Bioresource and Agricultural Engineering Commons^™

Characterization Of Ground Nozzles For Pesticide Applications, Ryan Henry

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Pesticide applications are a common component of crop production systems in the United States (US). For row crop systems (e.g. corn, soybean, or wheat), pesticides are applied by ground, aerial, or chemigation methods. The exact method of pesticide delivery is not universally regulated/ prescribed in the US, and the equipment and application technique are largely defined by the individual applicator. A wide variety of choices and decisions must be made by applicators to result in a successful pesticide application. Examples of these choices include proper active ingredient(s), carrier volume and equipment (e.g. nozzle type, spacing, and operating pressure) selection while …

Deployment And Evaluation Of An Active Rfid Tracking System For Precision Animal Management, Brian Barnes

Department of Biological Systems Engineering: Dissertations and Theses

A better understanding of animal space utilization in current livestock facilities could lead to improved facility design and animal health. This study was conducted to determine whether an active RFID tag tracking system could accurately provide animal locomotion data on an individual animal basis. The system is composed of four sensors, located in the corners of a swine pen, and compact tags, which attach to the animals and transmit a signal. The sensors use the tag signals to determine 3-D positions in real-time. A data acquisition system was developed to capture raw data from the system software into a database …

Modeling Streambank Erosion On Composite Streambanks On A Watershed Scale, A. R. Mittelstet, D. E. Storm, G. A. Fox, P. M. Allen

Department of Biological Systems Engineering: Papers and Publications

Streambanks can be a significant source of sediment and phosphorus to aquatic ecosystems. Although the streambank-erosion routine in the Soil and Water Assessment Tool (SWAT) has improved in recent versions, the recently developed routine in SWAT 2012 has undergone limited testing, and the lack of site or watershed specific streambank data increases the uncertainty in the streambank-erosion predictions. There were two primary objectives of this research: (1) modify and test the 2012 SWAT streambank-erosion routine on composite streambanks, and (2) compare SWAT default and field-measured channel parameters and assess their influence on predicted streambank erosion. Three modifications were made to …

Quantifying An Aquifer Nitrate Budget And Future Nitrate Discharge Using Field Data From Streambeds And Well Nests, Troy E. Gilmore, David P. Genereux, Kathleen M. Farrell, Helena Mitasova

Department of Biological Systems Engineering: Papers and Publications

Novel groundwater sampling (age, flux, and nitrate) carried out beneath a streambed and in wells was used to estimate (1) the current rate of change of nitrate storage, dS_NO3 /dt, in a contaminated unconfined aquifer, and (2) future [NO₃^–]_FWM (the flow-weighted mean nitrate concentration in groundwater discharge) and f_NO3 (the nitrate flux from aquifer to stream). Estimates of dS_NO3 /dt suggested that at the time of sampling (2013) the nitrate storage in the aquifer was decreasing at an annual rate (mean = –9 mmol/m²yr) equal …

Effect Of Rainfall Timing And Tillage On The Transport Of Steroidhormones In Runoff From Manure Amended Row Crop Fields, Sagor Biswas, William L. Kranz, Charles A. Shapiro, Daniel D. Snow, Shannon L. Bartelt-Hunt, Mitiku Mamo, David D. Tarkalson, Tian C. Zhang, David P. Shelton, Simon J. Van Donk, Terry L. Mader

Department of Biological Systems Engineering: Papers and Publications

Runoff generated from livestock manure amended row crop fields is one of the major pathways of hormone transport to the aquatic environment. The study determined the effects of manure handling, tillage methods, and rainfall timing on the occurrence and transport of steroid hormones in runoff from the row crop field. Stockpiled and composted manure from hormone treated and untreated animals were applied to test plots and subjected to two rainfall simulation events 30 days apart. During the two rainfall simulation events, detection of any steroid hormone or metabolites was identified in 8–86% of runoff samples from any tillage and manure …

Flex-Ro: Design, Implementation, And Control Of Subassemblies For An Agricultural Robotic Platform, Jared Patrick Werner

Department of Biological Systems Engineering: Dissertations and Theses

Automation technology in agriculture is growing, making agricultural robotics viable. Innovative field usable multi-purpose robotic platforms are needed for the successful progression of agricultural robotics. Furthermore, the field of agricultural robotics would benefit from a robotic platform design allowing for variable height, thus accommodating navigation throughout various crop growth stages. A variable height machine, Flex-Ro was developed to accommodate this feature. Multiple sub-assemblies were designed and implemented for Flex-Ro. An electronic control unit (ECU) enabled engine was used to power Flex-Ro. An embedded application program was developed to control engine speed using proprietary Controller Area Network (CAN) messages in conjunction …

Lessons From The Far End: Caterpillar Frass-Induced Defenses In Maize, Rice, Cabbage, And Tomato, Swayamjit Ray, Saumik Basu, Loren J. Rivera-Vega, Flor E. Acevedo, Joe Louis, Gary W. Felton, Dawn S. Luthe

Department of Entomology: Faculty Publications

Plant defenses to insect herbivores have been studied in response to several insect behaviors on plants such as feeding, crawling, and oviposition. However, we have only scratched the surface about how insect feces induce plant defenses. In this study, we measured frass-induced plant defenses in maize, rice, cabbage, and tomato by chewing herbivores such as European corn borer (ECB), fall armyworm (FAW), cabbage looper (CL), and tomato fruit worm (TFW). We observed that caterpillar frass induced plant defenses are specific to each host-herbivore system, and they may induce herbivore or pathogen defense responses in the host plant depending on the …

Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, C. T. Agouridis, K. R. Douglas-Mankin, A. C. Linhoss, A. R. Mittelstet

Department of Biological Systems Engineering: Papers and Publications

Wetlands and coastal systems are unique, highly productive, and often threatened landscapes that provide a host of services to both humans and the environment. This article introduces a five-article Wetlands and Coastal Systems Special Collection that evolved from a featured session at the 2015 ASABE Annual International Meeting in New Orleans, Louisiana. The Collection provides perspectives on tools and techniques for enhancing the protection and restoration of wetlands and coastal systems with emphasis on vegetation, hydrology, water quality, and planning. Topics span the Florida Everglades (two articles) and Virginia floodplain (one article) wetland systems and include remote sensing (one article) …

Height And Pressure Test For Improving Spray Application, Anna Siebe, Joe D. Luck

UCARE Research Products

Pesticide application in agricultural fields affects a little over a million acres each year (USDA 2012). Current spray application equipment can automatically adjust nozzle flow rates in reaction to speed changes to maintain consistent application rates across the field. Uniform distribution of pesticides from the spray boom is critical to ensure proper crop care while minimizing negative environmental effects. Boom pressure and height are two primary factors that affect proper spray uniformity; however information on the combined effects of these factors are limited. The goal of this study was to provide end users with quantified data regarding the effects of …

Laboratory And Full Boom-Based Investigation Of Nozzle Setup And Restriction Effects On Flow, Pressure And Spray Pattern Distribution, Shane H. Forney

Department of Biological Systems Engineering: Dissertations and Theses

Pesticide application is an integral part of crop production and ground-based agricultural boom sprayers are used extensively to apply pesticides to the crop canopy or soil surface across millions of acres in the United States. Efficient application is necessary to minimize costs and limit adverse environmental impacts. Errors in flow rate and system pressure measurements may cause as-applied maps to incorrectly indicate application rates and could negatively affect downstream data processing or analysis. The goals of this study were to provide quantified measurements on the effects of nozzle setup errors on spray pattern uniformity and evaluate how laboratory patternator data …

Unmanned Aerial Vehicles For High-Throughput Phenotyping And Agronomic Research, Yeyin Shi, J. Alex Thomasson, Seth C. Murray, N. Ace Pugh, William L. Rooney, Sanaz Shafian, Nithya Rajan, Gregory Rouze, Cristine L. S. Morgan, Haly L. Neely, Aman Rana, Muthu V. Bagavathiannan, James Henrickson, Ezekiel Bowden, John Valasek, Jeff Olsenholler, Michael P. Bishop, Ryan Sheridan, Eric B. Putman, Sorin Popescu, Travis Burks, Dale Cope, Amir Ibrahim, Billy F. Mccutchen, David D. Baltensperger, Robert V. Avant, Jr., Misty Vidrine, Chenghai Yang

Department of Biological Systems Engineering: Papers and Publications

Advances in automation and data science have led agriculturists to seek real-time, high quality, high-volume crop data to accelerate crop improvement through breeding and to optimize agronomic practices. Breeders have recently gained massive data-collection capability in genome sequencing of plants. Faster phenotypic trait data collection and analysis relative to genetic data leads to faster and better selections in crop improvement. Furthermore, faster and higher-resolution crop data collection leads to greater capability for scientists and growers to improve precision-agriculture practices on increasingly larger farms; e.g., site-specific application of water and nutrients. Unmanned aerial vehicles (UAVs) have recently gained traction as agricultural …

High-Throughput Screening Of Clinically Approved Drugs That Prime Polyethylenimine Transfection Reveals Modulation Of Mitochondria Dysfunction Response Improves Gene Transfer Efficiencies, Albert Nguyen, Jared P. Beyersdorf, Jean-Jack Riethoven, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Nonviral gene delivery methods are advantageous over viral vectors in terms of safety, cost, and flexibility in design and application, but suffer from lower gene transfer efficiency. In addition to modifications to nucleic acid design and nonviral carriers, new tools are sought to enhance transfection. Priming is the pharmacological modulation of transfection efficiency and transgene expression, and has demonstrated transfection increase in several compounds, for example, chloroquine and glucocorticoids. To develop a library of transfection priming compounds, a highthroughput screen was performed of the NIH Clinical Collection (NCC) to identify clinical compounds that prime polyethylenimine (PEI) transfection. HEK293T cells were …

Carbon Nanotubes Affect The Toxicity Of Cuo Nanoparticles To Denitrification In Marine Sediments By Altering Cellular Internalization Of Nanoparticle, Xiong Zheng, Yinglong Su, Yinguang Chen, Rui Wan, Mu Li, Haining Huang, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Denitrification is an important pathway for nitrate transformation in marine sediments, and this process has been observed to be negatively affected by engineered nanomaterials. However, previous studies only focused on the potential effect of a certain type of nanomaterial on microbial denitrification. Here we show that the toxicity of CuO nanoparticles (NPs) to denitrification in marine sediments is highly affected by the presence of carbon nanotubes (CNTs). It was found that the removal efficiency of total NO_X⁻-N (NO₃⁻-N and NO₂⁻-N) in the presence of CuO NPs was only 62.3%, but it …

Water And Energy Use Of Antimicrobial Interventions In A Mid-Size Beef Packing Plant, Rami M. M. Ziara, Shaobin Li, Bruce I. Dvorak, Jeyamkondan Subbiah

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Data regarding the water and energy usage of current antimicrobial interventions in beef packing plants is scarce. The objective of this study was to collect representative water and energy usage data in a beef packing plant, with emphasis on antimicrobial interventions, to provide baseline data for comparison of new intervention technologies developed by researchers. Permanent and portable water flow meters were installed on the plant’s plumbing system to collect water flow data from March 2014 to March 2015. A local utility company was hired to meter electricity at the different subsystems using portable data loggers. The natural gas used in …

Using A Vnir Spectral Library To Model Soil Carbon And Total Nitrogen Content, Nuwan K. Wijewardane

Department of Biological Systems Engineering: Dissertations and Theses

n-situ soil sensor systems based on visible and near infrared spectroscopy is not yet been effectively used due to inadequate studies to utilize legacy spectral libraries under the field conditions. The performance of such systems is significantly affected by spectral discrepancies created by sample intactness and library differences. In this study, four objectives were devised to obtain directives to address these issues. The first objective was to calibrate and evaluate VNIR models statistically and computationally (i.e. computing resource requirement), using four modeling techniques namely: Partial least squares regression (PLS), Artificial neural networks (ANN), Random forests (RF) and Support vector regression …

Efficiency Of Chlorophyll In Gross Primary Productivity: A Proof Of Concept And Application In Crops, Anatoly A. Gitelson, Yi Peng, Andrés Viña, Timothy J. Arkebauer, James S. Schepers

Department of Biological Systems Engineering: Papers and Publications

One of the main factors affecting vegetation productivity is absorbed light, which is largely governed by chlorophyll. In this paper, we introduce the concept of chlorophyll efficiency, representing the amount of gross primary production per unit of canopy chlorophyll content (Chl) and incident PAR. We analyzed chlorophyll efficiency in two contrasting crops (soybean and maize). Given that they have different photosynthetic pathways (C3 vs. C4), leaf structures (dicot vs. monocot) and canopy architectures (a heliotrophic leaf angle distribution vs. a spherical leaf angle distribution), they cover a large spectrum of biophysical conditions. Our results show that chlorophyll efficiency in primary …

Toward Mining Massive And Multi-Dimensional Data For Extreme Hydrometeorological And Climate Event Analyses, Ling Qiu, Francisco Munoz-Arriola, Carlos M. Carrilo

UCARE Research Products

This poster presents the research on the main patterns of spatial distribution and temporal variability of precipitation and temperature in the Missouri River Basin (MRB). MRB has 117 million acres in cropland, produces of 46%, 22%, and 34% US's wheat, corn, and cattle, respectively. Also, MRB is known for intense weather and extreme climate variability. The approach is to identify different patterns of spatial distribution and temporal variability of precipitation and temperature through the use of a Principal Component Analysis. We have found that precipitation and temperature are the ideal meteorological variable to test spatiotemporal variability of extreme events using …

An Evaluation Of Agricultural Tractors Hydraulic Lift Performance, Grant Melotz

Department of Biological Systems Engineering: Dissertations and Theses

The current OECD Code 2 detailing the procedures for the hydraulic lift test of agricultural tractors, section 4.3, published lift values that were sometimes unattainable. The static weight of 2WD, two wheel drive, and MFWD, mechanical front wheel drive, tractors and the amount of lifting force have increased at a greater rate than the amount of static weight on the front axle. This increase in lifting force has led to a decrease in the percent of weight as the upward support force on the front axle of a tractor. Many of the 2WD and MFWD unballasted tractors tested at the …

Agroecosystem And Ecosystem Resiliency To Extreme Hydrometeorological And Climate Events, Katherine M. Werner

Department of Biological Systems Engineering: Dissertations and Theses

The purpose of this study was to understand how a large-scale representation of agroecosystems and ecosystems respond to extreme hydrometeorological and climate extreme events (EHCE) within the Platte River Basin. The ability for agroecosystems and ecosystems to adapt to a changing and variable climate is vital for global water, energy, and food security. Two model experiments were performed, the first with a time span from 2000 to 2013 in which a dynamic leaf area index (LAI) MODIS15A2 product was implemented. The second experiment ran from 1950 through 2013 used a climatological fixed seasonal cycle calculated as the average from the …

Tractor Measurement And Data Acquisition System For Hydraulic Power, Draft Force, And Power Take-Off Torque, James Roeber

Department of Biological Systems Engineering: Dissertations and Theses

Numerous advancements in machinery performance of agricultural tractors have been made in recent years. The Organisation for Economic Co-operation and Development (OECD) tests predetermined points (e.g., maximum power and torque) for drawbar, Power Take-Off (PTO), and hydraulic power as separate tests for tractor performance. Testing methods with the tractor operating at a steady state have been done for years, which were uncharacteristic of agricultural tractor operations in field conditions. As part of this thesis work, field usable data acquisition systems (DAQs) were developed to record implement energy consumption (e.g., drawbar loading, PTO torque, and hydraulic power). The system used LabVIEW …

Science-Informed Arguments In Undergraduates' Opinions About Biofuels, Olivia Straka, Jenny Dauer

UCARE Research Products

Socioscientific issues (SSIs) are complex, multifaceted issues with components of science as well as components of values requiring evaluation of social, political, and ethical concerns (Nichols & Zeidler, 2009). • However, many students do not possess a fundamental understanding of the underlying scientific foundations of SSIs (NRC, 1998), or may evaluate issues based on personal relevance as opposed to contemplation of evidence presented (Sadler et al., 2004). • This study examined how undergraduate students chose to substantiate their opinions on the SSI of using biofuels as a fuel source before and after taking a semester long course, AGRI/NRES 103. • …

Engineering Outreach Education Through Social Media, Katie E. Meiergerd, Dagen Valentine, Jennifer R. Keshwani, Bradley Barker

UCARE Research Products

Familiarizing teachers and educators with Engineering and other related STEM fields is crucial in educating students, so ensuring teachers with access to these resources is important. The objective of this work is to identify ways to use social media to provide support to K – 12 educators interested in implementing engineering education with their students.

Exploring Large-Scale Parameterization Of Irrigation In The Northern High Plains, Mitch Maguire, Daniel Rico, Katherine Smith, Francisco Munoz-Arriola

UCARE Research Products

Research Question – Objective - Hypothesis

How does irrigation respond to the demands of Crop Irrigation Requirements under extreme hydrometeorological events?

Implement a parameterization of irrigation into the land surface hydrology model VIC.

Changes in evapotranspiration (ET) will be more conspicuous during dry years, highlighting the limiting role of soil moisture in the model.

Micro- And Nanoparticulates For Dna Vaccine Delivery, Eric Farris, Deborah M. Brown, Amanda Ramer-Tait, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

DNA vaccination has emerged as a promising alternative to traditional protein-based vaccines for the induction of protective immune responses. DNA vaccines offer several advantages over traditional vaccines, including increased stability, rapid and inexpensive production, and flexibility to produce vaccines for a wide variety of infectious diseases. However, the immunogenicity of DNA vaccines delivered as naked plasmid DNA is often weak due to degradation of the DNA by nucleases and inefficient delivery to immune cells. Therefore, biomaterial-based delivery systems based on micro- and nanoparticles that encapsulate plasmid DNA represent the most promising strategy for DNA vaccine delivery. Microparticulate delivery systems allow …

Ecosystem Evapotranspiration: Challenges In Measurements, Estimates, And Modeling, D.M. Amatya, Suat Irmak, P. Gowda, G. Sun, J.E. Nettles, K.R. Douglas-Mankin

Department of Biological Systems Engineering: Papers and Publications

Evapotranspiration (ET) processes at the leaf to landscape scales in multiple land uses have important controls and feed backs for local, regional, and global climate and water resource systems. Innovative methods, tools, and technologies for improved understanding and quantification of ET and crop water use are critical for adapting more effective management strategies to cope with increasing demand for freshwater resources under global climate change. This article introduces an ASABE Special Collection of 12 articles on ET monitoring and modeling research for multiple land uses and scales. The collection focuses on recent advances in four critical topical areas: (1) reference …

Glucocorticoid Cell Priming Enhances Transfection Outcomes In Adult Human Mesenchymal Stem Cells, Abby M. Kelly, Sarah A. Plautz, Janos Zempleni, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Human mesenchymal stem cells (hMSCs) are one of the most widely researched stem cell types with broad applications from basic research to therapeutics, the majority of which require introduction of exogenous DNA. However, safety and scalability issues hinder viral delivery, while poor efficiency hinders nonviral gene delivery, particularly to hMSCs. Here, we present the use of a pharmacologic agent (glucocorticoid) to overcome barriers to hMSC DNA transfer to enhance transfection using three common nonviral vectors. Glucocorticoid priming significantly enhances transfection in hMSCs, demonstrated by a 3-fold increase in efficiency, 4–15-fold increase in transgene expression, and prolonged transgene expression when compared …

Informative Spectral Bands For Remote Green Lai Estimation In C3 And C4 Crops, Oz Kira, Anthony L. Nguy-Robertson, Timothy J. Arkebauer, Raphael Linker, Anatoly A. Gitelson

School of Natural Resources: Faculty Publications

Green leaf area index (LAI) provides insight into the productivity, physiological and phenological status of vegetation. Measurement of spectral reflectance offers a fast and nondestructive estimation of green LAI. A number of methods have been used for the estimation of green LAI; however, the specific spectral bands employed varied widely among the methods and data used. Our objectives were (i) to find informative spectral bands retained in three types of methods, neural network (NN), partial least squares (PLS) regression and vegetation indices (VI), for estimating green LAI in maize (a C4 species) and soybean (a C3 species); (ii) to assess …

Early Life History Of Three Pelagic-Spawning Minnows Macrhybopsis Spp. In The Lower Missouri River, T. A. Starks, M. L. Miller, J. M. Long

US Army Corps of Engineers

Life-history characteristics of age-0 sturgeon chub Macrhybopsis gelida, shoal chub Macrhybopsis hyostoma and sicklefin chub Macrhybopsis meeki were compared using several methods. All Macrhybopsis species consumed mostly midge pupae, but M. meeki had the most general diet (Levins’ index, B=0⋅22) compared with M. hyostoma (B=0⋅02) and M. gelida (B=0⋅09). Morisita’s diet overlap index among species pairs ranged from 0⋅62 to 0⋅97 and was highest between M. hyostoma and M. gelida. Daily ages estimated from lapilli otoliths for each species ranged from 15 to 43 days for M. gelida, 19 to 44 for …

2016 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman

Nebraska Department of Environmental Quality: Reports

The Nebraska Department of Environmental Quality (NDEQ) is charged with monitoring, assessing, and to the extent possible, managing the state’s water resources. The purpose of this work is to protect and maintain high quality water and encourage or execute activities to improve poor water quality. Monitoring is done on nearly 17,000 miles of flowing rivers and streams, more than 134,000 acres of surface water in lakes and reservoirs, as well as the vast storage of groundwater in Nebraska’s aquifers.

2016 Nebraska Groundwater Quality Monitoring Report

Nebraska Department of Environmental Quality: Reports

The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …