Bioresource and Agricultural Engineering Commons^™

Low-Level Groundwater Atrazine In High Atrazine Usage Nebraska Counties: Likely Effects Of Excessive Groundwater Abstraction, Moses New-Aaron, Olufemi P. Abimbola, Raheleh Mohammadi, Oluwaseun Famojuro, Zaeema Naveed, Azar M Abadi, Jesse E. Bell, Shannon Bartelt-Hunt, Eleanor G. Rogan

Biological Systems Engineering: Papers and Publications

Recent studies observed a correlation between estrogen-related cancers and groundwater atrazine in eastern Nebraska counties. However, the mechanisms of human exposure to atrazine are unclear because low groundwater atrazine concentration was observed in counties with high cancer incidence despite having the highest atrazine usage. We studied groundwater atrazine fate in high atrazine usage Nebraska counties. Data were collected from Quality Assessed Agrichemical Contaminant Nebraska Groundwater, Parameter–Elevation Regressions on Independent Slopes Model (PRISM), and water use databases. Descriptive statistics and cluster analysis were performed. Domestic wells (59%) were the predominant well type. Groundwater atrazine was affected by well depth. Clusters consisting …

Gis-Based Volunteer Cotton Habitat Prediction And Plant-Level Detection With Uav Remote Sensing, Tianyi Wang, Xiaohan Mei, J. Alex Thomasson, Chenghai Yang, Xiongzhe Han, Pappu Kumar Yadav, Yeyin Shi

Biological Systems Engineering: Papers and Publications

Volunteer cotton plants germinate and grow at unwanted locations like transport routes and can serve as hosts for a harmful cotton pests called cotton boll weevils. The main objective of this study was to develop a geographic information system (GIS) framework to efficiently locate volunteer cotton plants in the cotton production regions in southern Texas, thus reducing time and economic cost for their removal. A GIS network analysis tool was applied to estimate the most likely routes for cotton transportation, and a GIS model was created to identify and visualize potential areas of volunteer cotton growth. The GIS model indicated …

Global Analysis Of Differential Gene Expression Within The Porcine Conceptus Transcriptome As It Transitions Through Spherical, Ovoid, And Tubular Morphologies During The Initiation Of Elongation, Sophie C. Walsh, Jeremy R. Miles, Brittney N. Keel, Lea A. Rempel, Elane C. Wright‐Johnson, Amanda K. Lindholm‐Perry, William T. Oliver, Angela K. Pannier

Biological Systems Engineering: Papers and Publications

This study aimed to identify transcriptome differences between distinct or transitional stage spherical, ovoid, and tubular porcine blastocysts throughout the initiation of elongation. We performed a global transcriptome analysis of differential gene expression using RNA‐Seq with high temporal resolution between spherical, ovoid, and tubular stage blastocysts at specific sequential stages of development from litters containing conceptus populations of distinct or transitional blastocysts. After RNA‐Seq analysis, significant differentially expressed genes (DEGs) and pathways were identified between distinct morphologies or sequential development stages. Overall, 1898 significant DEGs were identified between distinct spherical and ovoid morphologies, with 311 total DEGs between developmental stages …

Injectable Decellularized Nucleus Pulposus Tissue Exhibits Neuroinhibitory Properties, Logan M. Piening, David J. Lillyman, Fei San Lee, Alvaro Moreno Lozano, Jeremy R. Miles, Rebecca A. Wachs

Biological Systems Engineering: Papers and Publications

Background: Chronic low back pain (LBP) is a leading cause of disability, but treatments for LBP are limited. Degeneration of the intervertebral disc due to loss of neuroinhibitory sulfated glycosaminoglycans (sGAGs) allows nerves from dorsal root ganglia to grow into the core of the disc. Treatment with a decellularized tissue hydrogel that contains sGAGs may inhibit nerve growth and prevent discassociated LBP.

Methods: A protocol to decellularize porcine nucleus pulposus (NP) was adapted from previous methods. DNA, sGAG, α-gal antigen, and collagen content were analyzed before and after decellularization. The decellularized tissue was then enzymatically modified to be …

Irrigation Systems Management, Dean Eisenhauer, Derrel L. Martin, Derek M. Heeren, Glenn J. Hoffman

Biological Systems Engineering: Papers and Publications

Management of irrigation systems should be based on the desired objectives or outcomes consistent with economic, energy, environmental, labor, water, and resource constraints. Goals can vary from maximizing profit, producing a contracted yield, optimizing water resource use, maintaining the quality of produce, or assuring an attractive landscape. Managers cannot achieve these goals without considering the performance of the irrigation system. This chapter discusses the basic characteristics of various irrigation systems, defines terms that quantify performance, describes basic requirements all systems must provide, gives a range of attributes for systems, and discusses how water supply requirements are governed by ET and …

Development And Characterization Of A Decellularized Neuroinhibitory Scaffold Containing Matrix Bound Nanovesicles, Logan Piening

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Chronic low back pain (LBP) is a leading cause of disability but treatments for LBP are limited. Degeneration of the intervertebral disc leads to loss of neuroinhibitory sulfated glycosaminoglycans (sGAGs) which allows nerves from dorsal root ganglia (DRG) to grow into the core of the disc, leading to pain. Current treatments for LBP involve drugs that do not target the source of the pain and lack long term efficacy or use invasive surgeries with high complication rates. Treatment with a decellularized tissue scaffold that contains neuroinhibitory components may inhibit nerve growth and prevent disc-associated LBP. Here, a decellularized nucleus pulposus …

The Application Of Mathematical Optimization And Flavor-Detection Technologies For Modeling Aroma Of Hops, Yutong Liu

Department of Food Science and Technology: Dissertations, Theses, and Student Research

In recent years, proprietary hops (Citra, Simcoe, and Mosaic) become the most sought-after hops among brewers due to their excellent aroma. However, they are restricted to the owners unless other growers purchase the costly licensing agreements. Many public hops are available to the growers without any additional costs, but their aroma is difficult to match to the proprietary hops. Although proprietary and public hop varieties are unique in their aroma profiles, all hops varieties contain similar volatile compounds, merely differ in the quantity of different individual compounds. The main objective of this thesis was to investigate the feasibility of matching …

High-Frequency Unmanned Aircraft Flights For Crop Canopy Imaging During Diurnal Moisture Stress, Suresh Pradhyun Kashyap

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Previous research has used unmanned aerial vehicle (UAV) technology for calculating CWSI (Crop Water Stress Index) values in the context of irrigation scheduling. Typically, these estimations were taken at one time of day, usually near shortly after solar noon. A significant limitation with these CWSI values is that the UAV thermal imagery captured at this point in time can be affected by various factors like atmospheric air temperature, solar radiation, wind speed, relative humidity, and other micrometeorological disturbances in the air. In order to address these temporal effects, high-frequency UAV flights were conducted over different daylight hours to analyze and …

Evaluation Of A Novel Approach For Assessing Biological Activity In Agricultural Soils, Karla Melgar

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Soil health is a key factor impacting soil resilience and fertility in crop production systems. Favorable soil physical and biological conditions facilitate plant nutrient absorption and nutrient cycling. Demonstrating to farmers the impacts and changes in soil biological activity under different soil management practices has been a challenge due to the limited availability of inexpensive tools for quantifying this component of soil health. The primary goal of this study was to present a simple and readily accessible tool for evaluating soil biological activity to promote the use of organic amendments in crop fields. Research plots were established in two studies …

Preferential Flow In Riparian Buffers: Current Research And Future Needs., Derek M. Heeren, Lucie Guertault, Kyle Mankin

Biological Systems Engineering: Papers and Publications

Preferential flow in riparian buffers can substantially compromise their effectiveness in reducing contaminants from overland runoff. The objective of this article is to introduce a collection of five articles on current research into subsurface preferential flow measurement, visualization, modeling, and impacts on contaminant fate and transport at scales ranging from the subsurface pore scale to the plot scale to the watershed scale. This collection presents selected works from a broader invited session on “Preferential flow and piping in riparian buffers” at the 2020 ASABE Annual International Meeting. Major findings include: new methodologies, such as light transmission and geophysics, to characterize …

Sprinkler Irrigation System Field Checklist, Charles C. Hillyer, R. Troy Peters, Xin Qiao, Derek M. Heeren, Jake Larue, Clarence Prestwich, Sandeep Bhatti

Biological Systems Engineering: Papers and Publications

This recommendation came out of a noted need by the ASABE NRES-241 sprinkler irrigation committee in 2019 committee meeting for a simple checklist to quickly educate new center pivot operators on what to look for to determine if a center pivot is operating at designed performance. This checklist is meant to be simple and user friendly. Simple and clear language was incorporated purposefully. It is intended as a quick guide to new irrigators, or as a reminder to more experienced irrigators as to what to check to ensure efficient and effective center pivot and linear move irrigation system operation for …

Corn Fiber As A Biomass Feedstock For Production Of Succinic Acid, Lisbeth Vallecilla-Yepez, Divya Ramchandran, Dianna Long, Rajib Saha, Mark R. Wilkins

Biological Systems Engineering: Papers and Publications

The selection of an economical carbon source is a fundamental parameter to establish a successful industrial succinic acid (SA) bioprocess. In this work, corn fiber (CF), a renewable and an inexpensive source of carbohydrates, was successfully used for bioproduction of SA. Optimized liquid hot water (LHW) pretreatment followed by enzymatic hydrolysis were used to obtain corn fiber hydrolysate (CFH). Results in batch fermentation with Actinobacillus succinogenes showed that a control solution mimicking CFH produced 28.7 g/L of SA with a yield of 0.67 g SA/g sugars, while fermentation of CFH produced 27.8 g/L of SA with a yield of 0.61 …

Design And Evaluation Of Unmanned Aerial System-Based Wireless Sensor Network For Irrigation Management, Jasreman Singh

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Increasing pressure on food production, both in terms of quantity and quality, has called for intensification and modernization of the agricultural sector. The “Internet of Things” (IoT) is a highly promising technology capable of advancing agricultural operations. The IoT-based real-time monitoring of soil water status and crop canopy temperature in maize and soybean could potentially improve irrigation efficiency, leading to the profitability of field crop production and conservation of natural resources. The overall goal of the work presented here is to design, develop, and evaluate the unmanned aerial system-based wireless sensor system (UWSN) for the purpose of irrigation management by …

Greenhouse Gas Emissions And Drought Projections For Nebraska, Adam Liska

Adam Liska Papers

This lecture will focus on three issues. The first is a presentation of data from the first greenhouse gas emissions inventory for the State of Nebraska, which will be published in the coming weeks. In 2016, emissions from beef cattle made up 23% of net state emissions, and coal for electricity made up 23.7% of net state emissions. The second issue to be addressed are the impacts of the 2012 drought on agriculture in Nebraska and the region. The drought of 2012 indicates that future droughts in the 21^st century in the region can be a dominant influence on …

Inter-Relationships Between Water Depletion And Temperature Differential In Row Crop Canopies In A Sub-Humid Climate, Jasreman Singh, Yufeng Ge, Derek M. Heeren, Elizabeth Walter-Shea, Christopher M.U. Neale, Suat Irmak, Wayne Woldt, Geng Bai, Sandeep Bhatti, Mitchell S. Maguire

Biological Systems Engineering: Papers and Publications

Irrigation has a great impact on global food security as it contributes to the majority of the world’s agricultural food supply. It is essential to judiciously utilize water resources through efficient irrigation management since the majority of U.S. groundwater aquifers are rapidly depleting. Thus, quantification of the relationships between water depletion and environmental factors is important for understanding crop response to varying levels of water stresses that depletion can cause. The objectives of this research were to: 1) investigate the relationship between root zone water depletion (D_rw) and canopy temperature differential (ΔT) at different ranges of D_{rw …}

Disturbance Triggers Non-Linear Microbe–Environment Feedbacks, Aditi Sengupta, Sarah J. Fansler, Rosalie K. Chu, Robert E. Danczak, Vanessa A. Garayburu-Caruso, Lupita Renteria, Hyun-Seob Song, Jason Toyoda, Jacqueline Wells, James C. Stegen

Biological Systems Engineering: Papers and Publications

Conceptual frameworks linking microbial community membership, properties, and processes with the environment and emergent function have been proposed but remain untested. Here we refine and test a recent conceptual framework using hyporheic zone sediments exposed to wetting–drying transitions. Our refined framework includes relationships between cumulative properties of a microbial community (e.g., microbial membership, community assembly properties, and biogeochemical rates), environmental features (e.g., organic matter thermodynamics), and emergent ecosystem function. Our primary aim was to evaluate the hypothesized relationships that comprise the conceptual framework and contrast outcomes from the whole and putatively active bacterial and archaeal communities. Throughout the system we …

Making Space For Social And Emotional Learning In Science Education, Erin Ingram, Kristie Reddick, Jessica M. Honaker, Gwen A. Pearson

Biological Systems Engineering: Papers and Publications

Social-emotional learning (SEL) is known to improve student outcomes but is rarely combined with STEM. In this paper we present an action research study to examine the impact of a STEM + SEL curriculum intervention to address a real-world school conflict. One hundred sixth–eighth graders and four teachers participated in an in-person facilitation of a SEL Arthropod curriculum, DIFFERENT. After the intervention, students completed open-ended couplet statements about arthropod behavior, tarantulas, and humans designed to measure sentiment change. Answers were manually coded using inductive coding on a scale of negative (1) to positive (5). Statement sentiments significantly shifted from negative …

Assessment Of The Effects Of Airflow Conditions Related To Hop Drying, David Mabie

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Hops have been utilized by brewers for centuries to contribute bitterness, antimicrobial preservation and desirable aromatic profiles to beer. The hop cone is a small, lightweight, high moisture content flower which produces lupulin glands containing resins and essential oils that contribute desirable characteristics to beer. Due to the high harvest moisture content (75% wet basis), hops must be rapidly dried to properly preserve them for future processing and brewing. While hops have been dried for centuries, most literature from the past century has focused on drying to minimizing loss to the resin or physical cone structure. The objectives of this …

Development Of Tractor Instrumentation System: Hydraulics, And Controller Area Network (Can) Data Analysis Of Agricultural Machinery, Chee Town Liew

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The tractor is a very important piece of machinery in agriculture, and it has been evolving and progressing over the years with improved and increasingly complex functionality. Controller Area Network (CAN) has been incorporated into tractors and other machinery with SAE J1939 and ISO 11783 standards, serving as the communication bus for various on-board electronic controller units (ECUs), and carries various machine operation data that can be used for analyzing machine performance. Investigation of data extraction from CAN messages was conducted by examining the SAE J1939 and ISO 11783 standards. A case study on CAN bus data analysis for a …

Tractor Instrumentation System: Hitch And Pto, And Row Crop Tractor Electrification Considerations, Andrew Donesky

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Power sources used for vehicles are advancing at a fast pace. Electric batteries are becoming more power dense, thus allowing them to be used with electric motors in place of a diesel or gas powered systems. There are several ways that energy use and storage size can be computed for agricultural field operations, such as planting, using theoretical predictions, gathering engine load data from tractor’s Controller Area Network (CAN) bus, or integrating the CAN data to determine the actual power used by implements.

While measuring data from the CAN bus is a great way to capture actual tractor use information, …

Leveraging Unmanned Aerial System Remote Sensing To Inform Energy And Water Balance Models For Spatial Soil Water Content Monitoring And Irrigation Management, Mitchell S. Maguire

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Irrigation has provided a means to produce more food and fiber throughout the world, converting low producing land into high yielding cropping systems in certain scenarios. The managing of irrigation has taken on various approaches as different locations have been constrained by different factors. Certain areas have significant ground and surface water available for irrigation while other areas struggle to meet irrigation demands due to limited water resources. These factors, along with the desire to increase crop water use efficiency, has provided the motivation to better understand crop water demands spatially within a field. A sub-field scale irrigation management study …

Efficient Polyhydroxyalkanoate Production By Rhodopseudomonas Palustris From Lignocellulosic Biomass, Brandi Brown

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Polyhydroxyalkanoates (PHAs) are biopolymers produced by bacteria with the potential to replace conventional plastics. However, the relatively high production costs of PHAs are keeping them from market acceptance, with approximately half of the production costs derived from the feedstock. Thus, engineering a microbe for PHA production from cheaper and renewable carbon sources is necessary to promote the valorization of PHAs. Lignocellulosic biomass is considered to be one of the most economic carbon sources in the world, and is thus an attractive candidate for cheaper production of bioplastics. Rhodopseudomonas palustris CGA009 is a metabolically robust bacterium capable of catabolizing lignin breakdown …

Oxygen Reactive Polymers For Treatment Of Traumatic Brain Injury, Parick S. Stayton, Menko P. Ypma, Peter A. Chiarelli, Joshua Sang Hun Park, Richard G. Ellenbogen, Julia Mengyun Xu, Pierre D. Mourad, Donghoon Lee, Anthony Convertime, Forrest M. Kievit

Biological Systems Engineering: Papers and Publications

Methods and compositions for treating traumatic brain injury . The methods and compositions utilize a multi - functional oxygen reactive polymer ( ORP ) that includes repeating units that include a reactive oxygen species ( ROS ) scavenging group and a polyalkylene oxide group . For theranostic applications , the oxygen reactive polymer fur ther includes a diagnostic group .

Impact Of Irrigation Technologies On Withdrawals And Consumptive Use Of Water, Eric Wilkening, Derek M. Heeren, Douglas R. Hallum, Jennifer Schellpeper, Derrel Martin

Biological Systems Engineering: Papers and Publications

Developments in irrigation technologies have subsequently led to increases in irrigation application efficiency, including conversion from surface to sprinkler irrigation, variable rate irrigation (VRI), and low energy precision application. However, while these increases in application efficiency may reduce water withdrawals and/or diversions (from groundwater or surface water), they may not necessarily translate to additional water being available for future or downstream use. Consumptive use of water, water consumed and not returned to the local or sub-regional watershed, is not necessarily impacted by increased application efficiency, but is an important metric in conserving water for other uses. One recent irrigation technology …

Sensor-Based Irrigation Of Maize And Soybean In East-Central Nebraska Under A Sub-Humid Climate, Jasreman Singh, Derek M. Heeren, Yufeng Ge, Geng Bai, Christopher M.U. Neale, Mitchell S. Maguire, Sandeep Bhatti

Biological Systems Engineering: Papers and Publications

The ever increasing pressure on the water resources in Nebraska and other irrigated agricultural areas require innovations and solutions for the governance of water allocation. This study proposes the use of sensor-based method for irrigation which has the potential to improve irrigation water use efficiency (IWUE). Practical methods and algorithms for creating irrigation prescriptions have become vital for the adoption of precision irrigation. A decision support system (DSS) for uniform irrigation was evaluated during 2020 growing season in a sub-humid region. The DSS was managed using soil water and plant feedback. In field practice, a sensor node station comprising of …

Robotic Technologies For High-Throughput Plant Phenotyping: Contemporary Reviews And Future Perspectives, Abbas Atefi, Yufeng Ge, Santosh Pitla, James Schnable

Biological Systems Engineering: Papers and Publications

Phenotyping plants is an essential component of any effort to develop new crop varieties. As plant breeders seek to increase crop productivity and produce more food for the future, the amount of phenotype information they require will also increase. Traditional plant phenotyping relying on manual measurement is laborious, time-consuming, error-prone, and costly. Plant phenotyping robots have emerged as a high-throughput technology to measure morphological, chemical and physiological properties of large number of plants. Several robotic systems have been developed to fulfill different phenotyping missions. In particular, robotic phenotyping has the potential to enable efficient monitoring of changes in plant traits …

Comparison Of Promoter, Dna Vector, And Cationic Carrier For Efficient Transfection Of Hmscs From Multiple Donors And Tissue Sources, Tyler Kozisek, Andrew Hamann, Luke Samuelson, Miguel Fudolig, Angela K. Pannier

Biological Systems Engineering: Papers and Publications

Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be enhanced through genetic modification. However, gene delivery, particularly through nonviral routes, is inefficient. To address the shortcomings of nonviral gene delivery to hMSCs, our lab has previously demonstrated that pharmacological “priming” of hMSCs with clinically approved drugs can increase transfection in hMSCs by modulating transfection-induced cytotoxicity. However, even with priming, hMSC transfection remains inefficient for clinical applications. This work takes a complementary approach to addressing the challenges of transfecting hMSCs by systematically investigating key transfection parameters for their effect on transgene expression. Specifically, we investigated …

Modeling Vadose Zone Hydrology: Lecture Notes, Derek M. Heeren, Dean Eisenhauer

Open Educational Resources for Engineering

Modeling Vadose Zone Hydrology is a graduate-level course offered biennially in the Department of Biological Systems Engineering. Topics included hydraulic properties of porous media, application of Darcy's Law in variably saturated media, hydrologic and transport processes in the vadose zone, and solution of steady and unsteady flow problems using numerical techniques. A graphical approach for characterizing vertical one-dimensional problems with energy head profiles was emphasized. Common one-dimensional flow and transport problems were solved analytically. The course was taught using a combination of lecture notes and PowerPoint presentations. The lecture notes from 2021, captured using the Microsoft Whiteboard app with a …

Uavs For Vegetation Monitoring: Overview And Recent Scientific Contributions, Ana I. De Castro, Yeyin Shi, Joe Mari Maja, Jose M. Peña

Biological Systems Engineering: Papers and Publications

This paper reviewed a set of twenty-one original and innovative papers included in a special issue on UAVs for vegetation monitoring, which proposed new methods and techniques applied to diverse agricultural and forestry scenarios. Three general categories were considered: (1) sensors and vegetation indices used, (2) technological goals pursued, and (3) agroforestry applications. Some investigations focused on issues related to UAV flight operations, spatial resolution requirements, and computation and data analytics, while others studied the ability of UAVs for characterizing relevant vegetation features (mainly canopy cover and crop height) or for detecting different plant/crop stressors, such as nutrient content/deficiencies, water …

Early Detection Of Encroaching Woody Juniperus Virginiana And Its Classification In Multi-Species Forest Using Uas Imagery And Semantic Segmentation Algorithms, Lin Wang, Yuzhen Zhou, Qiao Hu, Zhenghong Tang, Yufeng Ge, Adam Smith, Tala Awada, Yeyin Shi

Biological Systems Engineering: Papers and Publications

Woody plant encroachment into grasslands ecosystems causes significantly ecological destruction and economic losses. Effective and efficient management largely benefits from accurate and timely detection of encroaching species at an early development stage. Recent advances in unmanned aircraft systems (UAS) enabled easier access to ultra-high spatial resolution images at a centimeter level, together with the latest machine learning based image segmentation algorithms, making it possible to detect small-sized individuals of target species at early development stage and identify them when mixed with other species. However, few studies have investigated the optimal practical spatial resolution of early encroaching species detection. Hence, we …