Chemical Engineering Commons^™

Studies On Improved Integrated Membrane-Based Chromatographic Process For Bioseparation, Yanke Xu

Dissertations

To improve protein separation and purification directly from a fermentation broth, a novel membrane filtration-cum-chromatography device configuration having a relatively impermeable coated zone near the hollow fiber module outlet has been developed. The integrated membrane filtration-cum-chromatography unit packed with chromatographic beads on the shell side of the hollow fiber unit enjoys the advantages of both membrane filtration and chromatography; it allows one to load the chromatographic media directly from the fermentation broth or lysate and separate the adsorbed proteins through the subsequent elution step in a cyclic process.

Interfacial polymerization was carried out to coat the bottom section of the …