Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Chemical Engineering
An Approach To Sequence Dna Without Tagging, Sanjun Niu, Ravi F. Saraf
An Approach To Sequence Dna Without Tagging, Sanjun Niu, Ravi F. Saraf
Papers in Biotechnology
Microarray technology is playing an increasingly important role in biology and medicine and its application to genomics for gene expression analysis has already reached the market with a variety of commercially available instruments. In these combinatorial analysis methods, known probe single-strand DNA (ssDNA) ‘primers’ are attached in clusters of typically 100 μm × 100 μm pixels. Each pixel of the array has a slightly different sequence. On exposure to ‘unknown’ target ssDNA, the pixels with the right complementary probe ssDNA sequence convert to double-stranded DNA (dsDNA) by a hybridization reaction. To transduct the conversion of the pixel to dsDNA, the …
Expression Of Active Human Factor Ix In Mammary Tissue And Of Milk Non Human Transgenic Mammals , William H. Velander, William N. Drohan
Expression Of Active Human Factor Ix In Mammary Tissue And Of Milk Non Human Transgenic Mammals , William H. Velander, William N. Drohan
Papers in Biotechnology
Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5'-untranslated and 3'-untranslated region, which is …