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Full-Text Articles in Chemical Engineering
Modeling Pichia Pastoris Growth On Methanol And Optimizing The Production Of A Recombinant Protein, The Heavy-Chain Fragment C Of Botulinum Neurotoxin, Serotype A, Wenhui Zhang, Mark A. Bevins, Bradley A. Plantz, Leonard A. Smith, Michael M. Meagher
Modeling Pichia Pastoris Growth On Methanol And Optimizing The Production Of A Recombinant Protein, The Heavy-Chain Fragment C Of Botulinum Neurotoxin, Serotype A, Wenhui Zhang, Mark A. Bevins, Bradley A. Plantz, Leonard A. Smith, Michael M. Meagher
Papers in Biotechnology
An unstructured growth model for the recombinant methylotrophic yeast P. pastoris Mut+ expressing the heavy-chain fragment C of botulinum neurotoxin serotype A [BoNT/A(Hc)], was successfully established in quasi-steady state fed-batch fermentations with varying cell densities. The model describes the relationships between specific growth rate and methanol concentration, and the relationships between specific methanol and ammonium consump-tion rates and specific growth rate under methanol-limited growth conditions. The maximum specific growth rate (μ) determined from the model was 0.08 h−1 at a methanol concentration of 3.65 g/L, while the actual maximum μ was 0.0709 h−1. The maximum specific methanol consumption rate was …
Immunoaffinity Chomatography, Anuradha Subramanian
Immunoaffinity Chomatography, Anuradha Subramanian
Papers in Biotechnology
Recent devclopments in recombinant DNA technology have enabled the synthesis of valuable therapeutic proteins in bacterial cells as well as in novel eucaryotic expression systems. However, the purification of proteins of interest from either the conventional sources, cell culture, or novel routes in a highly purified form necessitates the development of separation tcchniques capable of recovering proteins from these feed streams in a highly purified form (1,2). Purification of therapeutic proteins from biological sources is usually complicated by the presence of endogenous proteins (2). Purification methodologies based on ion exchange or adsorption serve as excellent prepurification steps, but they fail …
Purification Of Immunoglobulins From Serum Using Thiophilic Cellulose Beads, Anuradha Subramanian
Purification Of Immunoglobulins From Serum Using Thiophilic Cellulose Beads, Anuradha Subramanian
Papers in Biotechnology
This study evaluates the chromatographic performance of a support obtained by the reaction of mercaptoethanol with cellulose beads activated with divinyl sulfone. Cellulose beads 500-800 microns (pm) in diameter and with a solids content of 3.5% were selected for this study. A two-step sequence of permeation and reaction was used to install thiophilic sites throughout the cross section of the bead. The distribution of thiophilic sites was visualized by immobilizing fluorescent antibodies. Human and porcine serum proteins were separated on the thiophilic support at different linear velocities. Thiophilic cellulose beads were observed to bind human and porcine immunoglobulins (IgG) selectively …
Ranking The Factors Impacting Lmmunosorbent Performance, Anuradha Subramanian, William H. Velander
Ranking The Factors Impacting Lmmunosorbent Performance, Anuradha Subramanian, William H. Velander
Papers in Biotechnology
This work addresses the relative impact of the phenomena of orientation, multipoint attachment, and local density of the immobilized antibody 011 immunosorbent perfornnance. The masking of the antigen binding domains of monoclonal antibodies (Mab) by Fab Masking Antigens (FMAs) was used as a tool to determine the impact of orientation on the perforn~anceo f Emphaze, Affiprep'M, and Cellulose bead-based immunosorbents. A two-step antibody immobilization inethod involving permeation of the beaded matrix followed by a fast coupling reaction was used to study the effect of local density of the Mnh on immunosorbent efficiency. Lysyl groups on Mabs were covalently modified to …