Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Biochemical and Biomolecular Engineering (31)
- Polymer Science (9)
- Physical Sciences and Mathematics (7)
- Biomedical Engineering and Bioengineering (4)
- Chemistry (4)
-
- Materials Science and Engineering (4)
- Transport Phenomena (4)
- Biomedical Devices and Instrumentation (3)
- Mechanical Engineering (3)
- Other Chemical Engineering (3)
- Analytical Chemistry (2)
- Electrical and Computer Engineering (2)
- Electromagnetics and Photonics (2)
- Environmental Sciences (2)
- Membrane Science (2)
- Oil, Gas, and Energy (2)
- Optics (2)
- Physical Chemistry (2)
- Physics (2)
- Semiconductor and Optical Materials (2)
- Aerospace Engineering (1)
- Earth Sciences (1)
- Engineering Education (1)
- Geology (1)
- Heat Transfer, Combustion (1)
- Nuclear Engineering (1)
- Other Materials Science and Engineering (1)
- Petroleum Engineering (1)
- Institution
-
- Missouri University of Science and Technology (21)
- University of Nebraska - Lincoln (21)
- Brigham Young University (17)
- The University of Akron (8)
- University of South Carolina (7)
-
- Cleveland State University (6)
- University of Nevada, Las Vegas (3)
- University of Tennessee, Knoxville (3)
- San Jose State University (2)
- University of Massachusetts Amherst (2)
- University of Rhode Island (2)
- The University of Maine (1)
- University of Dayton (1)
- University of Kentucky (1)
- University of New Haven (1)
- Keyword
-
- Biomass (4)
- Fuel (4)
- XPS (4)
- CARPT (3)
- Computed tomography (3)
-
- Flow distribution (3)
- Liquid saturation (3)
- Molecular dynamics method (3)
- Monolith (3)
- 07. Selected Conferences on Polymer Science (2)
- Adsorption (2)
- Agricultural residues (2)
- Biomass energy sources (2)
- Cavitation (2)
- Chlamydomonas reinhardtii (2)
- Drug delivery (2)
- Electrochemical electrodes (2)
- Electrochemistry · gold · monolayers nanostructures (2)
- Elemental analysis (2)
- Engineering (2)
- Filtration (2)
- Flow simulation (2)
- Gas Turbine (2)
- History of Polymer Science (2)
- Mammary gland expression (2)
- Nitric acid; Plutonium; Radioactive wastes – Purification; Separation (Technology); Speciation (Chemistry); Tributyl phosphate; Uranium (2)
- Nitrogen oxide (2)
- Organic compounds (2)
- Otto Vogl (2)
- Polymer Science Conferences (2)
- Publication
-
- Faculty Publications (26)
- Chemical and Biochemical Engineering Faculty Research & Creative Works (20)
- Papers in Biotechnology (9)
- College of Polymer Science and Polymer Engineering (8)
- Chemical & Biomedical Engineering Faculty Publications (6)
-
- Papers in Biochemical Engineering (5)
- Faculty Publications and Other Works -- Chemical and Biomolecular Engineering (3)
- Chemical Engineering Faculty Publications (2)
- Department of Chemical and Biomolecular Engineering: News Releases (2)
- Emeritus Faculty Author Gallery (2)
- Separations Campaign (TRP) (2)
- Chemical and Materials Engineering Faculty Patents (1)
- Department of Chemical and Biomolecular Engineering: Courses (1)
- Department of Chemical and Biomolecular Engineering: Funded Proposals (1)
- Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works (1)
- Mechanical Engineering Faculty Research (1)
- Mechanical and Aerospace Engineering Faculty Publications (1)
- Mechanical and Industrial Engineering Faculty Publications (1)
- Papers in Enzyme Kinetics (1)
- Papers in Fluid Mechanics (1)
- Papers in Thermal Mechanics (1)
- University of Maine Office of Research Administration: Grant Reports (1)
Articles 1 - 30 of 96
Full-Text Articles in Chemical Engineering
Analysis Of Grain Screenings By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Analysis Of Grain Screenings By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Faculty Publications
Determining the chemical structure and composition of biomass fuels using x-ray photoelectron spectroscopy XPS can provide fundamental knowledge of their structures that is useful in understanding and predicting their combustion behavior. Grain screenings are an example of an agricultural residue byproduct of food and feed production of potential interest for biomass combustion. The XPS spectra of grain screenings provide both its elemental composition and indications of its bonding. Traditional fuel analyses of this fuel are also provided. These include: ultimate analysis — the elemental composition of the overall fuel C, H, N, S, and O; chlorine analysis — reported here …
Analysis Of Sugar Beet Pulp By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Analysis Of Sugar Beet Pulp By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Faculty Publications
Determining the chemical structure and composition of biomass fuels using x-ray photoelectron spectroscopy XPS can provide fundamental knowledge of their structures that is useful in understanding and predicting their combustion behavior. Sugar beet pulp is an example of an agricultural residue byproduct of food and feed production of potential interest for biomass combustion. The XPS spectra of sugar beet pulp provide both its elemental composition and indications of its bonding. Traditional fuel analyses of this fuel are also provided. These include: ultimate analysis — the elemental composition of the overall fuel C, H, N, S, and O; chlorine analysis — …
Research Article| December 30 2005 Analysis Of Shea Nut Shells By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Research Article| December 30 2005 Analysis Of Shea Nut Shells By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Faculty Publications
Determining the chemical structure and composition of biomass fuels using x-ray photoelectron spectroscopy (XPS) can provide fundamental knowledge of their structures that is useful in understanding and predicting their combustion behavior. Shea nut shells are an example of an agricultural residue (byproduct of food and feed production) of potential interest for biomass combustion. The XPS spectra of shea nut shells provide both its elemental composition and indications of its bonding. Traditional fuel analyses of this fuel are also provided. These include: ultimate analysis — the elemental composition of the overall fuel (C, H, N, S, and O); chlorine analysis — …
Analysis Of Sunflower Shells By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Analysis Of Sunflower Shells By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Faculty Publications
Determining the chemical structure and composition of biomass fuels using x-ray photoelectron spectroscopy (XPS) can provide fundamental knowledge of their structures that is useful in understanding and predicting their combustion behavior. Sunflower shells are an example of an agricultural residue (byproduct of food and feed production) of potential interest for biomass combustion. The XPS spectra of sunflower shells provide both its elemental composition and indications of its bonding. Traditional fuel analyses of this fuel are also provided. These include: ultimate analysis — the elemental composition of the overall fuel (C, H, N, S, and O); chlorine analysis — reported here …
Analysis Of Sawdust By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Analysis Of Sawdust By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Faculty Publications
Determining the chemical structure and composition of biomass fuels using x-ray photoelectron spectroscopy XPS can provide fundamental knowledge of their structures that is useful in understanding and predicting their combustion behavior. Sawdust is an example of a forest product residue byproduct of paper and lumber production of potential interest for biomass combustion. The XPS spectra of sawdust provide both its elemental composition and indications of its bonding. Traditional fuel analyses of this fuel are also provided. These include: ultimate analysis — the elemental composition of the overall fuel C, H, N, S, and O; chlorine analysis — reported here as …
Analysis Of Straw By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Analysis Of Straw By X-Ray Photoelectron Spectroscopy, Guilin Jiang, Ghaleb A. Husseini, Larry Lin Baxter, Matthew R. Linford
Faculty Publications
Determining the chemical structure and composition of biomass fuels using x-ray photoelectron spectroscopy XPS can provide fundamental knowledge of their structures that is useful in understanding and predicting their combustion behavior. Straw is an example of an agricultural residue byproduct of food and feed production of potential interest for biomass combustion. The XPS spectra of straw provide both its elemental composition and indications of its bonding. Traditional fuel analyses of this fuel are also provided. These include: ultimate analysis — the elemental composition of the overall fuel C, H, N, S, and O; chlorine analysis — reported here as part …
Experimental Study Of The Solids Velocity Field In Gas-Solid Risers, Satish Bhusarapu, Muthanna H. Al-Dahhan, Milorad P. Duduković, Steven Trujillo, Timothy J. O'Hern
Experimental Study Of The Solids Velocity Field In Gas-Solid Risers, Satish Bhusarapu, Muthanna H. Al-Dahhan, Milorad P. Duduković, Steven Trujillo, Timothy J. O'Hern
Chemical and Biochemical Engineering Faculty Research & Creative Works
Solids Flow Dynamics in Gas-Solid Risers is Inherently Complex. Model Refinement through Experimental Validation Requires the Acquisition of Detailed Nonintrusive Measurements. in This Study, Noninvasive Computer-Automated Radioactive Particle Tracking (CARPT) is Employed to Visualize and Quantify in a Three-Dimensional Domain the Solids Dynamics and Mixing in Gas-Solid Risers. This Technique Has the Added Advantage that, along with the Derived Eulerian Solids Flow Field (Time-Average Velocity Map and Various Turbulence Parameters Such as the Reynolds Stresses, Turbulent Kinetic Energy), It Also Provides Directly the Lagrangian Description of the Solids Motion. the Solids Velocity Field Data Are Obtained in Two Different Risers …
54th Spsj Annual Meeting Yokohama, Japan, May 25-27, 2005, Jane C. Vogl, Hiroyuki Ohno
54th Spsj Annual Meeting Yokohama, Japan, May 25-27, 2005, Jane C. Vogl, Hiroyuki Ohno
Emeritus Faculty Author Gallery
No abstract provided.
Act/Sger: Atomic Layer Deposition Of Nitrides On Nano-Particles For Enhanced Energy Conversion To Combat Terrorism, William J. Desisto
Act/Sger: Atomic Layer Deposition Of Nitrides On Nano-Particles For Enhanced Energy Conversion To Combat Terrorism, William J. Desisto
University of Maine Office of Research Administration: Grant Reports
William J. DeSisto, University of Maine, will apply atomic layer deposition reactions to passivate nanoparticles. These coated nanoparticles will be used in next-generation, high energy density, compact energy storage devices. Initial experiments will focus on coating nano-sized lithium-ion battery anodes with titanium nitride. Miniaturized batteries using these anodes will be fabricated and evaluated by industrial collaborators at Yardley/Lithion Technical Products.
This award is supported jointly by the NSF Directorate for Mathematical and Physical Sciences and the Intelligence Community. The Approaches to Combat Terrorism Program supports new concepts in basic research and workforce development with the potential to contribute to national …
Macromolecules In Graz, Austria, December 3, 2004, Otto Vogl, Volker Ribitsch
Macromolecules In Graz, Austria, December 3, 2004, Otto Vogl, Volker Ribitsch
Emeritus Faculty Author Gallery
No abstract provided.
Solids Flow Mapping In A High Pressure Slurry Bubble Column, Novica Rados, Ashfaq Shaikh, Muthanna H. Al-Dahhan
Solids Flow Mapping In A High Pressure Slurry Bubble Column, Novica Rados, Ashfaq Shaikh, Muthanna H. Al-Dahhan
Chemical and Biochemical Engineering Faculty Research & Creative Works
Successful Design and Scale-Up of Slurry Bubble Column Reactors (SBCRs) Require Proper Understanding of How Operating Conditions Affect their Flow Behavior. Presently, there is Little Information on the Flow Dynamics of Solids (E.g., Distribution of Velocities and Turbulent Parameters) in Slurry Systems that Are Operated at Industrially Relevant Conditions of High Pressure, High Superficial Gas Velocities, and High Solids Loading. Computer Automated Radio Particle Tracking (CARPT) is Widely Recognized as One of a Few Techniques that Can Be Reliably Used Even in Highly Turbulent and Opaque Slurry Flows. This Work Utilizes an Improved CARPT Technique to Investigate the Effect of …
Coupling Between Helix-Coil And Coil-Globule Transitions In Helical Polymers, Vikas Varshney, Gustavo A. Carri
Coupling Between Helix-Coil And Coil-Globule Transitions In Helical Polymers, Vikas Varshney, Gustavo A. Carri
College of Polymer Science and Polymer Engineering
We explore the coupling between the helix-coil and coil-globule transitions of a helical polymer using Monte Carlo simulations. A very rich state diagram is found. Each state is characterized by a specific configuration of the chain which could be a helix, a random coil, an amorphous globule, or one of various other globular states which carry residual helical strands. We study the boundaries between states and provide further insight into the physics of the system with a detailed analysis of the order parameter and other properties.
Royal Society Of Chemistry - Gold-Plated Bacteria, Ravi F. Saraf, Vikas Berry
Royal Society Of Chemistry - Gold-Plated Bacteria, Ravi F. Saraf, Vikas Berry
Department of Chemical and Biomolecular Engineering: News Releases
No Abstract
Confinement-Induced Ordering Of Alkanes Between An Elastomer And A Solid Surface, Kumar Nanjundiah, Ali Dhinojwala
Confinement-Induced Ordering Of Alkanes Between An Elastomer And A Solid Surface, Kumar Nanjundiah, Ali Dhinojwala
College of Polymer Science and Polymer Engineering
We have studied the molecular structure of liquid alkanes confined between a flexible elastomeric poly(dimethyl siloxane) lens and a rigid sapphire substrate using surface-sensitive infrared-visible sum frequency generation spectroscopy. The reduction in the gauche defects suggests ordering of liquid alkanes under confinement. The cooling of confined liquid below the freezing temperature leads to crystallization with alkane molecules lying on the substrate with the symmetry axis parallel to the surface normal. This structure is very different from the bulk alkane crystals next to sapphire or air interfaces.
The Role Of Cavitation In Acoustically Activated Drug Delivery, Mario A. Diaz, Ghaleb A. Husseini, William G. Pitt, Eric S. Richardson, Douglas A. Christensen
The Role Of Cavitation In Acoustically Activated Drug Delivery, Mario A. Diaz, Ghaleb A. Husseini, William G. Pitt, Eric S. Richardson, Douglas A. Christensen
Faculty Publications
The triblock copolymer, Pluronic P105, has been found to be an ideal ultrasonically activated drug delivery vehicle because it forms micelles with hydrophobic polypropylene oxide cores that sequester hydrophobic drugs (Fig. 1). These micelles release their contents upon the application of low frequency ultrasound [1]such that drugs can be released specifically at the ultrasonicated region (Fig. 2). Such ultrasonically controlled release has been effective against cancer cells in vitro [2] and in vivo [3]. This poster presents our results showing that collapse cavitation is associated with drug release. Cavitation is generally divided into two types of behavior. Stable cavitation is …
Nature - Nanotechnology-Live Wire, Ravi F. Saraf, Vikas Berry
Nature - Nanotechnology-Live Wire, Ravi F. Saraf, Vikas Berry
Department of Chemical and Biomolecular Engineering: News Releases
No Abstact.
Phase Stability Analysis Using Interval Newton Method With Nrtl Model, Hatice Gecegormez, Yaşar Demirel
Phase Stability Analysis Using Interval Newton Method With Nrtl Model, Hatice Gecegormez, Yaşar Demirel
Papers in Fluid Mechanics
The Gibbs energy minimization using activity coefficient models and nonlinear equation solution techniques are commonly applied for phase stability problems. However, dependence on the initial estimates and multiple solutions for these highly nonlinear equations are common drawbacks for some of the conventional approaches. We have used interval Newton method with the local composition model of NRTL for the phase stability analysis of 10 binary systems and 2 ternary systems at various feed compositions to locate all the stationary points. Results indicate that the interval Newton method is reliable and efficient.
Enhancement Of Protein Secretion In Pichia Pastoris By Overexpression Of Protein Disulfide Isomerase, Mehmet Inan, Dinesh Aryasomayajula, Jayanta Sinha, Michael M. Meagher
Enhancement Of Protein Secretion In Pichia Pastoris By Overexpression Of Protein Disulfide Isomerase, Mehmet Inan, Dinesh Aryasomayajula, Jayanta Sinha, Michael M. Meagher
Papers in Biochemical Engineering
A potential vaccine candidate, Necator americanus secretory protein (Na-ASP1), against hookworm infections, has been expressed in Pichia pastoris. Na- ASP1, a 45 kDa protein containing 20 cysteines, was directed outside the cell by fusing the protein to the preprosequence of the a-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene. Overexpression of the endoplasmic reticulum (ER) resident, homologous chaperone protein, …
Effect Of Thermal Transport On Spatiotemporal Emergence Of Lamellar Branching Morphology During Polymer Spherulitic Growth, Haijun Xu, Wirunya Keawwattana, Thein Kyu
Effect Of Thermal Transport On Spatiotemporal Emergence Of Lamellar Branching Morphology During Polymer Spherulitic Growth, Haijun Xu, Wirunya Keawwattana, Thein Kyu
College of Polymer Science and Polymer Engineering
Spatiotemporal emergence of lamellar branching morphology of polymer spherulite has been investigated theoretically in the framework of a phase field model by coupling a crystal solidification potential pertaining to a nonconserved crystal order parameter with a temperature field generated by latent heat of crystallization. A local free-energy density having an asymmetric double well has been utilized to account for a first-order phase transition such as crystallization. To account for the polymorphous nature of polymer crystallization, the phase field order parameter of crystal at the solidification potential of the double-well local free-energy density is modified to be supercooling dependent. The heat …
Cgmp Recombinant Fix For Iv And Oral Hemophilia B Therapy, William H. Velander Principal Investigator, Abramson Stephan Lead Investigator, Cooper Julian Lead Investigator, William Dernell Key Person, Mark Manning Lead Investigator, Michael Meagher Lead Investigator, Paul Mohanan Lead Investigator, Timothy Nichols Lead Investigator, Todd Swaanson Key Person, Kevin E. Van Cott
Cgmp Recombinant Fix For Iv And Oral Hemophilia B Therapy, William H. Velander Principal Investigator, Abramson Stephan Lead Investigator, Cooper Julian Lead Investigator, William Dernell Key Person, Mark Manning Lead Investigator, Michael Meagher Lead Investigator, Paul Mohanan Lead Investigator, Timothy Nichols Lead Investigator, Todd Swaanson Key Person, Kevin E. Van Cott
Department of Chemical and Biomolecular Engineering: Funded Proposals
Three specific aims are proposed: Specific Aim # 1. Process engineer and scale-up the recovery and purification of transgenic recombinant human Factor IX. The University of Nebraska-Lincoln Biological Process Development Facility will complete process development and scale-up, and produce clinical grade materials for preclinical studies. The endpoint is a proposed final product specification to help facilitate transfer to current Good Manufacturing Practices compliant production of clinical grade material to support an Investigational New Drug filing with the United States Food and Drug Administration (FDA) leading to clinical trials. Specific Aim #2. Characterize and formulate transgenic recombinant human Factor IX for …
Rupture Of A Two-Dimensional Alkane Crystal, Shishir Prasad, Ali Dhinojwala
Rupture Of A Two-Dimensional Alkane Crystal, Shishir Prasad, Ali Dhinojwala
College of Polymer Science and Polymer Engineering
We have studied the breaking of a two-dimensional alkane crystal above the disordered melt using an oscillating bubble rheometer. Surface tension changes abruptly during the expansion and contraction cycle. We postulate that this is due to rupture of the 2D crystal at grain boundaries. The magnitude of the abrupt change in surface tension decreases with a decrease in the rate of change of bubble surface area with a power law exponent of 0.8. The interfacial area formed after rupture decreases with a decrease in rate. These results provide new insights in understanding defect-mediated rupture in confined geometry.
Branching In Electrospinning Of Nanofibers, A. L. Yarin, W. Kataphinan, Darrell Hyson Reneker
Branching In Electrospinning Of Nanofibers, A. L. Yarin, W. Kataphinan, Darrell Hyson Reneker
College of Polymer Science and Polymer Engineering
Electrospinning of polymer nanofibers often begins with a single, straight, elongating, and electrified fluid jet that emanates from a droplet tip when the electric field at the surface is high enough. After some distance an electrically driven bending instability of the elongating jet occurs. For a polymer solution suitable for electrospinning, capillary instability does not cause the jet to become a spray of droplets. Under some conditions, a sequence of secondary jet branches emanates from the primary jet. This paper describes an experiment in which many closely spaced branches along the jet were observed during the electrospinning of a polycaprolactone …
Adsorption Of Lactic Acid From Fermentation Broth And Aqueous Solutions On Zeolite Molecular Sieves, Isam H. Aljundi, Joanne M. Belovich, Orhan Talu
Adsorption Of Lactic Acid From Fermentation Broth And Aqueous Solutions On Zeolite Molecular Sieves, Isam H. Aljundi, Joanne M. Belovich, Orhan Talu
Chemical & Biomedical Engineering Faculty Publications
The recovery of lactic acid from fermentation broth and aqueous solutions was studied by adsorption on Silicalite molecular sieves. Batch experiments were used to measure the adsorption isotherms of the lactic acid on Silicalite. A linear correlation was found for both solutions. Silicalite showed a higher adsorptive capacity in the case of the aqueous solution than that of the fermentation broth. Henry’s constants were estimated as Formula Not Shown and Formula Not Shown for the aqueous and broth solutions, respectively. The effect of temperature on adsorption was also studied in batch mode. Henry’s constant dependency on …
The Processing, Mechanical Properties And Bioactivity Of Zinc Based Glass Ionomer Cements, D. Boyd, Mark R. Towler
The Processing, Mechanical Properties And Bioactivity Of Zinc Based Glass Ionomer Cements, D. Boyd, Mark R. Towler
Chemical and Biochemical Engineering Faculty Research & Creative Works
The suitability of Glass Ionomer Cements (GICs) for use in orthopaedics is retarded by the presence in the glass phase of aluminium, a neurotoxin. Unfortunately, the aluminium ion plays an integral role in the setting process of a GIC and its absence is likely to hinder cement formation. However, zinc oxide, a bacteriocide, can act both as a network modifying oxide and an intermediate oxide in a similar fashion to alumina and so ternary systems based on zinc silicates often have extensive regions of glass formation. The purpose of this research was to produce novel GICs based on calcium zinc …
Under Graduate Course Description
Under Graduate Course Description
Department of Chemical and Biomolecular Engineering: Courses
No abstract provided.
Exfoliated Graphite Nanofibers For Hydrogen Storage, Angela D. Lueking, Ling Pan, Deepa L. Narayanan, Caroline E. Burgess Clifford
Exfoliated Graphite Nanofibers For Hydrogen Storage, Angela D. Lueking, Ling Pan, Deepa L. Narayanan, Caroline E. Burgess Clifford
Chemical and Biochemical Engineering Faculty Research & Creative Works
Exfoliation of graphite nanofibers (GNF) expands the interplanar spacing of the GNF, which leads to increased hydrogen storage. Hydrogen uptake measurements at 20 bar indicated that the overall hydrogen uptake and operative adsorption temperature are sensitive to the structural variations and graphitic spacing. The increased surface area of the EGNF-1000 led to a 1.2% hydrogen uptake at 77 K and 20 bar, a three-fold increase in hydrogen physisorption of the starting material. These results suggested that selective exfoliation of a nanofiber is a means by which to control the relative binding energy of the hydrogen interaction with the carbon structure …
A Global Regulatory Role Of Gluconeogenic Genes In Escherichia Coli Revealed By Transcriptome Network Analysis, Katy Kao, Linh Tran, James Liao
A Global Regulatory Role Of Gluconeogenic Genes In Escherichia Coli Revealed By Transcriptome Network Analysis, Katy Kao, Linh Tran, James Liao
Faculty Publications
In bacterial adaptation to the dynamic environment, metabolic genes are typically thought to be the executors, whereas global transcription regulators are regarded as the decision makers. Although the feedback from metabolic consequence is believed to be important, much less is understood. This work demonstrates that the gluconeogenic genes in Escherichia coli, ppsA, sfcA, and maeB, provide a feedback loop to the global regulator, cAMP receptor protein (CRP), in carbon source transition. Disruption of one of the gluconeogenic pathways has no phenotype in balanced growth, but causes a significant delay in the diauxic transition from glucose to acetate. To investigate the …
Crosslinked Chitosan: Its Physical Properties And The Effects Of Matrix Stiffness On Chondrocyte Cell Morphology And Proliferation, Anuradha Subramanian, Hsin-Yi Lin
Crosslinked Chitosan: Its Physical Properties And The Effects Of Matrix Stiffness On Chondrocyte Cell Morphology And Proliferation, Anuradha Subramanian, Hsin-Yi Lin
Papers in Biotechnology
Chitosan [<(1-4)-2 amino-2-deoxy-d-glucose], the natural polyaminosaccharide derived from N-deacetylation of chitin [<(1-4)-2 acetamide-2-deoxy-d-glucose], has been shown to possess attractive biological and cell interactive properties. Recently chitosan and chitosan analogs have also been shown to support the growth and continued function of chondrocytes. In the present study, chitosan substrates are crosslinked with a func-tional diepoxide (1,4 butanediol diglycidyl ether) to alter its mechanical property, and the viability and proliferation of the canine articular chondrocytes seeded on the crosslinked surface are further assayed. Of interest is the impact of substrate stiffness on the growth and proliferation of articular canine chondrocytes. Cross linked scaffolds were also sub-jected to degradation by chitosanase to examine the impact of cross linking on enzyme- assisted degradation. The hydrophilicity and compression modulus of the crosslinked sur-faces were measured via contact-angle measurements and compression tests, respec-tively. Scanning electron microscopy (SEM) and fluorescent staining were used to ob-serve the proliferation and morphology of chondrocyte cells on noncrosslinked and crosslinked surfaces. The crosslinked chitosan was found to be nontoxic to chondrocytes and more hydrophilic. Its compression modulus and stiffness increased, which may im-prove the scaffold resistance to wear and in vivo shrinkage once implanted. The in-creased stiffness also seemed to serve as an additional mechanical stimulus to promote chondrocyte growth and proliferation. The cell morphology on crosslinked scaffolds seen by SEM and fluorescent stain was the typical chondrocytic rounded shape. The method proposed provides a nontoxic way to increase the mechanical strength of the chitosan scaffolds.
Purification And Scale-Up Of A Recombinant Heavy Chain Fragment C Of Botulinum Neurotoxin Serotype E In Pichia Pastoris Gs115, Michael P. Dux, Rick Barent, Jayanta Sinha, Mark Gouthro, Todd Swanson, Ardis Barthuli, Mehmet Inan, John T. Ross, Leonard A. Smith, Theresa J. Smith, Robert Webb, Bonnie Loveless, Ian Henderson, Michael M. Meagher
Purification And Scale-Up Of A Recombinant Heavy Chain Fragment C Of Botulinum Neurotoxin Serotype E In Pichia Pastoris Gs115, Michael P. Dux, Rick Barent, Jayanta Sinha, Mark Gouthro, Todd Swanson, Ardis Barthuli, Mehmet Inan, John T. Ross, Leonard A. Smith, Theresa J. Smith, Robert Webb, Bonnie Loveless, Ian Henderson, Michael M. Meagher
Papers in Biochemical Engineering
A recombinant C-terminus heavy chain fragment from botulinum neurotoxin serotype E (BoNT/E) is proposed as a vaccine against the serotype E neurotoxin. This fragment, rBoNTE(Hc), was produced intracellular in Pichia pastoris GS115 by a three-step fermentation process, i.e., glycerol batch phase and a glycerol fed-batch phase to achieve high cell densities, followed by a methanol fed-batch induction phase. The rBoNTE(Hc) protein was purified from the soluble fraction of cell lysates using three ion-exchange chromatography steps (SP Sepharose Fast Flow, Q Sepharose Fast Flow, Sp Sepharose High Performance) and polished with a hydrophobic charge induction chromatography step (MEP HyperCel). Method development …
Modeling Catalytic Trickle-Bed And Upflow Packed-Bed Reactors For Wet Air Oxidation Of Phenol With Phase Change, Jing Guo, Muthanna H. Al-Dahhan
Modeling Catalytic Trickle-Bed And Upflow Packed-Bed Reactors For Wet Air Oxidation Of Phenol With Phase Change, Jing Guo, Muthanna H. Al-Dahhan
Chemical and Biochemical Engineering Faculty Research & Creative Works
In This Study, to Simulate the Steady-State Behavior of Packed-Bed Reactors for Catalytic Wet Oxidation of Phenol, One-Dimensional (1D) Axial Dispersion Model for the Liquid Phase is Coupled with a Cell Stack Model for the Gas Phase, Providing Considerable Phase Change under the Selected Operating Conditions. the Reactor Scale Governing Equations, Reaction Kinetics Involved, and Solution Strategy Are Discussed. the Computational Approach Accounts for the Observed Catalyst Activities, Combined with Local Transport and Catalyst Wetting Effects. the Approach Selected is Shown to Be Suitable and Efficient in Dealing with the Problem in Question. Comparisons of Simulated Model Predictions and Lab …