Graduate Theses and Dissertations

Hurricanes are natural events with catastrophic outcomes, while climate change's effects on their intensity and frequency remain discussed. However, it is crucial to analyze these through models and statistics to understand their behavior and impact on the basins. Particularly in lakes on tropical coasts since disruptive events such as hurricanes tend to be highly affected. Lake Okeechobee is a unique lake located in Florida and one of the largest freshwater lakes. The lake and some of its significant stations are the locations chosen to perform the investigation. The main focus of the investigation is determining the effect that hurricanes have …


Investigation Into The Load Transfer Mechanism Of Trees To Develop An Innovative Foundation Configuration Using 3d Finite Element Modeling, Kaleb Boland Aug 2023

Investigation Into The Load Transfer Mechanism Of Trees To Develop An Innovative Foundation Configuration Using 3d Finite Element Modeling, Kaleb Boland

All Theses

This study was inspired by a tree’s reliance on its root system that enables it to withstand a natural disaster, such as an extreme hydroclimatic event or hurricane. This curiosity has led to an investigation into what makes this possible, developing a new and innovative foundation configuration for infrastructure systems, and a new possible construction material and method. This study provides results on a material that does not have conventional testing procedures or standards: a tree root. The trees studied are common to the upstate of South Carolina, which are Pine, Sweetgum, and White Oak. According to the results, on …


Using Machine Learning And Distributed Hydrologic Modeling To Predict Soil Texture, Surface Soil Moisture And Evapotranspiration In Jornada Experimental Range, Southwestern U.S., Jorge Andres Mayo Jul 2023

Using Machine Learning And Distributed Hydrologic Modeling To Predict Soil Texture, Surface Soil Moisture And Evapotranspiration In Jornada Experimental Range, Southwestern U.S., Jorge Andres Mayo

Open Access Theses & Dissertations

In water-limited ecosystems, detailed knowledge of the soil, vegetation, and atmosphere interactions is critical to understand the processes that control the partitioning of energy, water fluxes, and biogeochemical cycles within the critical zone. This Master's thesis is divided into two main contributing sections. The first, is on the use of machine learning to reconstruct missing soil type information, and the second, on the calibration and validation of a physically-based distributed hydrological model to estimate soil moisture and evapotranspiration within the Jornada Experimental Range of the U.S. in southern New Mexico. For the first contribution, three explainable, shallow machine-learning techniques are …


Resource Recovery From Crew Metabolic Wastes In Lunar And Martian Habitats: Reuse Of Effluent From An Anaerobic Membrane Bioreactor Treating Fecal Organic Matter As Fertilizer Source For Plants, Alexandra A. Smith Jun 2023

Resource Recovery From Crew Metabolic Wastes In Lunar And Martian Habitats: Reuse Of Effluent From An Anaerobic Membrane Bioreactor Treating Fecal Organic Matter As Fertilizer Source For Plants, Alexandra A. Smith

USF Tampa Graduate Theses and Dissertations

As humans set their sights on exploration of the Moon and Mars human focused challenges like providing potable water, supplying nutritious food to the astronaut crew, and managing their waste present themselves. There are no readily available resources on the Moon and Mars, therefore nothing should be considered unusable including the organic wastes (i.e., feces and urine) produced by the crew. Fecal matter is not currently recycled and is treated as solid waste. After collection, it is discarded out of the International Space Station where it is incinerated upon re-entry. This approach will not work on the Moon’s surface. There …


Rattus Norvegicus As A Biological Detector Of Clandestine Remains And The Use Of Ultrasonic Vocalizations As A Locating Mechanism, Gabrielle M. Johnston May 2023

Rattus Norvegicus As A Biological Detector Of Clandestine Remains And The Use Of Ultrasonic Vocalizations As A Locating Mechanism, Gabrielle M. Johnston

Master's Theses

In investigations, locating missing persons and clandestine remains are imperative. One way that first responder and police agencies can search for the remains is by using cadaver dogs as biological detectors. Cadaver dogs are typically used due to their olfactory sensitivity and ability to detect low concentrations of volatile organic compounds produced by biological remains. Cadaver dogs are typically chosen for their stamina, agility, and olfactory sensitivity. However, what is not taken into account often is the size of the animal and the expense of maintaining and training the animal. Cadaver dogs are typically large breeds that cannot fit in …


Trace Dna Detection Using Diamond Dye: A Recovery Technique To Yield More Dna, Leah Davis May 2023

Trace Dna Detection Using Diamond Dye: A Recovery Technique To Yield More Dna, Leah Davis

Master's Theses

This study aspires to find a new screening approach to trace DNA recovery techniques to yield a higher quantity of trace DNA from larger items of evidence. It takes the path of visualizing trace DNA on items of evidence with potential DNA so analysts can swab a more localized area rather than attempting to recover trace DNA through the general swabbing technique currently used for trace DNA recovery. The first and second parts consisted of observing trace DNA interaction with Diamond Dye on porous and non-porous surfaces.

The third part involved applying the Diamond Dye solution by spraying it onto …


The Perceptions And Effects Of Nutrition On Worker Health And Productivity In Construction, Matthew Scott Howell Mar 2023

The Perceptions And Effects Of Nutrition On Worker Health And Productivity In Construction, Matthew Scott Howell

Construction Management

Construction workers often have poor dietary habits due to poor nutrition education, lack of nutritious food options, and social stigma. These habits often lead to health problems like obesity, diabetes, and heart disease which can decrease productivity and put workers at risk of on-site injury. This preliminary study examines how nutrition impacts worker safety and productivity. A survey was distributed to six students involved in construction work for Cal Poly’s Residential Construction course. This survey identified their eating habits and inhibitors for healthy eating. Two meals, one with high nutritional value and one with low nutritional value, were given to …


Building Artificial Reefs From Recycled Construction Materials: A Feasibility Study, Nicholas H. Lew Mar 2023

Building Artificial Reefs From Recycled Construction Materials: A Feasibility Study, Nicholas H. Lew

Construction Management

Naturally occurring reefs are some of the world’s most biologically diverse ecosystems formed by jagged rocks tucked slightly below sea level. In recent years global warming began to pose a major threat to many reef habitats. Most relevant is the increase in surface seawater temperatures that cause coral to bleach, taking away major food sources for larger marine species. Researchers have combated this by deploying artificial reefs in substitution for naturally formed limestone rock formations in order to promote the expansion of coastal habitats. This project specifically aims to utilize construction waste towards the production of artificial reefs, effectively upcycling …


The Kinetics Of Biocontaminant Inactivation: A Comparative Study With Bacillus Globigii And Bacillus Anthracis, Leigh M. Durden Mar 2023

The Kinetics Of Biocontaminant Inactivation: A Comparative Study With Bacillus Globigii And Bacillus Anthracis, Leigh M. Durden

Theses and Dissertations

Bacillus anthracis has been a biological threat for decades and after the 2001 terrorist attack via the U.S. Postal Service where five people died, there was heightened awareness and inquiry on the subject (Goel, 2015; Greenberg et al., 2010; Nicholson & Galeano, 2003; Spotts Whitney et al., 2003). The health risk to humans makes this biological agent, a Tier 1 agent and one of the most likely to be used in an attack (Anthrax as a Bioterrorism Weapon | CDC, n.d.; Driks, 2009; Rose & Rice, 2014). B. anthracis is one of most resistant and potent biological warfare agents as …


Optimizing Sample Collection And Data Interpretation For Effective Wastewater-Based Epidemiology In Combined Sewer Systems, Christopher Allen Anderson Jan 2023

Optimizing Sample Collection And Data Interpretation For Effective Wastewater-Based Epidemiology In Combined Sewer Systems, Christopher Allen Anderson

Graduate Theses, Dissertations, and Problem Reports

COVID-19 has spurred growth in the science surrounding wastewater-based epidemiology (WBE) pertaining to the detection of severe acute respiratory virus 2 (SARS-CoV-2) in waste streams as an early warning signal for public health. However, the highly variable wastewater environment has made it difficult to standardize an approach for sampling and analysis, especially in locations using combined sewer infrastructure. This study addresses knowledge gaps of WBE via three specific aims: (1) to compare diurnal fluctuations of SARS-CoV-2 and the human fecal indicator, pepper mild mottle virus (PMMoV) in wastewater treatment plant (WWTP) influent samples collected during dry versus wet weather conditions; …


Development Of A Framework To Support Community-Scale Nutrient Recovery For Local Crop Fertilization And Production, Scott A. Lopez Jan 2023

Development Of A Framework To Support Community-Scale Nutrient Recovery For Local Crop Fertilization And Production, Scott A. Lopez

Graduate Theses, Dissertations, and Problem Reports

Nutrient and resource recovery (NRR) has become increasingly crucial as regions face deteriorating sanitation infrastructures, limited support, and growing environmental challenges. Implementing region-specific NRR technologies and systems could provide effective circular economy insights into addressing these challenges. Because of the multidisciplinary challenges associated with the design and implementation of NRR strategies, various government and local decision-makers need to collaborate effectively in the decision-making process. Structured decision-making (SDM) methodologies are practical when determining the most appropriate NRR strategy for nutrient-rich waste streams. However, applications of computational SDM limited because of the vast amounts of data that are needed to analyze the …


Metagenomic Insights Into The Full-Scale Anaerobic Digestion Of Cow Manure And Pre-Treated Food Waste, Amy Camille Decola Jan 2023

Metagenomic Insights Into The Full-Scale Anaerobic Digestion Of Cow Manure And Pre-Treated Food Waste, Amy Camille Decola

Graduate College Dissertations and Theses

Anaerobic digestion provides many benefits, such as diverting organic waste from landfills, promoting nutrient recovery, reducing greenhouse gas emissions, and producing renewable energy. A complex community of microorganisms is responsible for the anaerobic digestion process that converts organic matter into biogas, a renewable energy source. The start-up phase of an anaerobic digester is considered a crucial point in solidifying the core microbial community that will ensure a stable anaerobic digester. A full-scale anaerobic digester that co-digests cow manure and pre-treated food waste from a hydrolysis tank was studied from start-up through stable performance in order to better understand the dynamics …


Food Waste Storage Gaseous Emissions Detection And Quantification Using Infrared Spectroscopy, Ryley A. Burton-Tauzer Jan 2023

Food Waste Storage Gaseous Emissions Detection And Quantification Using Infrared Spectroscopy, Ryley A. Burton-Tauzer

Cal Poly Humboldt theses and projects

A growing interest in sustainable waste management and the implementation of new policies have prompted a shift towards alternative resource recovery methods for organic waste, including food waste. To effectively assess alternative food waste treatment scenarios, it is important to evaluate the life cycle impacts associated with each scenario. The storage phase of food waste, encompassing its accumulation in kitchens, and storage in bins for collection and transportation, has been overlooked as a source of greenhouse gases in previous studies. This investigation aimed to identify the greenhouse gases emitted during the initial five-day period of low-oxygen storage. An open dynamic …


Investigating The Effectiveness Of Microplastic Removal And The Relationship Between Microplastics, Bacterial Communities, And Nitrogen Cycling In The Natural Treatment Systems At The Arcata Wastewater Treatment Facility, Fiona Connor Jan 2023

Investigating The Effectiveness Of Microplastic Removal And The Relationship Between Microplastics, Bacterial Communities, And Nitrogen Cycling In The Natural Treatment Systems At The Arcata Wastewater Treatment Facility, Fiona Connor

Cal Poly Humboldt theses and projects

This thesis addressed two challenging aspects of microplastic research, including the quantification of microplastics in organic-rich water, and the investigation of how microplastics impact microorganisms and related nutrient-cycling. Two projects were designed and executed to address specific research objectives relating to these broad topics. The first objective was to develop and implement methods to determine the effectiveness of microplastic removal across the natural treatment systems at the Arcata Wastewater Treatment Facility (AWTF). The second objective was to observe how microplastics found in domestic wastewater impact the bacterial communities and nitrogen cycling in freshwater treatment wetland sediment through a bench-scale experiment, …


Concentrated Wastewater Treatment Using A Ferric Iron-Dosed Anaerobic Upflow Sludge Blanket Reactor For Recovery Of Phosphorus And Ammonium, Carley E. Shingleton Jan 2023

Concentrated Wastewater Treatment Using A Ferric Iron-Dosed Anaerobic Upflow Sludge Blanket Reactor For Recovery Of Phosphorus And Ammonium, Carley E. Shingleton

Graduate Theses, Dissertations, and Problem Reports

This study evaluated the performance of a novel, ferric iron-dosed anaerobic bioreactor to recover two separate nutrient products from concentrated wastewaters -- phosphorus as vivianite (Fe3(PO4)2×8H2O) and an ammonium-containing effluent with low organics. A bench-scale upflow anaerobic sludge blanket (UASB) reactor fed with a synthetic wastewater (458.0 mg/L total organic carbon, 282.7 mg/L ammonium, and 84.4 mg/L phosphate) was dosed with a ferric chloride solution at an org. C/Fe3+ molar ratio ~17.5 to facilitate organic carbon oxidation coupled to iron reduction. The reactor design allowed natural settling of vivianite to its …


Bioaccumulation Of Polychlorinated Biphenyl Compounds And Mercury In A Mining Impacted Aquatic Ecosystem, Michelle Bollini Jan 2023

Bioaccumulation Of Polychlorinated Biphenyl Compounds And Mercury In A Mining Impacted Aquatic Ecosystem, Michelle Bollini

Dissertations, Master's Theses and Master's Reports

The Keweenaw area continues to be influenced by the century of copper mining that ended nearly 50 years ago. This project is focused on Torch Lake, an aquatic ecosystem that has been heavily impacted by mining waste disposal. The watershed has been impaired by mine discharge and tailings, smelter and smokestack plumes, and poor waste disposal practices. The lake is listed as a Great Lakes Area of Concern with beneficial use impairments of restrictions on fish consumption and a degraded benthic community. Polychlorinated biphenyl compounds (PCBs) and methylmercury (MeHg) are persistent, bioaccumulative, and toxic substances (PBTs). These contaminants pose threats …


Eutrophication On Twin Lakes: Applying A Phosphorus Budget And Shoreline Surveys To Assess Current & Historical Lake Health, Tyler K. Lemahieu Jan 2023

Eutrophication On Twin Lakes: Applying A Phosphorus Budget And Shoreline Surveys To Assess Current & Historical Lake Health, Tyler K. Lemahieu

Dissertations, Master's Theses and Master's Reports

The Twin Lakes system, a series of lakes including Lake Gerald and Lake Roland, was historically categorized as oligotrophic but is now mesotrophic having experienced cultural eutrophication. A mass balance phosphorus budget model was constructed for the Twin Lakes system in Houghton, Co. Michigan for the 2022 stratified season. Additional spatial insights were gleaned through a survey of shoreline water quality, which corroborated the model results. The lakes currently experience substantial depletion of hypolimnetic dissolved oxygen during late summer stratification. A budget reveals approximately 22% (6.6 kg) of the current 29.5 kg total phosphorus inputs to Twin Lakes (during the …


Winter Dynamics Of Storm Water Management Ponds And Winter Tolerance In Three Aquatic Plant Species, Patrick Strzalkowski Jan 2023

Winter Dynamics Of Storm Water Management Ponds And Winter Tolerance In Three Aquatic Plant Species, Patrick Strzalkowski

Theses and Dissertations (Comprehensive)

The vast majority of the research into the performance of stormwater management ponds (SWMPs) has been performed in warm regions or during the warmer seasons in temperate regions. It is presumed that SWMPs are inactive in the winter as any potential stormwater is trapped in snow and ice. The main goal of this thesis was to test this presumption and to study the dynamics and performance of three SWMPs during the winter. Remote water level loggers were installed into the three SWMPs and daily grab samples from the influents and effluents were taken and analyzed for total phosphorus (TP), chloride, …