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Articles 1 - 16 of 16
Full-Text Articles in Chemical Engineering
Ranque–Hilsch Vortex Tube Thermocycler For Fast Dna Amplification And Real-Time Optical Detection, Ryan J. Ebmeier, Scott E. Whitney, Amitabha Sarkar, Michael Nelson, Nisha Padhye, George Gogos, Hendrik J. Viljoen
Ranque–Hilsch Vortex Tube Thermocycler For Fast Dna Amplification And Real-Time Optical Detection, Ryan J. Ebmeier, Scott E. Whitney, Amitabha Sarkar, Michael Nelson, Nisha Padhye, George Gogos, Hendrik J. Viljoen
Papers in Biochemical Engineering
An innovative polymerase chain reaction (PCR) thermocycler capable of performing real-time optical detection is described below. This device utilizes the Ranque–Hilsch vortex tube in a system to efficiently and rapidly cycle three 20 mL samples between the denaturation, annealing, and elon-gation temperatures. The reaction progress is displayed real-time by measuring the size of a fluo-rescent signal emitted by SYBR green/double-stranded DNA complexes. This device can produce significant reaction yields with very small amounts of initial DNA, for example, it can amplify 0.25 fg (,5 copies) of a 96 bp bacteriophage l-DNA fragment 2.731011-fold by performing 45 cycles in less than …
Thermodynamic Analysis Of Separation Systems, Yaşar Demirel
Thermodynamic Analysis Of Separation Systems, Yaşar Demirel
Papers in Thermal Mechanics
Separation systems mainly involve interfacial mass and heat transfer as well as mixing. Distillation is a major separation system by means of heat supplied from a higher temperature level at the reboiler and rejected in the condenser at a lower temperature level. Therefore, it resembles a heat engine producing a separation work with a rather low efficiency. Lost work (energy) in separation systems is due to irreversible processes of heat, mass transfer, and mixing, and is directly related to entropy production according to the Gouy-Stodola principle. In many separation systems of absorption, desorption, extraction, and membrane separation, the major irreversibility …
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Ethylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Jayanta Sinha, Bradley A. Plantz, Mehmet Inan, Michael Meagher
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Ethylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Jayanta Sinha, Bradley A. Plantz, Mehmet Inan, Michael Meagher
Papers in Biotechnology
It was observed that during fermentative production of recombinant ovine interferon-H (r-oIFN-H ) in Pichia pastoris, a secreted recombinant protein, the protein was degraded increasingly after 48 h of induction and the rate of degradation increased towards the end of fermentation at 72 h, when the fermentation was stopped. Proteases, whose primary source was the vacuoles, was found in in-creasing levels in the cytoplasm and in the fermentation broth after 48 h of induction and reached maximal values when the batch was completed at 72 h. Protease levels at various cell fractions as well as in the culture supernatant were …
Stable Zeolite/Cellulose Composite Materials And Method Of Preparation, Gustavo F. Larsen
Stable Zeolite/Cellulose Composite Materials And Method Of Preparation, Gustavo F. Larsen
Department of Chemical and Biomolecular Engineering: Patents
Stable cellulosic fiber material for use in forming zeolite/cellulose composites is prepared by suspending loose cellulose fibers in an aqueous solution of sodium hydroxide, potassium hydroxide or sodium silicate, stirring the resulting suspension until it reaches a macroscopically homogenous appearance, heating the resulting mixture at a temperature of 323-423 K until only dry solids remain, contacting the resulting mixture with excess distilled water to remove physically adsorbed or trapped sodium hydroxide, potassium hydroxide or sodium silicate from the fibers, and heating the resulting fiber material at 323-423 K to dry the fiber material. Stable zeolite/cellulose composite material characterized in that …
Exergy Use In Bioenergetics, Yaşar Demirel
Exergy Use In Bioenergetics, Yaşar Demirel
Papers in Analytical Chemistry
Every developed and adapted biological system extracts useful energy from outside, converts, stores it, and uses for muscular contraction, substrate transport, protein synthesis, and other energy utilizing processes. This energy management in a living cell is called the bioenergetics, and the useful energy is the exergy, which is destroyed in every irreversible process because of the entropy production. The converted exergy is the adenosine triphosphate (ATP) produced through the oxidative phosphorylation coupled to respiration in which the exergy originates from oxidation of reducing equivalents of nutrients. A living cell uses the ATP for all the energy demanding activities; it has …
Haemophilic Factors Produced By Transgenic Livestock: Abundance That Can Enable Alternative Therapies Worldwide, Kevin E. Van Cott, Paul E. Monahan, Timothy C. Nichols, William H. Velander
Haemophilic Factors Produced By Transgenic Livestock: Abundance That Can Enable Alternative Therapies Worldwide, Kevin E. Van Cott, Paul E. Monahan, Timothy C. Nichols, William H. Velander
Papers in Biotechnology
Haemophilia replacement factors, both plasma-derived and recombinant, are in relatively short supply and are high-cost products. This has stymied the study and development of alternative methods of administration of haemophilia therapy even in the most economically advanced countries, owing to the large amounts of material needed because bioabsorption and bioavailability of haemophilic factors can be less than 10% when using non-intravenous routes of delivery. There is therefore a need to increase access to therapy worldwide by decreasing the cost and increasing the abundance so that therapy can be achieved through simplified, alternative delivery methods. Transgenic livestock have been used to …
Production And Purification Of A Chimeric Monoclonal Antibody Against Botulinum Neurotoxin Serotype A , Mark C. Mowry, Michael Meagher Lead Investigator, Leonard Smith, Anuradha Subramanian
Production And Purification Of A Chimeric Monoclonal Antibody Against Botulinum Neurotoxin Serotype A , Mark C. Mowry, Michael Meagher Lead Investigator, Leonard Smith, Anuradha Subramanian
Papers in Biotechnology
Production of recombinant antibodies against botulinum neurotoxin is necessary for the development of a post-exposure treatment. CHO-DG44 cells were transfected with a plasmid encoding the light and heavy chains of a chimeric monoclonal antibody (S25) against botulism neurotoxin serotype A. Stable cell lines were obtained by dilution cloning and clones were shown to produce nearly equivalent levels of light and heavy chain antibody by an enzyme-linked immunosorbent assay (ELISA). In suspension culture, cells produced 35 μg/ml of chimeric antibody after 6 days, corresponding to a specific antibody productivity of 3.1 pg/cell/day. A method for the harvest and recovery of an …
Chemical/Mechanical Analyses Of Anhydride-Cured Thermosetting Epoxys: Dgeba/Nma/Bdma, Wei Chian, Delmar C. Timm
Chemical/Mechanical Analyses Of Anhydride-Cured Thermosetting Epoxys: Dgeba/Nma/Bdma, Wei Chian, Delmar C. Timm
Papers in Molecular Chemistry
The chemical state of cure in a thermosetting resin was used to predict the resin’s equilibrium modulus. High performance liquid chromatography analyses of the sol fraction yielded molar dynamics for monomeric, oligomeric, and polymeric molecules. Their population density distributions were compared with theoretical predictions based on a chain-growth polymerization mechanism. The resulting chemical estimates of the state of cure were integrated into calculations yielding concentrations of network structures within the gel that contribute to the density of elastically active strands and junctions. The theory of rubber elasticity was then used to predict the equilibrium modulus. Measurements incorporated dynamic mechanical analysis. …
Kinetic Reaction Analysis Of An Anhydride-Cured Thermoplastic Ep-Oxy:Pge/Nma/Bdma, Wei Chian, Delmar C. Timm
Kinetic Reaction Analysis Of An Anhydride-Cured Thermoplastic Ep-Oxy:Pge/Nma/Bdma, Wei Chian, Delmar C. Timm
Papers in Molecular Chemistry
A comprehensive reaction analysis of a linear epoxy resin cured with an anhydride was performed to evaluate the reaction rate expressions. Monomers included phenyl glycidyl ether and methyl- 5-norbornene-2,3-dicarboxylic anhydride or nadic methyl anhydride; the catalyst was N,N-dimethylbenzylamine; the initiator was n-propanol. Emphasis was initially placed on the molar dynamics of monomeric and oligomeric molecules. Molecular fractionations were achieved using reversed phase, high performance liquid chromatography. Chemical reaction rate constants were examined as a function of degree of polym-erization. For the chain-initiated polymerization, the initiation rate constant was observed to be approximately 3 times greater than the propagation constant associated …
Final Report: Engineering Design Of Stable Immobilized Enzymes For The Hydrolysis And Transesterification Of Triglycerides, Dr.Hossein Noureddini, Gustavo F. Larsen
Final Report: Engineering Design Of Stable Immobilized Enzymes For The Hydrolysis And Transesterification Of Triglycerides, Dr.Hossein Noureddini, Gustavo F. Larsen
Department of Chemical and Biomolecular Engineering: Funded Proposals
Enzyme Immobilization. Work will continue in the area of enzymatic transesterification reaction (biodiesel). Both methyl and ethyl esters will be used in this study. Unlike the chemical reaction where methanol has a clear advantage over ethanol, ethanol can be used as easily as methanol in the enzymatic reaction.
Sol/Gel Structure Modification. Work will concentrate on the effect of the vacuum procedure on pore size and distribution for the transesterification reaction. Additives such as glucose have been very effective in the hydrolysis reaction and will be explored further in the transesterification reaction.
Characterization. The developed material will be characterized for the …
Optimization Of Cell Density And Dilution Rate In Pichia Pastoris Continuous Fermentations For Production Of Recombinant Proteins, Mehmet Inan, Michael M. Meagher, Wenhui Zhang, Chih Ping Liu
Optimization Of Cell Density And Dilution Rate In Pichia Pastoris Continuous Fermentations For Production Of Recombinant Proteins, Mehmet Inan, Michael M. Meagher, Wenhui Zhang, Chih Ping Liu
Papers in Biomolecular Engineering
This paper provides an approach for optimizing the cell density (Xc) and dilution rate (D) in a chemostat for a Pichia pastoris continuous fermentation for the extracellular production of a recombinant protein, interferon τ (INF-τ). The objective was to maximize the volumetric productivity (Q, mg INF-τ I-1 h-1), which was accomplished using response surface methodology (RSM) to model the response of Q as a function of Xc and D within the ranges 150 ≤ Xc ≤ 450 g cells (wet weight) l-1 and 0.1 μm ≤D ≤ 0.9 μm (μm =0.0678 h-1, the maximum specific growth rate obtained from a …
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Methylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Mehmet Inan, Michael M. Meagher, Bradley A. Plantz, Jayanta Sinha
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Methylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Mehmet Inan, Michael M. Meagher, Bradley A. Plantz, Jayanta Sinha
Papers in Biochemical Engineering
It was observed that during fermentative production of recombinant ovine interferon-H (r-oIFN-H ) in Pichia pastoris, a secreted recombinant protein, the protein was degraded increasingly after 48 h of induction and the rate of degradation increased towards the end of fermentation at 72 h, when the fermentation was stopped. Proteases, whose primary source was the vacuoles, was found in increasing levels in the cytoplasm and in the fermentation broth after 48 h of induction and reached maximal values when the batch was completed at 72 h. Protease levels at various cell fractions as well as in the culture supernatant were …
Bio-Chip, Photoluminescent Methods For Identifying Biological Material, And Apparatuses For Use With Such Methods And Bio-Chips, Ravi F. Saraf
Bio-Chip, Photoluminescent Methods For Identifying Biological Material, And Apparatuses For Use With Such Methods And Bio-Chips, Ravi F. Saraf
Department of Chemical and Biomolecular Engineering: Patents
A method detects binding of molecules, advantageously without tagging molecules in the sample. A sensor is used in which is included a single stranded nucleic acid sequence and a photoluminescent material in respective layers. After the sensor is exposed to a biological sample for sufficient time for its single stranded nucleic acid sequence to bind to a material of interest, photoluminescence from the sensor can be measured. An apparatus for tagging-free detection of binding of molecules also is provided. Methods of making tagging-free sensors are provided. Also, tagging-free methods to detect binding of antigens and related devices are disclosed.
High-Resolution Touch Sensor Could Be Boon To Cancer Surgeons, Others
High-Resolution Touch Sensor Could Be Boon To Cancer Surgeons, Others
Department of Chemical and Biomolecular Engineering: News Releases
Lincoln, Neb., June 8, 2006 -- One of the trickiest decisions facing a cancer surgeon today is where to stop cutting. The surgeon doesn't want to stop too soon and leave cancer cells in the patient's body, but he or she also doesn't want to take too many cells and do unnecessary damage to organs. That decision could soon be made much easier, though, thanks to a high-resolution touch sensor developed by chemical engineers at the University of Nebraska-Lincoln that may allow surgeons to tell at the level of a single layer of cells whether or not they have excised …
Teaching Coupled Transport And Rate Processes, Yaşar Demirel
Teaching Coupled Transport And Rate Processes, Yaşar Demirel
Department of Chemical and Biomolecular Engineering: News Releases
Coupling refers to a flux occurring without its primary thermodynamic driving force; for example, mass flux without a concentration gradient called the thermal diffusion is a well-known coupled process. Coupling also refers to a flux occurring in a direction opposite to the direction imposed by its driving force; for example, a mass flux can occur from a low to a high concentration region and is called the active transport or uphill transport, such as potassium and sodium pumps coupled to chemical energy released by the hydrolysis of adenosine triphosphate (ATP) in biological systems. Although the coupled processes seem to be …
Teaching Engineering Courses With Workbooks, Yaşar Demirel
Teaching Engineering Courses With Workbooks, Yaşar Demirel
Department of Chemical and Biomolecular Engineering: News Releases
Society expects that a modem college education will turn out students who are analytical. intellectually curious, culturally aware, employable, and capable of leadership. Some important skills needed for all degree programs are problem solving, communication (written and oral), team or group work, learning and information processing, and technology. Instructors feel rewarded and satisfied when they sense that they have made a difference in the life of a student.