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- Antibody orientation; immunosorbent performance (1)
- Chromatography (1)
- Computational fluid dynamics (CFD) (1)
- Discrete cell model (DCM) (1)
- Fed-batch growth (1)
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- Fermentation optimization (1)
- Flow distribution (1)
- Genetics (1)
- Growth modeling (1)
- Hybridization (1)
- Immunoaffinity (1)
- Interfaces (1)
- Internal structure nonuniformities (1)
- Methylotrophic yeast (1)
- Microbial processes (1)
- Minimization of energy dissipation rate (1)
- Modulating functions (1)
- Molecules (1)
- Monolayers (1)
- Packed beds (1)
- Parameter estimation (1)
- Pearson-Lee method (1)
- Pichia pastoris (1)
- Pichia pastoris; fed-batch; growth modeling;optimization; fermentation; botulinum neurotoxin (1)
- Purification of immunoglobulins (1)
- Recombinant protein expression (1)
- Thiophilic Cellulose Beads (1)
- Time-varying systems (1)
Articles 1 - 14 of 14
Full-Text Articles in Chemical Engineering
Time-Varying System Identification Using Modulating Functions And Spline Models With Application To Bio-Processes, Sridhar Ungarala, Tomas B. Co
Time-Varying System Identification Using Modulating Functions And Spline Models With Application To Bio-Processes, Sridhar Ungarala, Tomas B. Co
Chemical & Biomedical Engineering Faculty Publications
Time dependent parameters are frequently encountered in many real processes which need to be monitored for process modeling, control and supervision purposes. Modulating functions methods are especially suitable for this task because they use the original continuous-time differential equations and avoid differentiation of noisy signals. Among the many versions of the method available, Pearson–Lee method offers a computationally efficient alternative. In this paper, Pearson–Lee method is generalized for non-stationary continuous-time systems and the on-line version is developed. The time dependent parameters are modeled as polynomial splines inside a moving data window and recursion formulae using shifting properties of …
Novel Processing Of Hydroxyapatite-Zirconia Composites Using Nano-Sized Particles, Mark R. Towler, I. R. Gibson, S. M. Best
Novel Processing Of Hydroxyapatite-Zirconia Composites Using Nano-Sized Particles, Mark R. Towler, I. R. Gibson, S. M. Best
Chemical and Biochemical Engineering Faculty Research & Creative Works
A method to produce composites of hydroxyapatite (HA) with ZrO2 (Y-TZP) additions between 0 and 10 wt % at a low sinter temperature of 750 °C is presented. The method limits the amount of reaction between the HA and ZrO2. A ZrO2 loading level that would improve strength while limiting the decomposition of the HA is studied.
Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh
Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh
Faculty Works
The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …
Modeling Pichia Pastoris Growth On Methanol And Optimizing The Production Of A Recombinant Protein, The Heavy-Chain Fragment C Of Botulinum Neurotoxin, Serotype A, Wenhui Zhang, Mark A. Bevins, Bradley A. Plantz, Leonard A. Smith, Michael M. Meagher
Modeling Pichia Pastoris Growth On Methanol And Optimizing The Production Of A Recombinant Protein, The Heavy-Chain Fragment C Of Botulinum Neurotoxin, Serotype A, Wenhui Zhang, Mark A. Bevins, Bradley A. Plantz, Leonard A. Smith, Michael M. Meagher
Papers in Biotechnology
An unstructured growth model for the recombinant methylotrophic yeast P. pastoris Mut+ expressing the heavy-chain fragment C of botulinum neurotoxin serotype A [BoNT/A(Hc)], was successfully established in quasi-steady state fed-batch fermentations with varying cell densities. The model describes the relationships between specific growth rate and methanol concentration, and the relationships between specific methanol and ammonium consump-tion rates and specific growth rate under methanol-limited growth conditions. The maximum specific growth rate (μ) determined from the model was 0.08 h−1 at a methanol concentration of 3.65 g/L, while the actual maximum μ was 0.0709 h−1. The maximum specific methanol consumption rate was …
Fermentation Strategies For Recombinant Protein Expression In The Methylotrophic Yeast Pichia Pastoris, Wenhui Zhang, Mehmet Inan, Michael M. Meagher
Fermentation Strategies For Recombinant Protein Expression In The Methylotrophic Yeast Pichia Pastoris, Wenhui Zhang, Mehmet Inan, Michael M. Meagher
Papers in Biochemical Engineering
Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.
Single Phase Flow Modeling In Packed Beds: Discrete Cell Approach Revisited, Y. Jiang, M. R. Khadilkar, M. (Muthanna) H. Al-Dahhan, M. P. Dudukovic
Single Phase Flow Modeling In Packed Beds: Discrete Cell Approach Revisited, Y. Jiang, M. R. Khadilkar, M. (Muthanna) H. Al-Dahhan, M. P. Dudukovic
Chemical and Biochemical Engineering Faculty Research & Creative Works
A Discrete Cell Model (DCM), based on the Minimization of the Total Rate of Energy Dissipation, is Applied to Compute the Fluid Velocity Field in Two-Dimensional Packed Beds. the Analysis of the Individual Terms of the Energy Dissipation Rate Equation is Also Presented. the Results Obtained by DCM Are Validated Both by Comparing Them with the Solutions of Ensemble-Averaged Momentum and Mass Conservation Equations (CFDLIB Code) and by Available Experimental Results. the Differences between DCM and CFD Simulations Were Found to Be Confined to within a 10% Band over a Wide Range of Reynolds Numbers (Re' = 5-171). Thus, a …
Immunoaffinity Chomatography, Anuradha Subramanian
Immunoaffinity Chomatography, Anuradha Subramanian
Papers in Biotechnology
Recent devclopments in recombinant DNA technology have enabled the synthesis of valuable therapeutic proteins in bacterial cells as well as in novel eucaryotic expression systems. However, the purification of proteins of interest from either the conventional sources, cell culture, or novel routes in a highly purified form necessitates the development of separation tcchniques capable of recovering proteins from these feed streams in a highly purified form (1,2). Purification of therapeutic proteins from biological sources is usually complicated by the presence of endogenous proteins (2). Purification methodologies based on ion exchange or adsorption serve as excellent prepurification steps, but they fail …
Effects Of Adding Sodium And Fluoride Ions To Glass Ionomer On Its Interactions With Sodium Fluoride Solution, R. W. Billington, P. C. Hadley, Mark R. Towler, G. J. Pearson, J. A. Williams
Effects Of Adding Sodium And Fluoride Ions To Glass Ionomer On Its Interactions With Sodium Fluoride Solution, R. W. Billington, P. C. Hadley, Mark R. Towler, G. J. Pearson, J. A. Williams
Chemical and Biochemical Engineering Faculty Research & Creative Works
This investigates the effects of the addition of Na and F ions to a glass ionomer cement in which those ions are not inherently present on its interactions with dilute (0.2%) NaF solution. Both the effect of the solution on the cement's surface morphology and the effect of the cement on the solution in terms of take up of Na+ and F- and of change in pH are to be investigated. These results are to be compared to previous results obtained with glasses which contained both, one, or neither of the ions as components of their glasses. NaF …
Purification Of Immunoglobulins From Serum Using Thiophilic Cellulose Beads, Anuradha Subramanian
Purification Of Immunoglobulins From Serum Using Thiophilic Cellulose Beads, Anuradha Subramanian
Papers in Biotechnology
This study evaluates the chromatographic performance of a support obtained by the reaction of mercaptoethanol with cellulose beads activated with divinyl sulfone. Cellulose beads 500-800 microns (pm) in diameter and with a solids content of 3.5% were selected for this study. A two-step sequence of permeation and reaction was used to install thiophilic sites throughout the cross section of the bead. The distribution of thiophilic sites was visualized by immobilizing fluorescent antibodies. Human and porcine serum proteins were separated on the thiophilic support at different linear velocities. Thiophilic cellulose beads were observed to bind human and porcine immunoglobulins (IgG) selectively …
The Influence Of Poly(Acrylic Acid) Molar Mass On The Properties Of Polyalkenoate Cements Formed From Zinc Oxide/Apatite Mixtures, Sinead Kenny, Robert G. Hill, Mark R. Towler
The Influence Of Poly(Acrylic Acid) Molar Mass On The Properties Of Polyalkenoate Cements Formed From Zinc Oxide/Apatite Mixtures, Sinead Kenny, Robert G. Hill, Mark R. Towler
Chemical and Biochemical Engineering Faculty Research & Creative Works
The influence of poly (acrylic acid) molar mass was investigated on cements formed from zinc oxide-apatite mixtures at three aging times: one, seven and 28 days. Cements based on both hydroxyapatite and fluorapatite were investigated. The compressive strength, un-notched fracture strength and fracture toughness increased markedly with poly (acrylic acid) molar mass. The fracture toughness and un-notched fracture strength increased with aging time for the two highest molar mass cements but decreased with time for the two lowest molar mass cements. The greater chain entanglement density present in the higher molar mass cements is thought to contribute significantly to the …
Discriminating Trickle-Flow Hydrodynamic Models: Some Recommendations, F. Larachi, I. Iliuta, M. (Muthanna) H. Al-Dahhan, M. P. Dudukovic
Discriminating Trickle-Flow Hydrodynamic Models: Some Recommendations, F. Larachi, I. Iliuta, M. (Muthanna) H. Al-Dahhan, M. P. Dudukovic
Chemical and Biochemical Engineering Faculty Research & Creative Works
The Forecasting Ability of Five One-Dimensional (1-D) Two-Fluid Phenomenological Models for Liquid Holdup and Two-Phase Pressure Drop in Trickle-Flow Reactors Was Evaluated using the Most Comprehensive Trickle-Flow Regime Database. All of These Models, Namely, the Permeability Model, the Slit Model, the Extended Slit Model, the 1-D CFD Model, and the Double-Slit Model Can Be Used to Predict Liquid Holdup. among Them, the Permeability and the Slit Models, Because of a Much Simpler Structure, Are Recommended. the Extended Slit Model based on Iliuta Et Al. (Ind. Eng. Chem. Res. 1998, 37, 4542) Shear and Slip Constitutive Relationships Can Be Employed for …
Ranking The Factors Impacting Lmmunosorbent Performance, Anuradha Subramanian, William H. Velander
Ranking The Factors Impacting Lmmunosorbent Performance, Anuradha Subramanian, William H. Velander
Papers in Biotechnology
This work addresses the relative impact of the phenomena of orientation, multipoint attachment, and local density of the immobilized antibody 011 immunosorbent perfornnance. The masking of the antigen binding domains of monoclonal antibodies (Mab) by Fab Masking Antigens (FMAs) was used as a tool to determine the impact of orientation on the perforn~anceo f Emphaze, Affiprep'M, and Cellulose bead-based immunosorbents. A two-step antibody immobilization inethod involving permeation of the beaded matrix followed by a fast coupling reaction was used to study the effect of local density of the Mnh on immunosorbent efficiency. Lysyl groups on Mabs were covalently modified to …
Drawbacks Of The Dissolution Method For Measurement Of The Liquid-Solid Mass-Transfer Coefficients In Two-Phase Flow Packed-Bed Reactors Operated At Low And High Pressures, Muthanna H. Al-Dahhan, Wes Highfill, Boon Tee Ong
Drawbacks Of The Dissolution Method For Measurement Of The Liquid-Solid Mass-Transfer Coefficients In Two-Phase Flow Packed-Bed Reactors Operated At Low And High Pressures, Muthanna H. Al-Dahhan, Wes Highfill, Boon Tee Ong
Chemical and Biochemical Engineering Faculty Research & Creative Works
The Dissolution Method Was Used Predominantly at Atmospheric Conditions to Measure the Liquid-Solid Mass-Transfer Coefficient in Two-Phase Flow Packed-Bed Reactors. in an Attempt to Investigate the Effect of Reactor Pressure and Gas Flow Rate on the Liquid-Solid Mass-Transfer Coefficient in a Trickle-Bed Reactor using the Dissolution Method, Difficulties Were Encountered in Implementing This Method at Low- to High-Pressure Operation. Noticeable Losses in the Dissolved Material Were Observed as a Result of Flashing/stripping from the Liquid Phase to the Atmosphere and the Effluent Gas Stream. This Leads to Misinterpretation of the Effect of Reactor Pressure and to Improper Conclusions. in This …
Double-Slit Model For Partially Wetted Trickle Flow Hydrodynamics, I. Iliuta, F. Larachi, M. (Muthanna) H. Al-Dahhan
Double-Slit Model For Partially Wetted Trickle Flow Hydrodynamics, I. Iliuta, F. Larachi, M. (Muthanna) H. Al-Dahhan
Chemical and Biochemical Engineering Faculty Research & Creative Works
A Double-Slit Model Developed Can Predict the Frictional Two-Phase Pressure Drop, External Liquid Holdup, Pellet-Scale External Wetting Efficiency, and Gas - Liquid Interfacial Area in Cocurrent Downflow Trickle-Bed Reactors Operated under Partially Wetted Conditions in the Trickle Flow Regime. the Model, an Extension of the Holub Et Al. (1992, 1993) Mechanistic Pore-Scale Phenomenological Approach, Was Designed to Mimic the Actual Bed Void by Two Inclined and Interconnected Slits: Wet and Dry Slit. the External Wetting Efficiency is Linked to Both the Pressure Drop and External Liquid Holdup. the Model Also Predicts Gas - Liquid Interfacial Areas in Partially Wetted Conditions. …