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Full-Text Articles in Chemical Engineering
Ranque–Hilsch Vortex Tube Thermocycler For Fast Dna Amplification And Real-Time Optical Detection, Ryan J. Ebmeier, Scott E. Whitney, Amitabha Sarkar, Michael Nelson, Nisha Padhye, George Gogos, Hendrik J. Viljoen
Ranque–Hilsch Vortex Tube Thermocycler For Fast Dna Amplification And Real-Time Optical Detection, Ryan J. Ebmeier, Scott E. Whitney, Amitabha Sarkar, Michael Nelson, Nisha Padhye, George Gogos, Hendrik J. Viljoen
Papers in Biochemical Engineering
An innovative polymerase chain reaction (PCR) thermocycler capable of performing real-time optical detection is described below. This device utilizes the Ranque–Hilsch vortex tube in a system to efficiently and rapidly cycle three 20 mL samples between the denaturation, annealing, and elon-gation temperatures. The reaction progress is displayed real-time by measuring the size of a fluo-rescent signal emitted by SYBR green/double-stranded DNA complexes. This device can produce significant reaction yields with very small amounts of initial DNA, for example, it can amplify 0.25 fg (,5 copies) of a 96 bp bacteriophage l-DNA fragment 2.731011-fold by performing 45 cycles in less than …
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Methylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Mehmet Inan, Michael M. Meagher, Bradley A. Plantz, Jayanta Sinha
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Methylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Mehmet Inan, Michael M. Meagher, Bradley A. Plantz, Jayanta Sinha
Papers in Biochemical Engineering
It was observed that during fermentative production of recombinant ovine interferon-H (r-oIFN-H ) in Pichia pastoris, a secreted recombinant protein, the protein was degraded increasingly after 48 h of induction and the rate of degradation increased towards the end of fermentation at 72 h, when the fermentation was stopped. Proteases, whose primary source was the vacuoles, was found in increasing levels in the cytoplasm and in the fermentation broth after 48 h of induction and reached maximal values when the batch was completed at 72 h. Protease levels at various cell fractions as well as in the culture supernatant were …