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Optimization Of Sequential Purification Of Beta-Glucosidase From Tricoderma Reesei In Aqueous Two-Phase System, Shalini Gautam
Optimization Of Sequential Purification Of Beta-Glucosidase From Tricoderma Reesei In Aqueous Two-Phase System, Shalini Gautam
Dissertations
A novel sequential technique was developed for the purification of a valuable enzyme, beta-glucosidase, from microorganism Tricoderma reesei. The fungus T. reesei produces cellulose degrading enzymes, called cellulases: beta-glucosidase, endo-glucanase and exo-glucanase and low molecular weight proteins. For specific applications, the enzyme must be separated from other contaminants. The sequential technique, that included affinity precipitation with chitosan followed by separation with an aqueous two-phase system (ATPS), was implemented for the purification of beta-glucosidase from the culture filtrate of T. reesei.
The cultivation medium (nutrient) was optimized for the production of betaglucosidase from T. reesei cell culture. Treatment of the …
Studies On Improved Integrated Membrane-Based Chromatographic Process For Bioseparation, Yanke Xu
Studies On Improved Integrated Membrane-Based Chromatographic Process For Bioseparation, Yanke Xu
Dissertations
To improve protein separation and purification directly from a fermentation broth, a novel membrane filtration-cum-chromatography device configuration having a relatively impermeable coated zone near the hollow fiber module outlet has been developed. The integrated membrane filtration-cum-chromatography unit packed with chromatographic beads on the shell side of the hollow fiber unit enjoys the advantages of both membrane filtration and chromatography; it allows one to load the chromatographic media directly from the fermentation broth or lysate and separate the adsorbed proteins through the subsequent elution step in a cyclic process.
Interfacial polymerization was carried out to coat the bottom section of the …