Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Chinese hamster ovary; CHO; Mammalian; Cell culture; Zirconia; HCIC; Botulism; Botulism neurotoxin; BIAcore; Antibody (1)
- Degree of coupling (1)
- Dissipation (1)
- Exergy (1)
- Factor IX (1)
-
- Haemophilia (1)
- Interferon ? (1)
- Interferon-H ; methanol; Pichia pastoris (1)
- Model (1)
- Nonequilibrium thermodynamics (1)
- Oxidative phosphorylation (1)
- Pichia pastoris Æ Continuous fermentation Optimization (1)
- Proteolytic degradation; secreted recombinant (1)
- Proteolytic degradation; secreted recombinant interferon-T ; methanol; Pichia pastoris (1)
- Transgenic animal (1)
Articles 1 - 7 of 7
Full-Text Articles in Chemical Engineering
Ranque–Hilsch Vortex Tube Thermocycler For Fast Dna Amplification And Real-Time Optical Detection, Ryan J. Ebmeier, Scott E. Whitney, Amitabha Sarkar, Michael Nelson, Nisha Padhye, George Gogos, Hendrik J. Viljoen
Ranque–Hilsch Vortex Tube Thermocycler For Fast Dna Amplification And Real-Time Optical Detection, Ryan J. Ebmeier, Scott E. Whitney, Amitabha Sarkar, Michael Nelson, Nisha Padhye, George Gogos, Hendrik J. Viljoen
Papers in Biochemical Engineering
An innovative polymerase chain reaction (PCR) thermocycler capable of performing real-time optical detection is described below. This device utilizes the Ranque–Hilsch vortex tube in a system to efficiently and rapidly cycle three 20 mL samples between the denaturation, annealing, and elon-gation temperatures. The reaction progress is displayed real-time by measuring the size of a fluo-rescent signal emitted by SYBR green/double-stranded DNA complexes. This device can produce significant reaction yields with very small amounts of initial DNA, for example, it can amplify 0.25 fg (,5 copies) of a 96 bp bacteriophage l-DNA fragment 2.731011-fold by performing 45 cycles in less than …
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Ethylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Jayanta Sinha, Bradley A. Plantz, Mehmet Inan, Michael Meagher
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Ethylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Jayanta Sinha, Bradley A. Plantz, Mehmet Inan, Michael Meagher
Papers in Biotechnology
It was observed that during fermentative production of recombinant ovine interferon-H (r-oIFN-H ) in Pichia pastoris, a secreted recombinant protein, the protein was degraded increasingly after 48 h of induction and the rate of degradation increased towards the end of fermentation at 72 h, when the fermentation was stopped. Proteases, whose primary source was the vacuoles, was found in in-creasing levels in the cytoplasm and in the fermentation broth after 48 h of induction and reached maximal values when the batch was completed at 72 h. Protease levels at various cell fractions as well as in the culture supernatant were …
Exergy Use In Bioenergetics, Yaşar Demirel
Exergy Use In Bioenergetics, Yaşar Demirel
Papers in Analytical Chemistry
Every developed and adapted biological system extracts useful energy from outside, converts, stores it, and uses for muscular contraction, substrate transport, protein synthesis, and other energy utilizing processes. This energy management in a living cell is called the bioenergetics, and the useful energy is the exergy, which is destroyed in every irreversible process because of the entropy production. The converted exergy is the adenosine triphosphate (ATP) produced through the oxidative phosphorylation coupled to respiration in which the exergy originates from oxidation of reducing equivalents of nutrients. A living cell uses the ATP for all the energy demanding activities; it has …
Haemophilic Factors Produced By Transgenic Livestock: Abundance That Can Enable Alternative Therapies Worldwide, Kevin E. Van Cott, Paul E. Monahan, Timothy C. Nichols, William H. Velander
Haemophilic Factors Produced By Transgenic Livestock: Abundance That Can Enable Alternative Therapies Worldwide, Kevin E. Van Cott, Paul E. Monahan, Timothy C. Nichols, William H. Velander
Papers in Biotechnology
Haemophilia replacement factors, both plasma-derived and recombinant, are in relatively short supply and are high-cost products. This has stymied the study and development of alternative methods of administration of haemophilia therapy even in the most economically advanced countries, owing to the large amounts of material needed because bioabsorption and bioavailability of haemophilic factors can be less than 10% when using non-intravenous routes of delivery. There is therefore a need to increase access to therapy worldwide by decreasing the cost and increasing the abundance so that therapy can be achieved through simplified, alternative delivery methods. Transgenic livestock have been used to …
Production And Purification Of A Chimeric Monoclonal Antibody Against Botulinum Neurotoxin Serotype A , Mark C. Mowry, Michael Meagher Lead Investigator, Leonard Smith, Anuradha Subramanian
Production And Purification Of A Chimeric Monoclonal Antibody Against Botulinum Neurotoxin Serotype A , Mark C. Mowry, Michael Meagher Lead Investigator, Leonard Smith, Anuradha Subramanian
Papers in Biotechnology
Production of recombinant antibodies against botulinum neurotoxin is necessary for the development of a post-exposure treatment. CHO-DG44 cells were transfected with a plasmid encoding the light and heavy chains of a chimeric monoclonal antibody (S25) against botulism neurotoxin serotype A. Stable cell lines were obtained by dilution cloning and clones were shown to produce nearly equivalent levels of light and heavy chain antibody by an enzyme-linked immunosorbent assay (ELISA). In suspension culture, cells produced 35 μg/ml of chimeric antibody after 6 days, corresponding to a specific antibody productivity of 3.1 pg/cell/day. A method for the harvest and recovery of an …
Optimization Of Cell Density And Dilution Rate In Pichia Pastoris Continuous Fermentations For Production Of Recombinant Proteins, Mehmet Inan, Michael M. Meagher, Wenhui Zhang, Chih Ping Liu
Optimization Of Cell Density And Dilution Rate In Pichia Pastoris Continuous Fermentations For Production Of Recombinant Proteins, Mehmet Inan, Michael M. Meagher, Wenhui Zhang, Chih Ping Liu
Papers in Biomolecular Engineering
This paper provides an approach for optimizing the cell density (Xc) and dilution rate (D) in a chemostat for a Pichia pastoris continuous fermentation for the extracellular production of a recombinant protein, interferon τ (INF-τ). The objective was to maximize the volumetric productivity (Q, mg INF-τ I-1 h-1), which was accomplished using response surface methodology (RSM) to model the response of Q as a function of Xc and D within the ranges 150 ≤ Xc ≤ 450 g cells (wet weight) l-1 and 0.1 μm ≤D ≤ 0.9 μm (μm =0.0678 h-1, the maximum specific growth rate obtained from a …
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Methylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Mehmet Inan, Michael M. Meagher, Bradley A. Plantz, Jayanta Sinha
Causes Of Proteolytic Degradation Of Secreted Recombinant Proteins Produced In Methylotrophic Yeast Pichia Pastoris: Case Study With Recombinant Ovine Interferon-T, Mehmet Inan, Michael M. Meagher, Bradley A. Plantz, Jayanta Sinha
Papers in Biochemical Engineering
It was observed that during fermentative production of recombinant ovine interferon-H (r-oIFN-H ) in Pichia pastoris, a secreted recombinant protein, the protein was degraded increasingly after 48 h of induction and the rate of degradation increased towards the end of fermentation at 72 h, when the fermentation was stopped. Proteases, whose primary source was the vacuoles, was found in increasing levels in the cytoplasm and in the fermentation broth after 48 h of induction and reached maximal values when the batch was completed at 72 h. Protease levels at various cell fractions as well as in the culture supernatant were …