Civil and Environmental Engineering Commons^™

Bioactive Surface Modification Of Metal Oxides Via Catechol-Bearing Modular Peptides: Multivalent-Binding, Surface Retention, And Peptide Bioactivity, Wen Tang, Gina Policastro, Geng Hua, Kai Guo, Chrys Wesdemiotis, Gary Doll, Matthew Becker

Gary L. Doll

A series of multivalent dendrons containing a bioactive osteogenic growth peptide (OGP) domain and surface-binding catechol domains were obtained through solid phase synthesis, and their binding affinity to hydroxyapatite, TiO2, ZrO2, CeO2, Fe3O4 and gold was characterized using a quartz crystal microbalance with dissipation (QCM-d). Using the distinct difference in binding affinity of the bioconjugate to the metal oxides, TiO2-coated glass slides were selectively patterned with bioactive peptides. Cell culture studies demonstrated the bioavailability of the OGP and that OGP remained on the surface for at least 2 weeks under in vitro cell culture conditions. Bone sialoprotein (BSP) and osteocalcein …